Hormonal Induction of Ovulation In Vitro in Sturgeon Fishes

Stimulation of in vitro oocyte ovulation by progesterone and homologous pituitary gonadotropic hormone in sturgeons

“…It is quite probable that the best result can be obtained upon the use of Leibovitz medium rather than RMS at a certain state of follicles and the reverse at another state. At the present time, during work with unique females, it seems expedient to obtain in vitro mature, ovulated, and capable of development eggs to use in parallel also the described previously (Goncharov et al, 2009) and described in this paper optimal conditions for cultiva tion of follicles and their hormonal treatment.…”

“…In the previous study (Goncharov et al, 2009) ded icated to the elaboration of the method of hormonal induction of ovulation in vitro we found that it is pos sible to obtain a high percent of ovulated oocytes of stellate sturgeon with a decrease in osmolality of the cultivation medium of follicles via dilution of RMS; however, under these conditions, the process of their maturation is disturbed. Such oocytes are incapable of fertilization and/or development.…”

“…After our study on stellate sturgeon (Goncharov et al, 2009), we got an impression that such simple salt medium as RMS is not capable of promoting the for mation or providing the maintenance of the capacity of eggs for development despite the fact that it is pos sible to induce in it both maturation and ovulation of oocytes. Recall that in the cited paper a considerably better result was obtained upon the use of the diluted Leibovitz medium.…”

“…The study of the effect of the composi tion of the culture medium of ovarian follicles on the ovulation of oocytes of stellate sturgeon demonstrated that one of the factors affecting the result of hormonal stimulation in vitro is the medium osmolality. The per centage of oocytes of stellate sturgeon ovulating in vitro under the effect of an extract of sturgeon pituitar ies or progesterone increased after dilution of the cul ture medium (Goncharov et al, 2009). As initial media in this study, we used Ringer solution for cold blooded animals modified for sturgeons (RMS) (Goncharov, 1978) and Leibovitz medium (L 15) with the addition of sodium bicarbonate.…”

Obtaining gynogenetic progeny of Siberian sturgeon (Acipenser baerii) using eggs matured and ovulated in vitro

“…The biopsy of ovarian follicles is sufficiently harmless to the female to be repeated until a desirable result is obtained. The use of this method on the stellate sturgeon ( Acipenser stellatus ) has shown that up to 100% of oocytes incubated in the presence of hormone can mature, up to 90% can ovulate, and about 60% of inseminated eggs are able to develop up to the larval feeding stage (the end point of observation) (Goncharov et al., 2009).…”