Hormonal control of vas deferens fluid volume and aquaporin expression in rats

Ramli, Norlisah; Giribabu, Nelli; Karim, Kamarulzaman; Salleh, Naguib

doi:10.1007/s10735-018-9804-1

Cited by 8 publications

(10 citation statements)

References 45 publications

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“…These results indicate that the aging of the epididymis and other duct-like structures is closely associated with extracellular matrix changes ( Birch, 2018 ; Romero-Ortuno et al, 2020 ). Considering that the expression profiles of the male reproductive tract can be affected by testosterone and other sex hormones ( Hu et al, 2014 ; Ramli et al, 2019 ), the potential for some of these transcriptome changes may be secondary to the decline in testicular endocrine function cannot be ruled out. Several members of Defensin Beta family ( Defb 18, 19, 20, 39, 40) were both age-related and regulated by orchiectomy and testosterone replacement in mouse caput epididymidis ( Hu et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Anatomical Transcriptome Atlas of the Male Mouse Reproductive System During Aging

Huang

Sun

et al. 2022

Front. Cell Dev. Biol.

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The elderly males undergo degenerative fertility and testicular endocrine function that jeopardize the reproductive health and well-being. However, the mechanisms underlying reproductive aging are unclear. Here, we tried to address this by investigating the phenotypes and transcriptomes of seven regions of the male mouse reproductive tract: the testis, efferent ductules, initial segment, caput, corpus and cauda epididymidis, and vas deferens, in adult (3 months) and aged (21 months) mice. Quantitative PCR, immunohistochemistry, immunofluorescent staining, and enzyme-linked immunosorbent assay were performed for the analysis of gene expression in mice, human tissues, and semen samples. Aged male mice showed both systematic and reproductive changes, and remarkable histological changes were detected in the testis and proximal epididymis. Transcriptomes of the male reproductive tract were mapped, and a series of region-specific genes were identified and validated in mouse and/or human tissues, including Protamine 1 (Prm2), ADAM metallopeptidase domain 28 (Adam28), Ribonuclease A family member 13 (Rnase13), WAP four-disulfide core domain 13 (Wfdc13), and Wfdc9. Meanwhile, age-related transcriptome changes of different regions of the male reproductive tract were characterized. Notably, increased immune response was functionally related to the male reproductive aging, especially the T cell activation. An immune response-associated factor, phospholipase A2 group IID (Pla2g2d), was identified as a potential biomarker for reproductive aging in mice. And the PLA2G2D level in human seminal plasma surged at approximately 35 years of age. Furthermore, we highlighted Protein tyrosine phosphatase receptor type C (Ptprc), Lymphocyte protein tyrosine kinase (Lck), Microtubule associated protein tau (Mapt), and Interferon induced protein with tetratricopeptide repeats 3 (Ifit3) as critical molecules in the aging of initial segment, caput, caput, and cauda epididymidis, respectively. This study provides an RNA-seq resource for the male reproductive system during aging in mice, and is expected to improve our understanding of male reproductive aging and infertility.

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Section: Discussionmentioning

confidence: 99%

Anatomical Transcriptome Atlas of the Male Mouse Reproductive System During Aging

Huang

Sun

et al. 2022

Front. Cell Dev. Biol.

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“…Our high-resolution confocal microscopy images showed well preserved and complete images of stereocilia bundles of individual cells (Figures 4, 5, and 9). Previous studies on the histology of the vas deferens using either histochemical staining or electron microscopic ultrastructure showed mostly cut and scattered stereocilia, for example, Francavilla et al (1987) and Khadijah Ramli et al (2018Ramli et al ( , 2019 that do not represent the dense organization and the structure of the actin bundles.…”

Section: Aqp9 and Cftr Localizationmentioning

confidence: 91%

“…Twelve of the AQP isoforms are expressed in the male and female reproductive systems (Yeste, Morató, Rodríguez-Gil, Bonet, & Prieto-Martínez, 2017;Zhang, Tan, Qu, Sheng, & Huang, 2012). Specifically, the expressions of AQP1, AQP2, AQP7, and AQP9 have been documented in the vas deferens of several species (Andonian & Hermo, 1999;Khadijah Ramli, Giribabu, Karim, & Salleh, 2019;Pastor-Soler et al, 2001;Rojek et al, 2007;Schimming, Pinheiro, de Matteis, Machado, & Domeniconi, 2015;Yeste et al, 2017).…”

mentioning

confidence: 99%

High‐resolution imaging of the actin cytoskeleton and epithelial sodium channel, CFTR, and aquaporin‐9 localization in the vas deferens

