Horizontal Transmission of Microbial Symbionts Within a Guild of Fly Parasitoids

Tzuri, Noam; Caspi-Fluger, Ayelet; Betelman, Kfir; Shalom, Sarit Rohkin; Chiel, Elad

doi:10.1007/s00248-020-01618-2

Cited by 8 publications

(10 citation statements)

References 51 publications

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“…It is also possible that additional insect species feeding on the same host plants might take up microorganisms transferred by the bug to the plant. Rickettsia have been shown to be acquired from environmental sources by two species of Spalangia (Hymenoptera: Pteromalidae), S. endius and S. cameroni (Tzuri et al, 2021). Furthermore, Chrostek et al ( 2017 In M. melanotoma, R. bellii is clustered with the bellii group based on the CoxA and GltA genes, but is more similar to the R. limoniae group according to the 16S rRNA gene phylogeny.…”

Section: Discussionmentioning

confidence: 99%

Rickettsia association with two Macrolophus (Heteroptera: Miridae) species: A comparative study of phylogenies and within-host localization patterns

Dally

Izraeli²,

Belausov³

et al. 2023

Front. Microbiol.

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Many arthropods host bacterial symbionts, some of which are known to influence host nutrition and diet breadth. Omnivorous bugs of the genus Macrolophus (Heteroptera: Miridae) are mainly predatory, but may also feed on plants. The species M. pygmaeus and M. melanotoma (=M. caliginosus) are key natural enemies of various economically important agricultural pests, and are known to harbor two Rickettsia species, R. bellii and R. limoniae. To test for possible involvement of symbiotic bacteria in the nutritional ecology of these biocontrol agents, the abundance, phylogeny, and distribution patterns of the two Rickettsia species in M. pygmaeus and M. melanotoma were studied. Both of the Rickettsia species were found in 100 and 84% of all tested individuals of M. pygmaeus and M. melanotoma, respectively. Phylogenetic analysis showed that a co-evolutionary process between Macrolophus species and their Rickettsia is infrequent. Localization of R. bellii and R. limoniae has been detected in both female and male of M. pygmaeus and M. melanotoma. FISH analysis of female gonads revealed the presence of both Rickettsia species in the germarium of both bug species. Each of the two Rickettsia species displayed a unique distribution pattern along the digestive system of the bugs, mostly occupying separate epithelial cells, unknown caeca-like organs, the Malpighian tubules and the salivary glands. This pattern differed between the two Macrolophus species: in M. pygmaeus, R. limoniae was distributed more broadly along the host digestive system and R. bellii was located primarily in the foregut and midgut. In contrast, in M. melanotoma, R. bellii was more broadly distributed along the digestive system than the clustered R. limoniae. Taken together, these results suggest that Rickettsia may have a role in the nutritional ecology of their plant-and prey-consuming hosts.

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Section: Discussionmentioning

confidence: 99%

Rickettsia association with two Macrolophus (Heteroptera: Miridae) species: A comparative study of phylogenies and within-host localization patterns

Dally

Izraeli²,

Belausov³

et al. 2023

Front. Microbiol.

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“…The WRA, WS, and S colonies were never found in the field; they “evolved” in the lab: the first two were originally WRSA, which have lost Sodalis (hence, WRA) or Rickettsia and Arsenophonus (hence WS); the S colony was generated during an experiment of horizontal transmission of WRS in which only Sodalis successfully established ( 53 ). This highlights that the symbiotic composition is dynamic and is dictated by selection pressures that differ between field and laboratory conditions ( 54 ).…”

Section: Discussionmentioning

confidence: 99%

“…The parasitoids were subsequently reared on house fly pupae under conditions of 26°C ± 1°C, 60% ± 20% RH, and 14 h photophase. The WRA, WS, and S colonies “evolved” in the lab: the first two were originally WRSA, which subsequently lost Sodalis (hence WRA) or Rickettsia and Arsenophonus (hence WS); the S colony was generated during an experiment of horizontal transmission of WRS into an uninfected line in which only Sodalis successfully established ( 53 ) (the number of founders of WRA, WS, and S was not recorded).…”

Section: Methodsmentioning

confidence: 99%

Abundance and Localization of Symbiotic Bacterial Communities in the Fly Parasitoid Spalangia cameroni

Shalom

Weiß

Lalzar

et al. 2022

Appl Environ Microbiol

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“…Such vectors can carry on the infection, either temporarily or permanently, and transmit it to a recipient species at a distant geographic location from the donor species. From preypredator (Johanowicz & Hoy, 1996;Le Clec'h et al, 2013) to host-parasitoid (Kageyama et al, 2010;Tzuri et al, 2020;Vavre et al, 1999) and trophallaxis interactions (Ramalho & Moreau, 2020), there are several direct and indirect ecological pathways which can be routes of Wolbachia transfer. Intimacy of direct physical interaction between ants and scale insects may provide a route of microbial exchange, as seen in other hemipteran groups (Ivens et al, 2018;Pringle & Moreau, 2017).…”

Section: Role Of Scale Insect Associate Species In Wolbachia Host Shiftsmentioning

confidence: 99%

Host phylogeny and ecological associations best explain Wolbachia host shifts in scale insects Running title: Wolbachia host shifts

Sanaei

Albery

Yeoh

et al. 2022

Preprint

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Wolbachia are among the most prevalent and widespread endosymbiotic bacteria on earth. Wolbachia’s success in infecting an enormous number of arthropod species is attributed to two features: the range of phenotypes they induce in their hosts, and their ability to switch between host species. Whilst much progress has been made in elucidating their induced phenotypes, our understanding of Wolbachia host shifting is still very limited: we lack answers to even fundamental questions concerning Wolbachia’s routes of transfer and the importance of factors influencing host shifts. Here, we investigate the diversity and host-shift patterns of Wolbachia in scale insects, a group of arthropods with intimate associations with other insects that make them well-suited to studying host shifts. Using Illumina pooled amplicon sequencing of Wolbachia-infected scale insects and their direct associates we determined the identity of all Wolbachia strains. We then fitted a Generalised Additive Mixed Model (GAMM) to our data to estimate the influence of host phylogeny and the geographic distribution on Wolbachia strain sharing among scale insect species. The model predicts no significant contribution of host geography but strong effects of host phylogeny, with high rates of Wolbachia sharing among closely related species and a sudden drop-off in sharing with increasing phylogenetic distance. We also detected the same Wolbachia strain in scale insects and several intimately associated species (ants, wasps, beetles, and flies). This indicates putative host shifts and potential routes of transfers via these associates and highlights the importance of ecological connectivity in Wolbachia host-shifting.

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Horizontal Transmission of Microbial Symbionts Within a Guild of Fly Parasitoids

Cited by 8 publications

References 51 publications

Rickettsia association with two Macrolophus (Heteroptera: Miridae) species: A comparative study of phylogenies and within-host localization patterns

Rickettsia association with two Macrolophus (Heteroptera: Miridae) species: A comparative study of phylogenies and within-host localization patterns

Abundance and Localization of Symbiotic Bacterial Communities in the Fly Parasitoid Spalangia cameroni

Host phylogeny and ecological associations best explain Wolbachia host shifts in scale insects Running title: Wolbachia host shifts

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