Hormonal monitoring of ovarian activity using the Ovarian Monitor, Part I. Validation of home and laboratory results obtained during ovulatory cycles by comparison with radioimmunoassay

Blackwell, Leonard F.; Brown, J. B.; Vigil, Pilar; Gross, Barbara A.; Sufi, S.; d’Arcangues, Catherine

doi:10.1016/s0039-128x(03)00049-7

Cited by 38 publications

(34 citation statements)

References 14 publications

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“…LH and progesterone trigger follicular-wall degradation by proteases, prostaglandins, and other compounds (Stouffer 2003), resulting in release of the oocyte which normally survives 12 to 24 hours (Ferin, Van Vught, and Wardlaw 1984). After ovulation, LH contributes to the development of the corpus luteum (Misao et al 1998), which continues to produce progesterone and estrogen during the luteal phase (Blackwell et al 2003). Progesterone modifies the endometrial lining from proliferative to secretory type and changes the cervical mucus from estrogenic to progestational.…”

Section: Endocrine Regulation Of the Ovulatory Cyclementioning

confidence: 99%

Ovulation, A Sign of Health

Vigil

Lyon²,

Flores³

et al. 2017

Linacre Q

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The concept of the ovarian continuum can be understood as a process that occurs during a woman's lifetime and begins during intrauterine life with fertilization. Women start their reproductive years with approximately five hundred thousand follicles containing oocytes, of which only around five hundred will be released during ovulation. Ovulation has been recognized as an event linked with reproduction; however, recent evidence supports the role of ovulation as a sign of health. The use of biomarkers that help women recognize ovulation enables them to identify their health status. This knowledge helps medical healthcare providers in the prevention, diagnosis, and treatment of different pathologies related with endocrine disorders, gynecological abnormalities, autoimmune, genetic, and neoplastic diseases, as well as pregnancy-related issues. The knowledge of the ovarian continuum and the use of biomarkers to recognize ovulation should be considered a powerful tool for women and medical professionals. Summary:The ovarian continuum is a process that occurs during a woman's lifetime. It begins during intrauterine life with fertilization and ends with menopause. This process can be greatly affected by different conditions such as changes in hormonal levels and illnesses. Therefore, understanding and promoting the knowledge and use of biomarkers of ovulation in women is a key aspect to consider when evaluating their health status. The knowledge and education about the ovarian continuum should be taken into account as a powerful tool for women and medical professionals.

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Section: Endocrine Regulation Of the Ovulatory Cyclementioning

confidence: 99%

Ovulation, A Sign of Health

Vigil

Lyon²,

Flores³

et al. 2017

Linacre Q

Self Cite

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“…29 To date a total of 20 distinct mucin genes have been identified. 30 Mucins are categorized into 3 groups on the basis of their structural properties such as transmembrane mucin (MUCs 1, 2, 3, 4, 12, 13, 15, 16, 17), gel-forming mucin (MUCs 2, 5AC, 5B, 6) and soluble mucin ( MUCs 7,9,11,14) and others that have not yet been categorized (MUCs 8, 10, 18, 19, and 20). 30 The four large gel-forming mucin genes are located on chromosome 11.p15.5.…”

Section: Cervical Mucus As a Determinant For Fertilitymentioning

confidence: 99%

Usefulness of Monitoring Fertility from Menarche

Vigil

Cerić

Cortés

et al. 2006

Journal of Pediatric and Adolescent Gynecology

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“…In particular, estrone glucuronide (E1G) and pregnanediol glucuronide (PdG) conjugates of lysozyme have been used in the Ovarian Monitor [1] to monitor the main urinary estradiol and progesterone metabolites and, thus, define the fertile period of the human menstrual cycle. [1 -3,7] The most readily acylated lysine residues in HEWL (K33, K97 and K116) are all situated close to its welldefined active site cleft [4,5] and HEWL is relatively small compared to its bacterial cell substrate Micrococcus lysodeikticus (M. lysodeikticus).…”

Section: Introductionmentioning

confidence: 99%

“…The inhibition of the HEWL-E1G and HEWL-PdG conjugate lytic activity is prevented in a dose-dependent manner by pre-incubation of the antibody with the appropriate free hapten from either standards or from urine samples. [1] Thus, there is a direct relationship between the lytic activity of the assay system and the steroid glucuronide concentration in a sample. The Monitor utilises an endpoint assay for measurement of E1G which is calculated from the difference in transmission values (DT) taken 20 minutes apart [1 -3,5,7] as the suspension of M. lysodeikticus clears during the lytic reaction.…”

Section: Introductionmentioning

confidence: 99%

“…The Monitor utilises an endpoint assay for measurement of E1G which is calculated from the difference in transmission values (DT) taken 20 minutes apart [1 -3,5,7] as the suspension of M. lysodeikticus clears during the lytic reaction. From a simple measurement of DT over 20 minutes, the accurate E1G excretion rate [1] can be obtained by reference to the appropriate standard curve.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Clearing of Suspensions ofMicrococcus lysodeikticusCatalysed by Lysozymes from Hen, Goose, and Turkey Egg Whites, Human Milk, and Phage T4. Assessment of Potential as Signal Generators for Homogeneous Enzyme Immunoassays for Urinary Steroids

Cooke

Blackwell

2007

Journal of Immunoassay and Immunochemistry

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Lysozymes (3.2.1.17) from goose (Anser anser) egg white, turkey (Melagris gallopavo) egg white, phage T4 and human milk were compared with hen egg white lysozyme in their ability to clear a suspension of Micrococcus lysodeikticus. All of the lysozymes, except hen egg white lysozyme, catalysed the clearing of the Micrococcus lysodeikticus suspension in a biphasic fashion. Compared to hen egg white lysozyme, the total absorbance or transmission change over 5 and 20 minutes was less in all cases, except for human lysozyme. Human lysozyme was, therefore, a potential alternative, more rapid signal generator for the measurement of urinary estrone glucuronide excretion rates because of its structural similarity to hen egg white lysozyme. The apparent K(M) values for hen egg white lysozyme increased with the enzyme concentration.

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Hormonal monitoring of ovarian activity using the Ovarian Monitor, Part I. Validation of home and laboratory results obtained during ovulatory cycles by comparison with radioimmunoassay

Cited by 38 publications

References 14 publications

Ovulation, A Sign of Health

Ovulation, A Sign of Health

Usefulness of Monitoring Fertility from Menarche

Clearing of Suspensions ofMicrococcus lysodeikticusCatalysed by Lysozymes from Hen, Goose, and Turkey Egg Whites, Human Milk, and Phage T4. Assessment of Potential as Signal Generators for Homogeneous Enzyme Immunoassays for Urinary Steroids

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