Horizontal nDEP cages within open microwell arrays for precise positioning of cells and particles

Lombardini, Marta; Bocchi, Massimo; Rambelli, Laura; Giulianelli, Luca; Guerrieri, R.

doi:10.1039/b923567a

Cited by 7 publications

(4 citation statements)

References 23 publications

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“…A significant number of studies reported different types of DEP microfluidic devices for isolation and separation of particles based on the n‐DEP. The designs of such electrodes are parallel electrodes with an insulator between the electrodes, multipole electrodes (e.g., quadrupole, octupole), n‐DEP cages, cantilever type electrodes, planar electrodes with different shaped and geometry such as pearl‐shaped, circular, square in interdigitated type electrodes [20–28]. A lot of these electrodes were designed for concentration and immobilization of multiple particles simultaneously, rather than trapping and manipulation in single‐particle level.…”

Section: Introductionmentioning

confidence: 99%

“…A lot of these electrodes were designed for concentration and immobilization of multiple particles simultaneously, rather than trapping and manipulation in single‐particle level. For effective particle isolation using n‐DEP, an electrode capable to produce lower electric field gradient within a closed shape is more effective [26–30]. It is possible to generate a uniform lower electric gradient in a closed shape window in the electrode, which can serve as the trapping locations for the particles isolated inside the DEP device.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative analysis of the three‐dimensional trap stiffness of a dielectrophoretic corral trap

et al. 2021

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Dielectrophoresis is a robust approach for the manipulation and separation of (bio)particles using microfluidic platforms. We developed a dielectrophoretic corral trap in a microfluidic device that utilizes negative dielectrophoresis to capture single spherical polystyrene particles. Circular‐shaped micron‐size traps were employed inside the device and the three‐dimensional trap stiffness (restoring trapping force from equilibrium trapping location) was analyzed using 4.42 μm particles and 1 MHz of an alternating electric field from 6 VP‐P to 10 VP‐P. The trap stiffness increased exponentially in the x‐ and y‐direction, and linearly in the z‐direction. Image analysis of the trapped particle movements revealed that the trap stiffness is increased 608.4, 539.3, and 79.7% by increasing the voltage from 6 VP‐P to 10 VP‐P in the x‐, y‐, and z‐direction, respectively. The trap stiffness calculated from a finite element simulation of the device confirmed the experimental results. This analysis provides important insights to predict the trapping location, strength of the trapping, and optimum geometry for single particle trapping and its applications such as single‐molecule analysis and drug discovery.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of the three‐dimensional trap stiffness of a dielectrophoretic corral trap

et al. 2021

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“…The DEP force is non-contact and it involves low damage to cells. However, the DEP force is not sufficient to immobilize cells during changes in a medium with high flow velocity, as seen in [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Improved Laser Manipulation for On-chip Fabricated Microstructures Based on Solution Replacement and Its Application in Single Cell Analysis

Yue

Nakajima

Takeuchi

et al. 2014

International Journal of Advanced Robotic Systems

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In this paper, we present the fabrication and assembly of microstructures inside a microfluidic device based on a photocrosslinkable resin and optical tweezers. We also report a method of solution replacement inside the microfluidic channel in order to improve the manipulation performance and apply the assembled microstructures for single cell cultivation. By the illumination of patterned ultraviolet (UV) through a microscope, microstructures of arbitrary shape were fabricated by the photocrosslinkable resin inside a microfluidic channel. Based on the microfluidic channel with both glass and polydimethylsiloxane (PDMS) surfaces, immovable and movable microstructures were fabricated and manipulated. The microstructures were fabricated at the desired places and manipulated by the optical tweezers. A rotational microstructure including a microgear and a rotation axis was assembled and rotated in demonstrating this technique. The improved laser manipulation of microstructures was achieved based on the on-chip solution replacement method. The manipulation speed of the microstructures increased when the viscosity of the solvent decreased. The movement efficiency of the fabricated microstructures inside the lower viscosity solvent was evaluated and compared with those microstructures inside the former high viscosity solvent. A novel cell cage was fabricated and the cultivation of a single yeast cell (w303) was demonstrated in the cell cage, inside the microfluidic device.

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“…[8,9] This kind of physical problem could be found in many microfluidic applications such as polydimethylsiloxane (PDMS) microchannels with open outlets. [11,12] The novel performance of the Marangoni flow has potential applications in micropumping with a totally different mechanism from previous ones. [13,14] The T-junction is formed by a main channel and a side channel (Fig.…”

mentioning

confidence: 99%

Marangoni Bifurcation Flow in a Microchannel T-Junction and Its Micropumping Effect: A Computational Study

Zhang¹,

Wang²,

Zhou³

2012

Chinese Phys. Lett.

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We discuss a bifurcation phenomenon of Marangoni flow in a microchannel T-junction and its novel unidirectional pumping effect. The T-junction is formed by a main channel connected with a liquid reservoir and a side channel outlet to the atmosphere. A volatizing meniscus is formed in the side channel and Marangoni convections are generated due to the non-uniform evaporation on the meniscus. It is found for weak evaporations (𝑀 𝑎 < 270) the Marangoni convections are symmetrical. However, for intense evaporations (𝑀 𝑎 > 270), the initial inward symmetrical Marangoni convection becomes unstable and converted into one single vortex flow. Moreover, the single vortex induces a steady unidirectional flow in the main channel, acting like a pump.

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Horizontal nDEP cages within open microwell arrays for precise positioning of cells and particles

Cited by 7 publications

References 23 publications

Quantitative analysis of the three‐dimensional trap stiffness of a dielectrophoretic corral trap

Quantitative analysis of the three‐dimensional trap stiffness of a dielectrophoretic corral trap

Improved Laser Manipulation for On-chip Fabricated Microstructures Based on Solution Replacement and Its Application in Single Cell Analysis

Marangoni Bifurcation Flow in a Microchannel T-Junction and Its Micropumping Effect: A Computational Study

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