Hormonal induction of lactation in prepuberal and multiparous crossbred goats kept under extensive conditions

Mellado, M.; Bernal, Andrés López; Mendoza, Roberto; Carrillo, Evaristo

doi:10.1016/0921-4488(95)00746-6

Cited by 5 publications

(4 citation statements)

References 17 publications

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“…The successful lactation rate of 100% verified in this study was similar to the results obtained in other induced trials (9) . Furthermore, the lactation initiated in a similar period that has alrady been cited for goats (3) .…”

Section: Resultssupporting

confidence: 79%

Mammary Biopsy in Induced Lactating Goats: Assessment of Post-Biopsy Milk Properties and Ultrasonographic Appearance

et al. 2017

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The aim of this study was to evaluate the effects of mammary biopsy procedures on gland function of goats submitted to hormonal lactation. Ten female Caninde goats, at the age of around 3 years, were used, and lactation was induced by using estrogen, progesterone, and prednisolone. Ultrasonographic examinations of mammary gland, milking, and analysis of physicochemical properties of milk were performed immediately before (0 h) and 24, 48, and 72 h after each biopsy procedure. Surgical mammary gland biopsies were obtained at days 5 and 26 of the lactation. Even after drying initiation, all animals produced milk for five months. No abnormalities were observed on the ultrasonographic appearance of biopsied glands. All 20 biopsies were technically successful and any inflammation or infection was associated with biopsy procedures. Regarding the physicochemical properties and volumes of milk produced, no significant differences (P > 0.05) were observed between right and left glands nor in the comparison of the results from time 0 with 24, 48, and 72 h for biopsied teats. The mammary biopsy did not induce neither severe changes in production and physicochemical properties of the milk nor in ultrasonographic appearance of mammary gland of goats in hormonal lactation.Keywords: biopsy; goat; udder; ultrasonography. ResumoO objetivo deste estudo foi avaliar os efeitos dos procedimentos de biópsia mamária sobre a função da glândula de caprinos submetidos à lactação induzida. Dez cabras Canindé com idade de cerca de 3 anos foram utilizadas e a lactação foi induzida pelo uso de estrógeno, progesterona e prednisolona. Exames ultrassonográficos da glândula mamária, ordenha e análise das propriedades físico-químicas do leite foram realizados imediatamente antes (0 h) e 24, 48 e 72 h após cada procedimento de biópsia. As biópsias cirúrgicas de glândula mamária foram obtidas aos dias 5 e 26 da lactação. Mesmo após o início do processo de secagem, todos os animais continuaram produzindo leite por cinco meses. Nenhuma anormalidade foi observada na aparência ultrassonográfica das glândulas biopsiadas. Todas as 20 biópsias foram realizadas com sucesso e nenhuma inflamação ou infecção foi observada. Com relação às propriedades físico-químicas e volumes de leite produzido, nenhuma diferença significativa Cienc. anim. bras., Goiânia, v.18, 1-7, e-39077, 2017 2 SOUSA, F.C. et al.(P > 0,05) foi observada entre a glândula direita e esquerda, nem comparando resultados do momento 0 com 24, 48 e 72 h para as glândulas biopsiadas. A biópsia mamária não induz severas mudanças na produção e nas propriedades físico-químicas do leite nem na aparência ultrassonográfica da glândula mamária de caprinos em lactação.

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Section: Resultssupporting

confidence: 79%

Mammary Biopsy in Induced Lactating Goats: Assessment of Post-Biopsy Milk Properties and Ultrasonographic Appearance

et al. 2017

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“…20 This unwanted side effect should be thought about if inclusion of COU or DMSO is to be considered in reproductive control programs. The induced abnormal mammary gland development and galactorrhea were not considered as signs of disease in this study, since use of estrogenic compounds combined with P4 is frequent to induce lactations in infertile ruminants, [36][37][38] in non-human primates 39 and in human surrogate pregnancies. 40 Moreover, care was taken to instruct the owners on how to check for behavioral changes typical of pseudopregnancy, such as circling, digging or nesting, but none were seen in any of the bitches presenting with mammary alterations in size and/or function (Figure 4).…”

