Hormonal Effects on the Quantitative Transplantation of Monodispersed Rat Thyroid Cells*

Mulcahy, Ríona; Rose, David P.; Mitchen, Joan M.; Clifton, Kelly H.

doi:10.1210/endo-106-6-1769

Cited by 14 publications

(3 citation statements)

References 7 publications

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“…Although, as in the liver (291), most of the thyrocytes in the thyroid appear to be able to respond by a few divisions to a proliferation stimulus in vivo and in vitro, the existence of a population of stem cells able to replenish the pool of fully differentiated and proliferationlimited thyrocytes has been postulated. This hypothesis rests on indirect but suggestive evidence: I) the growth of thyroid transplants in recipient animals requires the injection of a minimal number of cells (219), which might measure the relative frequency of stem cells; and 2) in cloning assays in radiobiological experiments, foci formation occurs but with very low efficiency. From both types of experiments the frequency of stem cells would be estimated at most to be l/1,000.…”

Section: Stem Cellsmentioning

confidence: 99%

Physiological and pathological regulation of thyroid cell proliferation and differentiation by thyrotropin and other factors

Dumont

Lamy

Roger

et al. 1992

Physiological Reviews

526

337

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I. CELL PROLIFERATION IN NORMAL THYROID TISSUE cles and of their supporting mesenchymal tissue and IN VIVO cells, the endothelial cells of the capillaries (20%) and the fibroblasts (10%) (78). Scarce calcitonin-secreting A. Physiological Situation parafollicular cells are located at the periphery of the follicles. After its differentiation in the fetus, the tissue The thyroid tissue is mainly composed of thyroid grows roughly in parallel with body weight and remains follicular cells, the thyrocytes (70954, arranged in folli-at the same size throughout adult life.

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Section: Stem Cellsmentioning

confidence: 99%

Physiological and pathological regulation of thyroid cell proliferation and differentiation by thyrotropin and other factors

Dumont

Lamy

Roger

et al. 1992

Physiological Reviews

526

337

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“…Surgical procedures and blood collections were performed under light ether anesthesia and the rats were killed by ether overdose. To maximize the efficiency of FU formation following transplantation, recipient animals were surgically thyroidectomized (tx) before grafting (Mulcahy et al 1980). Cell Monodispersion and Transplantation for Assays of Clonogen Concentration: The technique for enzymatic monodispersion and transplantation of thyroid cells has been described (Clifton and Gould 1985).…”

Section: Methodsmentioning

confidence: 99%

On the Cells of Origin of Radiogenic Thyroid Cancer: New Studies Based on an Old Idea

Clifton¹,

Domann²,

Groch³

1991

JRR

Self Cite

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We have presented evidence that the functional thyroid follicles (follicular units, FU) which are formed in grafts of monodispersed rat thyroid cells, and hence the thyroid tumors which later develop in such grafts, are clonal in origin. Transplantation assays indicate that the clonogens comprise -1% of the cells in monodis persed suspensions of normal thyroid tissue. Carcinogenesis studies show that neoplastic initiation of thyroid clonogens by radiation is a common event. Promotion-progression to cancer from radiation initiated clonogens has, however, been shown to be inversely related to the total grafted thyroid cell number; i.e. more tumors develop per irradiated clonogen in grafts of small cell numbers than of large cell numbers. Recent studies have been designed to investigate: a) whether the cell number-dependent inhibition of promotion-progres sion is mediated by remote hormonal feed-back, local cell-cell interactions, or both; b) the cell population kinetics of the clonogen subpopulation during goitrogenesis and goiter involution; and c) the effect of prolonged exposure to high levels of TSH (thyrotropin) on the capacity of the clonogens to give rise to functional FU. The results indicate that local cell-cell interactions play an important role in the cell number dependent suppression of neoplastic promotion-progression. They also show that if sufficient thyroid cells are grafted, the thyroid-pituitary axis can be reestablished in thyroidectomized rats fed normal diets. In such animals given iodine deficient diets, the FU that develop in the thyroid grafts shift their secretory pattern to increase the ratio of T3 (triiodothyronine) to T4 (thyroxine), and thus conserve the available iodine. Finally, the clonogenic subpopulation is conserved during both goitrogenesis and goiter involution. When they are transplanted to thyroidectomized recipients, clonogens from two types of goiters form FU that are mor phologically indistinguishable from those that develop in grafts of normal thyroid clonogens. Furthermore, the secretion of T3 and T4 by such grafts is dependent on the grafted clonogen number, and hence FU formation, and not on the total number of thyroid cells transplanted. We conclude that the thyroid clono gens, the presumptive cancer progenitor cells, have many of the characteristics of stem cells.

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“…The thyroid gland is, therefore, capable of self-renewal and, based on indirect evidence, the hypothesis of the existence of a population of resident thyroid stem cells was first put forward by Dumont et al (1992). For example, following the injection of a low number of cells, growth of thyroid transplants indeed occurred in recipient animals (Mulcahi et al 1980). Accordingly, radiobiological cloning experiments demonstrated that foci generation could develop but with very low efficiency.…”

Section: Adult Thyroid Stem/progenitor Cellsmentioning

confidence: 99%

Identifying thyroid stem/progenitor cells: advances and limitations

Fierabracci

2011

Journal of Endocrinology

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Continuing advances in stem cell science have prompted researchers to envisage the potential application of stem cells for the management of several debilitating disorders, thus raising the expectations of transplant clinicians. In particular, in order to find a source of adult stem cells alternative to embryonic stem cells (ESCs) for the exploration of novel strategies in regenerative medicine, researchers have attempted to identify and characterise adult stem/progenitor cells resident in compact organs, since these populations appear to be responsible for physiological tissue renewal and regeneration after injury. In particular, recent studies have also reported evidence for the existence of adult stem/progenitor cell populations in both mouse and human thyroids. Here, I provide a review of published findings about ESC lines capable of generating thyroid follicular cells, thyroid somatic stem cells and cancer stem cells within the thyroid. The three subjects are analysed by also considering the criticism recently raised against their existence and potential utility. I comment specifically on the significance of resident thyroid stem cells in the developmental biology of the gland and their putative role in the pathogenesis of thyroid disorders and on the protocols employed for their identification. I finally provide my opinion on whether from basic science results obtained to date it is possible to extrapolate any convincing basic for future treatment of thyroid disorders.

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Hormonal Effects on the Quantitative Transplantation of Monodispersed Rat Thyroid Cells*

Cited by 14 publications

References 7 publications

Physiological and pathological regulation of thyroid cell proliferation and differentiation by thyrotropin and other factors

Physiological and pathological regulation of thyroid cell proliferation and differentiation by thyrotropin and other factors

On the Cells of Origin of Radiogenic Thyroid Cancer: New Studies Based on an Old Idea

Identifying thyroid stem/progenitor cells: advances and limitations

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