Hormesis response of marine and freshwater luminescent bacteria to metal exposure

Shen, Ke; Shen, Chaofeng; Liu, Yuan; Tang, Xianjin; Zhang, Congkai; Chen, Xincai; Shi, Jiyan; Qiu, Lin; Chen, Yingxu

doi:10.4067/s0716-97602009000200006

Cited by 37 publications

(20 citation statements)

References 13 publications

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“…Marine and freshwater luminescent bacteria exposed to metals, showed a clear hormetic phenomenon in luminescence assays (Christofi et al 2002;Shen et al 2009;Deng et al 2012). However, in pathogenic and commensal bacteria the occurrence of hormetic response induced by low doses of antibiotics has not been yet demonstrated.…”

Section: Introductionmentioning

confidence: 99%

Low Doses of Tetracycline Trigger the E. coli Growth: A Case of Hormetic Response

Rotini¹,

Thaller²

2013

Dose-Response

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ᮀ Hormesis is a biphasic dose-response relationship, occurring when low concentrations of toxic agents elicit apparent improvements. In this work, the ability of sub-inhibitory concentrations of Tetracycline to induce hormetic response in a model organism was investigated. To this aim a reference strain of Escherichia coli, MG1655, was exposed to six decreasing doses of Tetracycline (between 0.12 and 0.00375 μg/ml), much lower than the Minimal Inhibitory Concentration (4 μg/ml). An hormetic increase was observed at the intermediate concentrations (0.015-0.03 μg/ml) of the tested range. The Colony Forming Unit number, indeed, rose up to 141% and 121% as compared to the control. At the highest (0.12 μg/ml) and lowest (0.00375 μg/ml) concentrations a slight decrease in CFU number was found. Results demonstrated that, in Escherichia coli, low concentrations of Tetracycline bias the bacterial numerical increase through a hormetic response; the doseresponse curve describing this numerical increase is an U-inverted curve. Furthermore, these data confirm that hormesis is common to many -if not all -living systems, including bacteria; they underline the relevance of a deepened knowledge of both the effects and the possible consequences of exposure to low doses of contaminants.

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Section: Introductionmentioning

confidence: 99%

Low Doses of Tetracycline Trigger the E. coli Growth: A Case of Hormetic Response

Rotini¹,

Thaller²

2013

Dose-Response

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“…Samples showing hormesis are currently non-toxic. Hormesis is a widespread phenomenon in the exposure to metals with luminescent bacteria, because the V. fischeri is generally less sensitive to metals than to plant and animal cells [47,48]. In the research by Shen et al [48], a clear hormesis phenomenon was observed in all tested metals (Cu, Zn, Cd, Cr) at a low concentration under the condition of luminescence assay.…”

Section: Vibrio Fischeri Responsementioning

confidence: 99%

“…Hormesis is a widespread phenomenon in the exposure to metals with luminescent bacteria, because the V. fischeri is generally less sensitive to metals than to plant and animal cells [47,48]. In the research by Shen et al [48], a clear hormesis phenomenon was observed in all tested metals (Cu, Zn, Cd, Cr) at a low concentration under the condition of luminescence assay. Luminescence stimulation by exposure to zinc, cadmium, and chromium was also found in research by Christofi et al [43], who showed an enhancement of luminescence induced by low concentrations of these metals.…”

Section: Vibrio Fischeri Responsementioning

confidence: 99%

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Impact of thermal treatment of mixtures of sewage sludge and plant material on selected chemical properties and Vibrio fischeri response

Gondek

Baran

Mierzwa–Hersztek

et al. 2017

Ecological Chemistry and Engineering S

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Abstract:The aim of the research was to evaluate the effect of temperature during the treatment process as well as the effect of adding plant materials to sewage sludge on selected chemical properties and Vibrio fischeri response. The mixtures were placed in a chamber furnace, under airless conditions. Two temperature procedures were applied: 300 and 600ºC; the exposure time in both cases was 15 minutes. Thermal treatment of sewage sludge without a plant component is not well-founded and may cause an increase in concentration of trace elements. Using the temperature of 300ºC caused significantly lower changes in the contents of total forms of trace elements than using the temperature of 600ºC. The metals extracted from the studied mixtures were not toxic for the Vibrio fischeri. In the case of the fractional composition of humic compounds, thermal treatment of mixtures of sewage sludge and plant materials is not beneficial in terms of labile fractions, but it had a beneficial effect on stabilizing the durable bonds between C compounds in those mixtures.

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“…This is called as hormesis that somehow the bacteria's viability gets stronger than the initial state. Shen et al (2009) reported the stimulatory effect of low concentrations of toxic chemicals on organismal metabolism is common in luminescent bioassay (Fulladosa et al, 2005(Fulladosa et al, , 2007. The increased light amount varies at exposure time and dose of toxicant.…”

Section: Hormesismentioning

confidence: 99%

Proposed method for controlling turbid particles in solid-phase bioluminescent toxicity measurement

Yeo

Park

Ahn

et al. 2015

Environ Monit Assess

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In the recent half century, numerous methods have been developed to assess ecological toxicity. However, the presence of solid-particle turbidity sometimes causes such tests to end with questionable results. Many researchers focused on controlling this arbitrary turbidity effect when using the Microtox® Solid-Phase toxicity system, but there is not yet a standard method. In this study, I examined four solid-phase sample test methods recommended in the Microtox® manual, or proposed from the literature, and compared the existing methods with the proposed method (c-BSPT, centrifuged-BSPT). Four existing methods use the following strategies to control turbid particles: complete separation of liquid and solid using 0.45-μm filtration before contacting solid samples and bacteria, natural settlement, moderate separation of large particles using coarser-pore-size filtration, and exclusion of light loss in the toxicity calculation caused by turbidity after full disturbance of samples. The proposed method uses moderate centrifugation to separate out the heavier soil particles from the lighter bacteria after direct ii contact between them. Among the solid-phase methods tested, in which the bacteria and solid particles were in direct contact (i.e., three existing methods and the newly proposed one, c-BSPT), no single method could be recommended as optimal for samples over a range of turbidity. Instead, a simple screening strategy for selecting a sample-dependent solid-phase test method was suggested, depending on the turbidity of the solid suspension.The results of this study highlight the importance of considering solid particles, and the necessity for optimal selection of test method to reduce errors in the measurement of solid-phase toxicity.

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Hormesis response of marine and freshwater luminescent bacteria to metal exposure

Cited by 37 publications

References 13 publications

Low Doses of Tetracycline Trigger the E. coli Growth: A Case of Hormetic Response

Low Doses of Tetracycline Trigger the E. coli Growth: A Case of Hormetic Response

Impact of thermal treatment of mixtures of sewage sludge and plant material on selected chemical properties and Vibrio fischeri response

Proposed method for controlling turbid particles in solid-phase bioluminescent toxicity measurement

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