Hormonal regulation of endometrial olfactomedin expression and its suppressive effect on spheroid attachment onto endometrial epithelial cells

Abstract

Decreased expression of Olfm during the receptive phase of the endometrium may allow successful trophoblast attachment for implantation.

Ovarian stimulation modulates steroid receptor expression and spheroid attachment in peri-implantation endometria: studies on natural and stimulated cycles

“…To demonstrate how the E 2 and P levels affect spheroid attachment, an initial step of implantation, we established a spheroidendometrial cell coculture model (13,14). Our study showed that excessively high E 2 and P levels suppressed trophoblastic spheroid attachment onto endometrial epithelial cell lines, resembling the clinical situation as demonstrated in earlier studies (8,29,30).…”

“…Adhesion of Jeg-3 spheroids to endometrial Ishikawa cells was quantified by using an adhesion assay as described elsewhere (11,13). In the coculture study, the medium of Jeg-3 and Ishikawa cells were changed to phenol red-free DMEM/F12 supplemented with 10% charcoal-stripped fetal bovine serum.…”

Human chorionic gonadotropin stimulates spheroid attachment on fallopian tube epithelial cells through the mitogen-activated protein kinase pathway and down-regulation of olfactomedin-1

“…A high P level is associated with a lower Olfm1 expression level during the implantation window in human endometrium, and suppression of Olfm1 expression enhances spheroid attachment in OE-E6/E7 cells (36). Therefore, steroid hormones likely modulate the effect of embryo-derived hCG in activating the hCG/LH receptor, through the ERK/Wntsignaling pathway, to suppress Olfm1 expression in human fallopian tubes.…”

“…In zebrafish, Olfm1 regulated the Wnt-signaling pathway and modulated retinal axon elongation (35). Previously, we used a trophoblastic spheroid (JAr)-endometrial epithelial cell (Ishikawa) co-culture model to demonstrate the suppressive effect of Olfm1 on JAr spheroid attachment on Ishikawa cells (36). However, the way in which Olfm1 is regulated at the feto-maternal interface remains largely unknown.…”

Two-dimensional liquid chromatography with tandem mass spectrometry–based proteomic characterization of endometrial luminal epithelial surface proteins responsible for embryo implantation

“…Nonspecific binding was blocked with 5% bovine serum albumin diluted in phosphate-buffered saline. The sections were then successively incubated with antibodies against APN (Abcam), integrin-b1 (Millipore), Mucin4 (Abcam), or vitroectin (Abcam), and respective horseradish peroxidase conjugated secondary antibodies (DAKO) using the procedures described previously elsewhere (17). Biotinylated cells were detected by the Vectastain ABC reagent (Vector Laboratories) and 3,3 0 -diaminobenzidine solution (DAKO).…”