Hormonal control of uteroglobin secretion in rabbit uterus. Inhibition of uteroglobin synthesis and messenger ribonucleic acid accumulation by oestrogen and anti-oestrogen administration

Abstract

Investigations were conducted to quantify activity of uteroglobin mRNA and secretion of uteroglobin in rabbit uterus after administration of progesterone and 5alpha-dihydrotestosterone, either alone or concomitantly with oestradiol-17beta and tamoxifen, a non-steroidal anti-oestrogen. Poly(A)-containing mRNA was isolated from the uterine tissue by extraction with phenol/chloroform, precipitation with ethanol and chromatography on oligo(dT)-cellulose. Cell-free translation in vitro of the poly(A)-containing mRNA was carried out in a wheat-germ lysate, and the product isolated by specific immuno-precipitation with anti-uteroglobin antiserum purified by affinity chromatography. Radioimmunoassay was utilized to determine uteroglobin content in the uterine flushings and tissue preparations. When given for 5 days, both progesterone (1mg/kg per day) and 5alpha-dihydrotestosterone (25mg/kg per day) elicited a marked induction of uteroglobin secretion, which was accompanied with accumulation of uteroglobin mRNA in the tissue. Concomitant administration of oestradiol-17beta (50mug/kg per day) or tamoxifen (12.5mg/kg per day) significantly decreased both progesterone- and 5alpha-dihydrotestosterone-induced uteroglobin secretion, with a parallel decrease in the uteroglobin-mRNA activity. The decline in the uteroglobin content of the uterine flushes brought about by oestradiol-17beta or tamoxifen administration was not due to inhibition of secretion of this protein by the endometrial cells, since a simultaneous decrease occurred in the tissue uteroglobin content. After a 5-day pretreatment with progesterone (1mg/kg per day), administration of oestradiol-17beta (50mug/kg per day) during the ensuing 4 days greatly accelerated the decay of the uteroglobin content in the uterine fluid.

Oviductal and endometrial mRNA expression of implantation candidate biomarkers during early pregnancy in rabbit

“…In rabbit, uteroglobin ( SCGB1A1 ) comprises 40–60% of the total protein from the histotroph uterine secretion on day 5 of pregnancy. These high levels observed close to early events of implantation (Krishnan & Daniel, 1967; Beier 1968) are induced by the progressive increase of progesterone levels together with the decreasing levels of oestrogens in this period (Kopu et al ., 1979; Chandra et al ., 1980; Muller & Beato, 1980; Snead et al ., 1981, Shen et al ., 1983).…”

A novel progesterone-induced messenger RNA in rabbit and human endometria. Cloning and sequence analysis of the complementary DNA

“…Uteroglobin (Beier, 1968) or blastokinin (Krishnan & Daniel, 1967) is the major secretory protein of the progesteronestimulated endometrium. Its gene has been isolated (Atger et al, 1981;Snead et al, 1981; Suske et al, 1983), and many features of its hormonal regulation have been unraveled 0006-2960/87/0426-3975801.50/0 © 1987 American Chemical Society ( Torkkeli et al, 1977;Kopu et al, 1979;Savouret et al, 1980;Loosfelt et al, 1981;Heins & Beato, 1981;Kumar et al, 1982;Bailly et al, 1983). However, progesterone provokes a wide range of modifications in the endometrium, many of which are necessary for implantation of the blastocyst.…”

Progesterone Action in Mammalian Uterus

“…Interestingly, these latter times cover the lag period in uteroglobin induction, and the most rapid period of progesterone receptor processing or down-regulation (24, 50, 51). Moreover, when estradiol is administered to animals chronically exposed to progesterone, a dramatic acceleration in the decay of uteroglobin and its mRNA content takes place (27,34).…”