Hormonal control of proliferation in meristematic agglomerates of Eucalyptus camaldulensis Dehn

Arezki, Ouoimare; Boxus, P.; Kevers, Claire; Gaspar, Thomas

doi:10.1007/s11627-000-0071-x

Cited by 15 publications

(5 citation statements)

References 21 publications

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“…This is in accordance with results of Arezi et al (2000), who analysed endogenous levels of free polyamines and concluded that these substances had no significant physiological effect on the in vitro formation of E. camaldulensis meristematic aggregates. This is in accordance with results of Arezi et al (2000), who analysed endogenous levels of free polyamines and concluded that these substances had no significant physiological effect on the in vitro formation of E. camaldulensis meristematic aggregates.…”

Section: Discussionsupporting

confidence: 92%

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Meristematic nodule culture: a new pathway for in vitro propagation of Eucalyptus globulus

Trindade

Pais

2003

Trees

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Morphogenesis was induced in Eucalyptus globulus seeds, cotyledons, hypocotyls and leaves from in vitro clonal plantlets. Globular structures were observed after 2 weeks induction on B5 culture medium supplemented with 10% coconut water, 0.05-0.5 mg l 1 6-benzylaminopurine (BAP) and 0.5 mg l 1 indole-3butyric acid (IBA). These continued to proliferate under dark conditions until the 2nd to 3rd subculture. Following transfer to a photoperiod of 16 h light, shoots evolved from these globular structures and developed further to plantlets. The influence of several factors, including culture medium composition, sucrose concentration, the type, concentration and combination of growth regulators and the presence of coconut water was studied. The percentage of explants showing globular structure formation and the number of globular structures per explant were evaluated. Macroscopic, histological and scanning electron microscopic studies revealed that the morphogenic process involved mainly organogenic nodules with fewer globular somatic embryos. The nodules gave rise to shoots and subsequently complete plants following incubation on B5 Gamborg medium containing 0.5 mg l 1 IBA and 30 g l 1 sucrose, which promoted root formation.

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Section: Discussionsupporting

confidence: 92%

“…In Eucalyptus, the development of an embryogenic system has proved difficult. Recently, the formation of E. camaldulensis meristematic agglomerates was reported by Arezi et al (2000). None of these methods was developed for E. globulus.…”

Section: Introductionmentioning

confidence: 99%

Meristematic nodule culture: a new pathway for in vitro propagation of Eucalyptus globulus

Trindade

Pais

2003

Trees

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“…Micropropagation may be a valuable alternative when: (a) conventional propagation is difficult to achieve, (b) problems of rejuvenation persist, and (c) pressure to increase multiplication rates occurs (McComb and Bennett 1986;Gomes and Canhoto 2003). Several micropropagation techniques such as somatic embryogenesis (Canhoto et al 1999), organogenesis (Arezki et al 2000), and axillary shoot proliferation (several authors in Jain and Häggman 2007) have been applied for in vitro propagation of woody plants. Among these methods, axillary shoot proliferation is the most widely used for Ericaceae clonal propagation.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of strawberry tree (Arbutus unedo L.) from adult plants

Gomes

Canhoto

2008

In Vitro Cell.Dev.Biol.-Plant

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Arbutus unedo L. is a species of strawberry tree, widely represented in the Mediterranean climates of southern Europe. Fruits are used to make jellies and a spirit called "medronheira." Shoot apices and nodal segments from epicormic and coppiced shoots of adult plants were used for plant propagation. Shoot apices from epicormic shoots, which were developed in a growth chamber, showed higher rates of in vitro establishment. The results also indicated that shoot apices are more effective for plant establishment than nodal segments, with rates of establishment significantly higher after 12 wk of culture. Of the three basal media used in combination with 9.0 µM benzyladenine and 0.087 M sucrose, the FS medium with the micronutrients of the Murashige and Skoog medium gave the highest rates of multiplication, especially when the parameter analyzed was the number of clusters formed. When shoot apices from selected adult plants (AL01-AL06) were tested, the multiplication rate was not significantly different among the plants. However, in the conditions tested, shoots from the clones AL1, AL2, and AL3 showed better development, whereas shoots from AL4, AL5, and AL6 showed an impaired development and could not be rooted. Rooting was achieved in all the conditions tested, even in the absence of auxin. The inclusion of an auxin significantly increased root formation, whereas the addition of charcoal did not improve root formation. Rooted plantlets were acclimatized, and some of them are now in the field for further study.

