Hormonal Control of Sucrose Phosphate Synthase and Sucrose Synthase in Sugar Beet

Sakalo, V. D.; Kurchii, V. M.

doi:10.1023/b:rupp.0000019211.97216.8d

Cited by 8 publications

(4 citation statements)

References 15 publications

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“…In agreement, Tateishi et al (2004) stated that accumulation of sucrose during maturation of kiwi fruits was coinciding with an increase in SPS activity. In this study; results of foliar SPS activity altogether with the total chlorophyll content decreased at the last harvest that coincides with previous finding by Sakalo & Kurchii (2004).…”

Section: Discussionsupporting

confidence: 93%

Key Regulators of Sucrose Accumulation during Different Developmental Phases of Sugar Beet Plants

Ali

2018

Egypt. J. Bot.

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B ECAUSE of the economic importance of sugar beet (Beta vulgaris L) in sucrose production, a field experiment was conducted to elucidate the key regulators of sucrose metabolism in source leaves and sink roots at 135, 150, 165 and 180 day after sowing (DAS) that would be determined sucrose yield. Increase in sucrose phosphate synthase (SPS) activity in source and sink tissues during the first three selected harvest phases was associated in leaves with an increase in activities of acid invertase (AI) and neutral invertase (NI), chlorophyll a, total chlorophyll and reducing sugars on the one hand and the levels of sucrose and inorganic phosphorus (P i ) in roots on the other hand. The maximum sucrose level in roots was at 165 DAS. A gradual increase in root NI activity accompanied by an increase in reducing sugars and a decrease in the sucrose/reducing sugars ratio in roots by harvest date progress was observed. Pearson correlation test revealed contradictory findings whereas, SPS activity correlated with sucrose level in leaves negatively but positively in roots. All investigated enzymes in leaves correlated negatively with sucrose/reducing ratio. In roots, only NI activity correlated negatively with sucrose/reducing sugars ratio.

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Section: Discussionsupporting

confidence: 93%

Key Regulators of Sucrose Accumulation during Different Developmental Phases of Sugar Beet Plants

Ali

2018

Egypt. J. Bot.

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“…Slowing protein degradation is a necessary condition for a high level of cellular metabolism. Protein synthesis and increased enzyme activity are closely interrelated [5]. According to our data, the increased enzyme activity (Table 1) caused by D-NAA in L-70 correlated with increased protein content.…”

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confidence: 47%

Effect of α-naphthylacetic and gibberellic acids on inhibitor protein content and fiber-forming enzyme activity of gymnosperm and fuzzy cotton

et al. 2010

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The effect of the phytohormones D-naphthylacetic acid and gibberellic acid on the protein content; glucansynthetase, peroxidase, and cellulase enzyme activity; and development of gymnosperm and fuzzy cotton ovules was studied. The effect on cellulose synthesis of an inhibitor protein isolated from integument of gymnosperm cotton treated with gibberellic acid was investigated.Phytohormones have developed through evolution and are a genetically stable system of compounds that regulate the activity of the genetic apparatus during growth processes and senescence and protect the whole plant or its separate organs from the effects of extreme environmental factors and microflora [1]. The phytohormonal system is a product of the genotype and has a large inductor or repressor influence on the activity of certain genes or their blocks. All this eventually affects the quantity and quality of the harvest.The most important plant hormones are auxins and gibberellins. Only a few reports on the hormonal regulation of cotton cellulose synthesis have appeared. These mainly analyze the levels of endogenous hormones in cells of cotton seed fibers and not features of the exogenous action of phytohormones [2]. The phytohormones D-naphthylacetic acid (D-NAA) and gibberellic acid (GA) have great significance for increasing cellulose fiber formation [3].We investigated the action of phytohormones D-NAA and GA on protein content, ovule development, and glucansynthetase (GS), peroxidase (PO), and cellulase (C) enzyme activity in 20-day integument of gymnosperm (line L-70) and fuzzy (variety AN-Bayaut-2) cotton.According to the results, phytohormone D-NAA had no effect on the total protein content in AN-Bayaut-2 whereas it increased it in line L-70 by 13% (Table 1). GA increased the amount of protein by 35% in AN-Bayaut-2 and by 6% in line L-70. It has been hypothesized that D-NAA and GA activate repressor genes by interacting with low-molecular-weight repressor proteins, thereby inducing the synthesis of RNA molecules. This in turn leads to the synthesis of new types of enzymes or enhances the synthesis of already existing enzymes [1].An electrophoretic study of total proteins from integuments of control plants and those treated with gibberellin and D-NAA showed that distinctive proteins with various molecular weights were present in addition to a large number of minor protein components (Fig. 1). The color intensity of proteins from samples treated with GA increased in fuzzy variety AN-Bayaut-2.This indicated that the protein concentration increased in all ovule samples. The increased protein concentration caused by gibberellin may have been due to functional specifics of this phytohormone.It has been reported [4] that the growth period increased upon treatment of plants with GA before flowering. In other words, it slowed the flowering process itself. However, treatment of the plant with this same phytohormone during flowering accelerated the fruit ripening process, because of which it can be concluded that the biosynthesis of proteins w...

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“…Активність проліндегідрогенази оцінювали за швидкістю використання НАД + на окислення L-проліну, шляхом вимірювання збільшення концентрації НАДН за одиницю часу (1 хв) [10]. Вміст розчинних вуглеводів визначали резорциновим методом після фіксації рослинного матеріалу киплячим етанолом та триразового екстрагування цукрів 80 %-м етанолом [11].…”

Section: матеріали і методиunclassified

Physiological and biochemical characteristics of genetically modified winter wheat

Mykhalska,

Komisarenko,

Kurchii

et al. 2023

Fakt. eksp. evol. org.

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Aim. Analysis of physiological and biochemical characteristics of genetically modified wheat with partially suppressed activity of the proline dehydrogenase gene. Methods. Determination of proline dehydrogenase (PDH) activity, free L-proline (Pro); carbohydrate content (sucrose and fructose). Results. It was established that under conditions of water deficit in genetically modified wheat plants with integrated elements forming the double-stranded RNA suppressor of the pdh gene, there is a partial inhibition of the enzyme activity and an increase in the content of free proline. Changes in carbohydrate metabolism under water deficit stress and in the first hours after rehydration were noted, while the sucrose / fructose ratio in control plants significantly decreases during dehydration and normalizes when water supply is restored, in genetically modified plants this indicator almost does not change under short-term water deficit stress. Conclusions. Partial suppression of the proline dehydrogenase gene leads to an increase in the free proline content, the fluctuations of which contribute to the maintenance of carbohydrate balance.

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Hormonal Control of Sucrose Phosphate Synthase and Sucrose Synthase in Sugar Beet

Cited by 8 publications

References 15 publications

Key Regulators of Sucrose Accumulation during Different Developmental Phases of Sugar Beet Plants

Key Regulators of Sucrose Accumulation during Different Developmental Phases of Sugar Beet Plants

Effect of α-naphthylacetic and gibberellic acids on inhibitor protein content and fiber-forming enzyme activity of gymnosperm and fuzzy cotton

Physiological and biochemical characteristics of genetically modified winter wheat

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