Horizontal transfer of large plasmid with type <scp>IV</scp> secretion system and mosquitocidal genomic island with excision and integration capabilities in <i>Lysinibacillus sphaericus</i>

Geng, Peiling; Cheng, Jiao; Yuan, Zhiming; Xiong, Hairong; Wang, Haiying; Hu, Xiaomin

doi:10.1111/1462-2920.15467

Cited by 6 publications

(2 citation statements)

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“…Conjugative and mobilizable elements transfer many kinds of traits, including virulence factors and genes involved in symbiosis ( Nuti et al 1979 ; Johnson and Nolan 2009 ; Carattoli 2013 ). Their ability to transfer large amounts of DNA per event allows the spread of complex traits encoded in long genetic loci ( Kobayashi 2018 ; Geng et al 2021 ). The host range of conjugation can be very broad, for example, conjugation can take place between Proteobacteria and Firmicutes ( Trieu-Cuot et al 1987 ) or from Proteobacteria to plants ( Lacroix and Citovsky 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Evolution of Plasmid Mobility: Origin and Fate of Conjugative and Nonconjugative Plasmids

Coluzzi

Garcillán-Barcia

Cruz

et al. 2022

Molecular Biology and Evolution

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Conjugation drives the horizontal transfer of adaptive traits across prokaryotes. One-fourth of the plasmids encode the functions necessary to conjugate autonomously, the others being eventually mobilizable by conjugation. To understand the evolution of plasmid mobility, we studied plasmid size, gene repertoires, and conjugation-related genes. Plasmid gene repertoires were found to vary rapidly in relation to the evolutionary rate of relaxases, e.g., most pairs of plasmids with 95% identical relaxases have fewer than 50% of homologs. Among 249 recent transitions of mobility type, we observed a clear excess of plasmids losing the capacity to conjugate. These transitions are associated with even greater changes in gene repertoires, possibly mediated by transposable elements, including pseudogenization of the conjugation locus, exchange of replicases reducing the problem of incompatibility, and extensive loss of other genes. At the micro-evolutionary scale of plasmid taxonomy, transitions of mobility type sometimes result in the creation of novel taxonomic units. Interestingly, most transitions from conjugative to mobilizable plasmids seem to be lost in the long term. This suggests a source-sink dynamic, where conjugative plasmids generate non-conjugative plasmids that tend to be poorly adapted and are frequently lost. Still, in some cases these relaxases seem to have evolved to become efficient at plasmid mobilization in trans, possibly by hijacking multiple conjugative systems. This resulted in specialized relaxases of mobilizable plasmids. In conclusion, the evolution of plasmid mobility is frequent, shapes the patterns of gene flow in bacteria, the dynamics of gene repertoires, and the ecology of plasmids.

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Section: Introductionmentioning

confidence: 99%

Evolution of Plasmid Mobility: Origin and Fate of Conjugative and Nonconjugative Plasmids

Coluzzi

Garcillán-Barcia

Cruz

et al. 2022

Molecular Biology and Evolution

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“…Conjugative and mobilizable elements transfer many kinds of traits, including virulence factors and symbiotic islands (Nuti et al 1979 ;Johnson, Nolan 2009 ;Carattoli 2013). Their ability to transfer large amounts of DNA per event allows the spread of complex traits encoded in long genetic loci (Kobayashi 2018;Geng et al 2021). The broad host range of conjugation allows transfer across bacterial Phyla and beyond, e.g.…”

Section: Introductionmentioning

confidence: 99%

Evolution of plasmid mobility: origin and fate of non-conjugative plasmids

Coluzzi

Garcillán-Barcia

Cruz

et al. 2021

Preprint

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Conjugation drives horizontal gene transfer of many adaptive traits across prokaryotes. Yet, only a fourth of the plasmids encode the functions necessary to conjugate autonomously, others being non-mobile or mobilizable by other elements. How these different plasmids evolve is poorly understood. Here, we studied plasmid evolution in terms of their gene repertoires and relaxases. We observed that gene content in plasmid varies rapidly in relation to the rate of evolution of relaxases, such that plasmids with 95% identical relaxases have on average fewer than 50% of homologs. The identification of 249 recent transitions in terms of mobility types revealed that they are associated with even greater changes in gene repertoires, possibly mediated by transposable elements that are more abundant in such plasmids. These changes include pseudogenization of the conjugation locus, exchange of replication initiators, and extensive gene loss. In some instances, the transition between mobility types also leads to the genesis of novel plasmid taxonomic units. Most of these transitions are short-lived, suggesting a source-sink dynamic, where conjugative plasmids constantly generate mobilizable and putatively non-mobilizable plasmids by gene deletion. Yet, in few cases such transitions resulted in the emergence of large clades of relaxases present only in mobilizable plasmids, suggesting successful specialization of these families in the hijacking of diverse conjugative systems. Our results shed further light on the huge plasticity of plasmids, suggest that many non-conjugative plasmids emerged recently from conjugative elements and allowed to quantify how changes in plasmid mobility shape the variation of their gene repertoires.

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