Section: Health and Side Effectsmentioning

confidence: 99%

Effect of a single application of coumestrol and/or dimethyl sulfoxide, on sex hormone levels and vaginal cytology of anestrus bitches

et al. 2019

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Canine overpopulation continues to be a problem with serious public health implications, despite a diversity of programs and strategies that have been implemented for its control. Coumestrol (COU) is an organic compound with estrogenic activity, thus having the potential to alter reproduction in mammals. COU is commonly dissolved in dimethyl sulfoxide (DMSO) before administration; however, evidence indicates that DMSO is not inert. The aim of this study was to assess the effects of either a single oral administration of COU diluted in DMSO or of DMSO-alone, on serum progesterone (P4) and estradiol (E2), on vaginal cell pattern, and on anestrus and diestrus lengths in bitches. Fifteen anestrus female dogs received either a single commercial dog food biscuit (Control, n=5), a biscuit with 600 μg of COU/kg diluted in 20 μL of DMSO (COU, n=5), or a biscuit with 20 μL of DMSO (DMSO, n=5). Circulating P4, E2, and changes in vaginal cytology, were assessed within the first month after treatment administration. Hormone levels were also measured from months 2-6 post-treatment. Mean differences were analyzed by the GLM procedure for repeated measures. COU enhanced serum E2 levels, and DMSO increased serum P4, number of vaginal anucleated superficial cells, and diestrus length. All dogs were deemed healthy based on all periodical clinical exams, but abnormal mammary gland growth and/or galactorrhea were observed in two COU and one DMSO-treated bitches. The findings of the present study expose the need to reevaluate previous reports of use of COU in bitches, and perhaps in other mammals.Figure 1. Progesterone (A) and estradiol (B) serum levels in anoestrous bitches receiving a commercial dog food biscuit alone (Control; n=5), a biscuit with coumestrol diluted in dimethyl sulfoxide (COU; n=5), or a biscuit with only dimethyl sulfoxide (DMSO; n=5). a,b Different letters within sampling day indicate difference between treatments (P<0.05).*specifies statistical increase on post-treatment days 21 (P<0.0048) and 28 (0.0008). Data are presented as LSM ± standard error.

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“…Milk compositions during natural and hormonally induced lactations have also been reported (Ryot, 1990). Previous reports have studied the effects of hormonal induction on prepuberal goats kept under field conditions (Mellado, 1996), while others have looked at histomorphology of the gland after prepubertal induction of lactation (Ryot, 1989). Additionally, hormonal induction of lactation in adult transgenic animals has been reported (Ebert, 1994).…”

Section: Introductionmentioning

confidence: 98%

Hormonal induced lactation in transgenic goats

Cammuso¹,

Porter²,

Nims³

et al. 2000

Animal Biotechnology

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The aim of this study was to hormonally induce lactation in prepubertal, nulliparous, and male goats both transgenic and non-transgenic. Analysis of milk quality, recombinant protein expression levels, total amount of recombinant protein produced, and the affect on long-term reproductive capability was assessed. Fifty-one goats (Saanen, Alpine, and Toggenburg), male and non-pregnant females, 2-31 months of age, either non-transgenic or transgenic were evaluated with a total of 10 transgenes (constructs) represented. Animals were given estradiol (0.25 mg/kg, i.m.) and progesterone (0.75 mg/kg, i.m.) on days 1, 3, 5, 7, 9, 11 and 13, while prednisilone (0.4 mg/kg, i.m.) was administered on days 14-16 with mammary massage occurring daily from day 5 onward. Forty of 51 animals, (36 of 38 females and 4 of 13 males) produced milk with total volumes in the 30-day experiment, ranging from 20 microl to 530 mls per day, or approximately 500 microl to 6.8 liters total. Milk composition was analyzed for various parameters (total protein, fat content, total solids and somatic cell count) with no significant differences found between induced and natural milk. Expression levels of recombinant proteins from transgenic animals that were analyzed during the induced lactation, and subsequently during normal lactations, were found to have no significant differences. Total amount of recombinant protein produced was evaluated at different expression levels with no statistical significance seen. While over 90% of the females placed in the regimen became pregnant, there was a correlation between increased age at time of induction and an increase in number of breedings, or reproductive cycles needed to establish a pregnancy after induction. For males, 100% placed in the regimen settled females after hormonal induction of lactation. Semen quality was evaluated prior to, during, and after hormonal treatments. Semen volume and sperm number did not differ; however, for a small percentage of males, there was a decrease in sperm and post thaw motility after hormonal treatments. These levels returned to normal within 4-5 weeks. Subsequent natural lactations showed total milk volumes within breed standards. These findings indicate that hormonal induction of lactation in the caprine species is a viable alternative to pregnancy for initiating lactation and milk production, does not adversely impact reproductive performance long-term, and can benefit the early assessment of recombinant proteins produced in a transgenic founder program.

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Hormonal induction of lactation in prepuberal and multiparous crossbred goats kept under extensive conditions

Cited by 5 publications

References 17 publications

Mammary Biopsy in Induced Lactating Goats: Assessment of Post-Biopsy Milk Properties and Ultrasonographic Appearance

Mammary Biopsy in Induced Lactating Goats: Assessment of Post-Biopsy Milk Properties and Ultrasonographic Appearance

Effect of a single application of coumestrol and/or dimethyl sulfoxide, on sex hormone levels and vaginal cytology of anestrus bitches

Hormonal induced lactation in transgenic goats

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