Horizontal Transfer of a Novel Soil Agarase Gene from Marine Bacteria to Soil Bacteria via Human Microbiota

Song, Tao; Xu, Hui; Wei, Congchong; Jiang, Tengfei; Qin, Siyu; Zhang, Weijia; Cao, Yu; Hu, Chao-Chin; Zhang, Fan; Qiao, Dan; Cao, Yiyi

doi:10.1038/srep34103

Cited by 15 publications

(10 citation statements)

References 47 publications

(63 reference statements)

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“…The authors hypothesised that the gut bacteria of native Okinawa participants may have acquired genes from marine bacteria that produce the digestive enzyme fucoidanase. This horizontal transfer of genes from ocean-dwelling bacteria that normally colonise and feed on seaweed has previously been reported in populations that have consumed seaweed for thousands of years [353][354][355][356][357].…”

Section: Bioaccessibility Of Seaweed Polysaccharidessupporting

confidence: 63%

Seaweed Components as Potential Modulators of the Gut Microbiota

2021

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Macroalgae, or seaweeds, are a rich source of components which may exert beneficial effects on the mammalian gut microbiota through the enhancement of bacterial diversity and abundance. An imbalance of gut bacteria has been linked to the development of disorders such as inflammatory bowel disease, immunodeficiency, hypertension, type-2-diabetes, obesity, and cancer. This review outlines current knowledge from in vitro and in vivo studies concerning the potential therapeutic application of seaweed-derived polysaccharides, polyphenols and peptides to modulate the gut microbiota through diet. Polysaccharides such as fucoidan, laminarin, alginate, ulvan and porphyran are unique to seaweeds. Several studies have shown their potential to act as prebiotics and to positively modulate the gut microbiota. Prebiotics enhance bacterial populations and often their production of short chain fatty acids, which are the energy source for gastrointestinal epithelial cells, provide protection against pathogens, influence immunomodulation, and induce apoptosis of colon cancer cells. The oral bioaccessibility and bioavailability of seaweed components is also discussed, including the advantages and limitations of static and dynamic in vitro gastrointestinal models versus ex vivo and in vivo methods. Seaweed bioactives show potential for use in prevention and, in some instances, treatment of human disease. However, it is also necessary to confirm these potential, therapeutic effects in large-scale clinical trials. Where possible, we have cited information concerning these trials.

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Section: Bioaccessibility Of Seaweed Polysaccharidessupporting

confidence: 63%

Seaweed Components as Potential Modulators of the Gut Microbiota

2021

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“…In addition, Song T. et al reported that aga1 (an agarase) may have been transferred together with its surrounding genes, from marine bacteria to soil bacteria via human microbiota. They speculated that microbes from inland humans may degrade agar and that these microbes may have acquired seaweed associated genes because of increased seaweed in diets [ 26 ]. Considering these reports, the inhabitants of Okinawa prefecture may have acquired digestive enzymes from mozuku in their gut bacteria because Okinawa mozuku is extensively consumed within this area [ 3 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Absorption Study of Mozuku Fucoidan in Japanese Volunteers

et al. 2018

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We performed an oral administration study of fucoidan in 396 Japanese volunteers and investigated significant factors concerning the absorption of fucoidan. Urine samples were collected at 0, 3, 6, and 9 h after ingestion of 3 g of fucoidan. Fucoidan was detected in urine after ingestion in 385 out of 396 subjects. The maximum value (mean ± standard deviation (SD)) of urinary fucoidan was 332.3 ± 357.6 μg/gCr in subjects living in Okinawa prefecture, compared with 240.1 ± 302.4 μg/gCr in subjects living outside Okinawa. Compared with the estimated urinary excretion of fucoidan by place of residence, those of subjects living in Okinawa prefecture were significantly higher than those living outside Okinawa prefecture (p < 0.01). In addition, subjects living in Okinawa prefecture consumed significantly greater amounts of mozuku compared with those living outside Okinawa prefecture (p < 0.01). Multiple regression analysis showed that having Okinawa prefecture as a place of residence was a significant factor (p < 0.01) contributing to the estimated urinary excretion of fucoidan. Because the habit of eating mozuku was significantly higher (p < 0.01) in subjects living in Okinawa prefecture than in those living outside Okinawa prefecture, the habit of eating mozuku was speculated to be a factor in the absorption of fucoidan.

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“…A novel agarase gene aga1 in an inland soil agar-degrading bacterium Paenibacillus sp. SSG-1 might be horizontally transferred from marine bacteria via human microbiota ( Song T. et al, 2016 ). In the genus Vibrio , horizontal gene transfer mainly contributes to the virulence of strains ( Le Roux and Blokesch, 2018 ; Deng et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomic and Secretomic Analysis Provide Insights Into Unique Agar Degradation Function of Marine Bacterium Vibrio fluvialis A8 Through Horizontal Gene Transfer

et al. 2020

Front. Microbiol.

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Agarose-oligosaccharide production from agar degradation by agarase exhibits lots of advantages and good application prospects. In this study, a novel agar-degrading bacterium Vibrio sp. A8 was isolated from a red algae in the South China Sea. The whole genome sequencing with comparative genomic and secretomic analysis were used to better understand its genetic components about agar degradation. This strain exhibited good agarase production in artificial seawater after culture optimization. The complete genome (4.88 Mb) of this strain comprised two circular chromosomes (3.19 and 1.69 Mb) containing 4,572 protein-coding genes, 108 tRNA genes and 31 rRNA genes. This strain was identified as Vibrio fluvialis A8 by comparative genomic analysis based on genome phylogenetic tree and average nucleotide identity (ANI) similarity. Different from other 20 similar strains including three strains of the same species, V. fluvialis A8 possessed unique agar degradation ability with four β-agarases (GH50) and one α-1,3-L-NA2 hydrolase (GH117) due to the horizontal gene transfer. Secretomic analysis showed that only β-agarase (gene 3152) was abundantly expressed in the secretome of V. fluvialis A8. This agarase had a good substrate specificity and wide work conditions in complex environments, suggesting its potential application for agarose-oligosaccharide production.

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Horizontal Transfer of a Novel Soil Agarase Gene from Marine Bacteria to Soil Bacteria via Human Microbiota

Cited by 15 publications

References 47 publications

Seaweed Components as Potential Modulators of the Gut Microbiota

Seaweed Components as Potential Modulators of the Gut Microbiota

Absorption Study of Mozuku Fucoidan in Japanese Volunteers

Comparative Genomic and Secretomic Analysis Provide Insights Into Unique Agar Degradation Function of Marine Bacterium Vibrio fluvialis A8 Through Horizontal Gene Transfer

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