Hormonal and molecular characterization of calcium oxalate stone formers predicting occurrence and recurrence

Elshal, Ahmed M.; Shamshoun, Heba; Awadalla, Amira; Elbaz, Rizk; Ahmed, Asmaa; El-Khawaga, Omali Y.; Shokeir, Ahmed A.

doi:10.21203/rs.3.rs-2147324/v1

Cited by 3 publications

(4 citation statements)

References 15 publications

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“…One examination method that is often used for urine examination is by using microscopic methods. Microscopic examination of urine requires a sample that matches the needs of the examination (Elshal et al, 2023). The factors that affect urine results are time, temperature, storage container.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Urine Storage for 24 hours on Results of Urinary Leukocytes and Calcium Oxalate

Elfrida

2024

Int. J. Multidiscipline. Approach. Res. Sci.

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Urine examination is an examination often used and is most commonly done to help establish the diagnosis of a disease. The urine test consists of macroscopic, microscopic examination, and chemical examination of urine. Microscopic tests to look at erythrocytes, leukocytes, epithelial cells, thorax, bacteria, crystals, fungi and parasites. Objective: To determine the effect of urine storage for 24 hours on the results of urinary leukocytes and calcium oxalate in Binawan University staff. Method: This type of research uses primary data with a quantitative descriptive design with an analytical observation approach. This research data uses SPSS Software. This data processing is done descriptively. The tests used were the Kruskal-Wallis test and the Mann-Whitney Test Results: The frequency distribution of the description of fresh urine leukocyte results was the most 1-5 / LPB with a frequency of 26 people with a percentage of 86.7%. The distribution of the frequency of 24-hour delayed urine results was mostly 1-5 / LPB with a frequency of 29 people with a percentage of 96.7%. The distribution of fresh urine calcium oxalate was the most 1-5 / LPB with a frequency of 23 people with a percentage of 76.7%., and the frequency distribution of 24-hour delayed urine calcium oxalate results was at most 0 / LPB as many as 20 people with a percentage of 66.7%. Conclusion: In leukocyte examination, fresh urine calcium oxalate and delayed 24 hours obtained a p value of 0.00 and the results can be concluded that there is an effect of urine storage for 24 hours on the results of leukocytes and calcium oxalate urine.

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Section: Discussionmentioning

confidence: 99%

The Effect of Urine Storage for 24 hours on Results of Urinary Leukocytes and Calcium Oxalate

Elfrida

2024

Int. J. Multidiscipline. Approach. Res. Sci.

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“…1,10 Contrary to our findings, female predominance has been reported by Koirala et al 11 A new trend with more female involvement has been noted over near past that may be attributed to change in lifestyle. 12,13 Out of 100 samples, bacterial growth on culture was obtained from the urine samples of 46 patients. Worldwide, varied prevalence both higher and lower has been reported.…”

Section: Discussionmentioning

confidence: 99%

“…Worldwide, varied prevalence both higher and lower has been reported. [10][11][12][13] This variation is because of different antibiotic prescription patterns in various countries, and varying susceptibility toward antibiotics in different population. The most common isolated organisms were E. coli, Klebsiella sp., followed by others such Proteus sp., Enterococcus sp., Staphylococcus sp., and Pseudomonas aeruginosa.…”

Section: Discussionmentioning

confidence: 99%

Microbiological analysis of urinary calculi: A study from a tertiary care hospital of Eastern India

Kumar,

Kunal,

Manish Kumar

et al. 2023

Asian J Med Sci

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Background: Among various renal pathology, urolithiasis among patients of urinary tract infections (UTIs) forms a major group. The pathophysiology of such patients varies from the group of urolithiasis patients without infection. Aims and Objectives: The current study was planned and conducted to find out the bacteriological profile of urinary calculi among patients of UTI. Materials and Methods: A descriptive study was conducted by the Department of Microbiology and Surgery of JLNMC, Bhagalpur, Bihar. A total of 100 patients were enrolled in the study, having urinary calculi who either visited the outpatient department or who were admitted at the hospital for planned procedure for removal of stone between January 2021 and December 2021. Results: Male predominance was noted with a mean age of presentation at 44.3 years. 67% of the patients had some or the other symptoms of UTI, most common symptom being excruciating groin pain. Recurrent episodes of UTI were noted among female patients. Bacterial growth on culture was obtained from the urine samples of 46 patients. Escherichia coli were the most common isolated organism. Most common type of stone was a mixed type of stone formed by calcium oxalate and calcium phosphate followed by struvite stones and uric acid stones. Conclusion: Various factors of clinical significance among patients of urolithiasis and UTI are gender, age, and clinical symptoms. Gram-negative bacilli are the most common bacteria found in UTIs in patients with urolithiasis.

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“…Calcium oxalate stones are the most common type of kidney stone, accounting for up to 75% of all kidney stones formed. Oxalate is a natural substance found in many foods, and when there is too much oxalate in the urine, crystals can form and stick together to form a solid mass (3).…”

Section: Introductionmentioning

confidence: 99%

Optimizing Antigen Preparation for Oxalyl-CoA Decarboxylase Enzyme Diagnostic Kit and ELISA System Cutoff Determination

Ardestani,

Basiri,

Bandehpour

et al. 2024

Preprint

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The prevalence of kidney stone disease is increasing globally, with calcium oxalate stones being the most common type. Oxalyl-CoA decarboxylase (OXC), an enzyme produced by the gut bacterium Oxalobacter formigenes, plays a crucial role in oxalate metabolism. Deficiencies in OXC activity can lead to the accumulation of oxalate, contributing to kidney stone formation. This study aimed to develop a reliable diagnostic assay for OXC by optimizing antigen production and establishing a cutoff value for an enzyme-linked immunosorbent assay (ELISA). We cloned, expressed, and purified recombinant OXC protein in Escherichia coli BL21(DE3), and generated specific polyclonal antibodies in rabbits. The ELISA system was optimized and validated using serum samples from 40 healthy individuals and 6 patients with oxalate-related disorders. The cutoff value was determined using the formula ( M + 2SD ), where ( M ) is the mean and ( SD ) is the standard deviation of the healthy sample results. The calculated cutoff value of 0.656750 effectively distinguished between healthy and affected individuals, with a sensitivity of 97.5% and a specificity of 83.3%. These findings provide a valuable tool for the early detection and management of oxalate-related disorders, with significant implications for clinical practice.

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Hormonal and molecular characterization of calcium oxalate stone formers predicting occurrence and recurrence

Cited by 3 publications

References 15 publications

The Effect of Urine Storage for 24 hours on Results of Urinary Leukocytes and Calcium Oxalate

The Effect of Urine Storage for 24 hours on Results of Urinary Leukocytes and Calcium Oxalate

Microbiological analysis of urinary calculi: A study from a tertiary care hospital of Eastern India

Optimizing Antigen Preparation for Oxalyl-CoA Decarboxylase Enzyme Diagnostic Kit and ELISA System Cutoff Determination

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