Hormonal induction of supermale golden rosy barb and isolation of Y-chromosome specific markers

Embryonic development of the rosy barbPuntius conchoniusHamilton 1822 (Cyprinidae)

“…It is native to rivers and fast flowing streams of Afghanistan, Pakistan, India, Nepal and Bangladesh and is now very popular with aquarists throughout the world. The rosy barb has a short generation time and produces great numbers of large, transparent eggs that are fertilized externally (RIJKERS 1980, AMANZE & IYENGAR 1990, KIRANKUMAR et al 2003. It is therefore becoming a potential experimental fish model for biological and biotechnological research.…”

“…It is therefore becoming a potential experimental fish model for biological and biotechnological research. In fact it has been widely used in ecotoxicological studies in recent years (GILL et al 1990(GILL et al , 1991a(GILL et al , 1991bADAM et al 1995;VARADI et al 1995;KIRANKUMAR et al 2003).…”

Expression Analysis of <b><i>sox3</i></b> during Testicular Development, Recrudescence, and after hCG Induction in Catfish, <b><i>Clarias batrachus</i></b>

“…Recent studies showed that loss of Sox3 causes germ cell depletion in developing mouse testis [Laronda and Jameson, 2011] and can activate the male pathway in the same way as Sry in the absence of a Y chromosome and facilitate testicular development in mice and human [Sutton et al, 2011]. Such a possibility may exist in lower vertebrates, wherein no SRY-like molecules were found, except for the existence of Y-chromosome specific molecular markers in a few teleosts [Devlin and Nagahama, 2002;Kirankumar et al, 2003]. Hence, Sox3 alone or in combination with other transcription factors can drive the male pathway either directly or through the regulation of steroidogenic enzymes vis-à-vis sex steroid levels.…”

Molecular characterization and chromosomal localization of spermatogenesis related sequences in Torpedo torpedo (Chondrichthyes, Torpediniformes)

“…In fact, different types of markers have only been characterized in few species; i.e., the specific probe of the chromosome Y in salmon Oncorhynchus tshawytscha (Devlin et al, 1991), the specific repetitive sequence of the sex chromosome in the Poeciliids (Nanda et al, 1993), specific quantitative sex markers in O. tshawytscha (Clifton and Rodriguez, 1997) and the pseudogene for the growth hormone (GH-φ) in salmon Oreochromis masou masou (Zhang et al, 2001). Other than in Salmonids and Poecilids, specific molecular markers have been identified, for the first time, on the chromosome Y of the cyprinid Puntius conchonius (Kirankumar et al, 2003). The authors assumed that the fragment of 200 bp that they found in P. conchonius could be associated with autosomal elements, which are known to have modified the normal process of sex determination.…”

“…The PCR reaction was carried out using the primers SRY F1 (5′ GAGAGCATGGAGGGCCAT 3′) and SRY R1 (5′ CCACTCCTCTGTGACACT 3′-) (Kirankumar et al, 2003) on genomic DNA (25 ng each) of the male and of the female of the species T. torpedo to identify the sex-specific markers. The PCR protocol followed was: initial denaturation at 94°C for 3 min, denaturation at 94°C for 30 s, annealing at 56°C for 30 s, extension at 72°C for 1 min, final extension at 72°C/5 min (40 reaction cycles).…”