Hormesis of some organic solvents on Vibrio qinghaiensis sp.-Q67 from first binding to the β subunit of luciferase

Abstract

Hormesis is a biphasic concentration–response relationship. During the luminescence inhibition test ofVibrio qinghaiensissp.-Q67 (Q67), some organic solvents display the hormesis phenomenon.

Protein Model and Function Analysis in Quorum-Sensing Pathway of Vibrio qinghaiensis sp.-Q67

“…[35][36][37][38][39][40][41]. It is worth noting that Q67 has also exhibited hormetic phenomena in ionic liquids [42,43], sodium halide salts [44], organic solvent [45], and commercial personal care products [46].…”

“…The crystal structure of FMN, the product of the reaction, bound to V. harveyi luciferase has been reported, while the crystals of the luciferase/FMNH 2 complex degraded rapidly and were unsuitable for data collection [90]. In Q67, FMN may bind to the active site pocket formed by eight β-sheets (β1, β2, β3, β5, β8, β9, β10, and β13) in the α-subunit (Figure 5(a-3,a-4)), and there is a similar pocket in the β-subunit (Figure 5(a-3)), which may bind other small molecules to affect the activity of the whole enzyme, such as hermetic organic solvents [45]. However, no structural information on the spatial orientation of the luciferase with the substrate (FMNH 2 and the aldehyde) or intermediates of the reaction is available, preventing a more detailed interpretation of how the overall reaction is generated.…”

“…[35][36][37][38][39][40][41]. It is worth noting that Q67 has also exhibited hormetic phenomena in ionic liquids [42,43], sodium halide salts [44], organic solvent [45], and commercial personal care products [46]. Since the luminescence inhibition rate for the luminescent bacteria is used as the endpoint in toxicity assessment, it is important to study the mechanism of bioluminescence, with the further study of Q67.…”

Time-dependent synergism of five-component mixture systems of aminoglycoside antibiotics toVibrio qinghaiensissp.-Q67 induced by a key component

“…The APTox program was used to t concentration-response curves (CRCs), calculate the median effective concentrations (EC 50 ) and the 95% condence intervals in different exposure time. 22 The specic tting functions are Logit (2) and Weibull (3), the expressions are as follows: 22,28 where E represents the effect (0 # E # 1), c represents the concentration of a single compound or mixture, a and b are model parameters.…”

“…[22][23][24] The inhibition data of single antibiotic and its vecomponent mixtures to V. qinghaiensis at different exposure time (0.25, 2, 4, 8, and 12 h) were determined by timedependent microplate toxicity analysis method. [25][26][27] The concentration-effect data were tted by nonlinear least square method, the toxicity interaction of mixture systems was analysed by concentration addition model (CA) with 95% condence interval, [17][18][19][20][21][22]28 and the degree of toxicity interaction was characterized by deviation from CA model (dCA). Besides, the luminescence inhibition mechanism of ve antibiotics and their ve-component mixtures to V. qinghaiensis was preliminarily determined by observing the cell morphology.…”