Sharma

Kumaran

Hanukoglu

2020

Molecular Reproduction Devel

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Vas deferens is a conduit for sperm and fluid from the epididymis to the urethra. The duct is surrounded by a thick smooth muscle layer. To map the actin cytoskeleton of the duct and its epithelium, we reacted sections of the proximal and distal regions with fluorescent phalloidin. Confocal microscopic imaging showed that the cylinder‐shaped epithelium of the proximal region has a thick apical border of actin filaments that form microvilli. The epithelium of the distal region is covered with tall stereocilia (13–18 µm) that extend from the apical border into the lumen. In both regions, the lateral and basal cell borders showed a thin lining of actin cytoskeleton. The vas deferens epithelium contains various channels to regulate the fluid composition in the lumen. We mapped the localization of the epithelial sodium channel (ENaC), aquaporin‐9 (AQP9), and cystic fibrosis transmembrane conductance regulator (CFTR) in the rat and mouse vas deferens. ENaC and AQP9 immunofluorescence were localized on the luminal surface and stereocilia and also in the basal and smooth muscle layers. CFTR immunofluorescence appeared only on the luminal surface and in smooth muscle layers. The localization of all three channels on the apical surface of the columnar epithelial cells provides clear evidence that these channels are involved concurrently in the regulation of fluid and electrolyte balance in the lumen of the vas deferens. ENaC allows the flow of Na+ ions from the lumen into the cytoplasm, and the osmotic gradient generated provides the driving force for the passive flow of water through AQP channels.

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“…In male rats, APQ9 abundance in epididymal epithelium is modulated by testosterone [ 216 ]. In addition, the rate of ductus deferens fluid secretion under testosterone influence is mediated via the upregulation of AQP1, AQP2 and AQP9 [ 217 ]. In turn, decreased prostatic and seminal vesicle secretions during the ongoing deprivation in androgen level is related to the decrease in AQP0, AQP1, AQP4, AQP5, AQP6 and AQP8 in prostatic tissue and seminal vesicles [ 218 ].…”

Section: Modulation Of Aquaporins By Sex Steroids As An Indirect Mechanism Of Adipose Tissue Regulationmentioning

confidence: 99%

Recent Update on the Molecular Mechanisms of Gonadal Steroids Action in Adipose Tissue

Wawrzkiewicz-Jałowiecka

Lalik

Soveral

2021

IJMS

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The gonadal steroids, including androgens, estrogens and progestogens, are involved in the control of body fat distribution in humans. Nevertheless, not only the size and localization of the fat depots depend on the sex steroids levels, but they can also highly affect the functioning of adipose tissue. Namely, the gonadocorticoids can directly influence insulin signaling, lipid metabolism, fatty acid uptake and adipokine production. They may also alter energy balance and glucose homeostasis in adipocytes in an indirect way, e.g., by changing the expression level of aquaglyceroporins. This work presents the recent advances in understanding the molecular mechanism of how the gonadal steroids influence the functioning of adipose tissue leading to a set of detrimental metabolic consequences. Special attention is given here to highlighting the sexual dimorphism of adipocyte functioning in terms of health and disease. Particularly, we discuss the molecular background of metabolic disturbances occurring in consequence of hormonal imbalance which is characteristic of some common endocrinopathies such as the polycystic ovary syndrome. From this perspective, we highlight the potential drug targets and the active substances which can be used in personalized sex-specific management of metabolic diseases, in accord with the patient’s hormonal status.

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Hormonal control of vas deferens fluid volume and aquaporin expression in rats

Cited by 8 publications

References 45 publications

Anatomical Transcriptome Atlas of the Male Mouse Reproductive System During Aging

Anatomical Transcriptome Atlas of the Male Mouse Reproductive System During Aging

High‐resolution imaging of the actin cytoskeleton and epithelial sodium channel, CFTR, and aquaporin‐9 localization in the vas deferens

Recent Update on the Molecular Mechanisms of Gonadal Steroids Action in Adipose Tissue

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