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“…The culture medium used was an adaptation of Muralidharan & Mascarenhas (1987) medium, supplemented with 1.0 g L -1 casein, 16.2 µmol L -1 NAA, 0.44 µmol L -1 BAP, 50 g L -1 (w/v) sucrose and 0.5% (w/v) Phytagar ® . Arezki et al (2000) observed the proliferation of meristematic agglomerates in E. camaldulensis explants cultured on MAS culture medium [MS medium containing 5 µmol L -1 indolebutyric acid, 30 g L -1 (w/v) sucrose, 0.5% (w/v) agar and 0.2% (w/v) activated charcoal]. Diallo & Duhoux (1984) obtained plant regeneration from cotyledonary leaves of the same species using MS medium supplemented with NAA, BAP and 20 g L -1 (w/v) sucrose.…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis

Dibax¹,

Eisfeld

Cuquel³

et al. 2005

Sci. agric. (Piracicaba, Braz.)

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Breeding methods based on genetic transformation techniques need to be implemented for Eucalyptus camaldulensis to shorten the long breeding cycles and avoid manipulation of adult trees; that requires the development of plant regeneration protocols enabling development of plants from transformed tissues. The present work aimed to optimise the regeneration process already established for the species. Cotyledonary leaves of E. camaldulensis were cultured in MS medium supplemented with naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) combinations. The most efficient treatment for bud indirect regeneration (2.7 µmol L -1 NAA and 4.44 µmol L -1 BAP) was used for further experiments. When explants were kept in the dark during the first 30 days, the percentage of explants forming calluses increased and explant necrosis was reduced in comparison with light-cultured explants. Mineral medium modifications were compared and half-strength MS mineral medium turned out to be as efficient as full-strength medium, producing 54% and 47% of explants with buds, respectively. For shoot elongation, MS medium with halfstrength nitrate and ammonium salts, and 0.2% activated charcoal yielded rooted shoots 1 to 8 cm high after one month. The procedure is an efficient protocol for E. camadulensis plant regeneration, reducing the stages necessary for the obtention of complete plants. Key words: in vitro culture, forest species, culture media, organogenesis REGENERAÇÃO DE PLANTAS DE Eucalyptus camaldulensis A PARTIR DAS EXPLANTES COTILEDONARESRESUMO: A implementação, para espécies florestais, de técnicas de melhoramento baseadas em métodos de transformação genética, permitirá reduzir os longos ciclos de melhoramento e evitar a manipulação de árvores adultas. Isto implica dispor de um protocolo de regeneração que permita o desenvolvimento de plantas a partir de tecidos transformados. Este trabalho teve como objetivo otimizar este protocolo de regeneração para Eucalyptus camaldulensis. Folhas cotiledonares foram cultivadas em meio de cultura MS suplementado com combinações de ácido naftalenoacético (ANA) e 6-benzilaminopurina (BAP). O tratamento mais eficiente em termos de regeneração indireta de gemas foi 2,7 µmol L -1 de ANA combinado com 4,44 µmol L -1 de BAP, o qual foi utilizado nos experimentos posteriores. A manutenção dos explantes no escuro durante os trinta primeiros dias elevou a porcentagem de explantes com calos e reduziu a morte dos explantes, em comparação com os que permaneceram na luz. Modificações da composição mineral do meio MS foram comparadas e mostraram que a redução de metade dos sais foi tão eficiente para a formação de gemas (54% dos explantes) quanto o meio completo (47%). O meio de cultura com a concentração de íons nitrato e amônio reduzida à metade e 0,2% de carvão ativado apresentou-se adequado para o alongamento e enraizamento das brotações que atingiram uma altura de 1 a 8 cm depois de 30 dias. O processo completo representa um protocolo eficiente para a regeneração de plantas de Eucalyptu...

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Hormonal control of proliferation in meristematic agglomerates of Eucalyptus camaldulensis Dehn

Cited by 15 publications

References 21 publications

Meristematic nodule culture: a new pathway for in vitro propagation of Eucalyptus globulus

Meristematic nodule culture: a new pathway for in vitro propagation of Eucalyptus globulus

Micropropagation of strawberry tree (Arbutus unedo L.) from adult plants

Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis

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