Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in<i>Vibrio cholerae</i>

Morita, Masatomo; Yamamoto, Shouji; Hiyoshi, Hirotaka; Kodama, Takao; Okura, Masatoshi; Arakawa, Eiji; Alam, Munirul; Ohnishi, Makoto; Izumiya, Hidemasa; Watanabe, Haruo

doi:10.1111/j.1348-0421.12039

Cited by 2 publications

(2 citation statements)

References 4 publications

(5 reference statements)

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“…The number of trans ferable genes induced by competence varies considerably in different microorganisms. It is supposed that in natural ecosystems there is horizontal gene transfer of a type III secretion system among V. cholerae strains [44]. The fact of replacement of the whole gene cluster in O1 specific antigen of V. cholerae eltor to the gene clusters of O37 or O139 antigens by means of natural competence induced by chitin was noted [45].…”

Section: Role Of Chitin In Natural Competence Of V Choleraementioning

confidence: 99%

Chitin and products of its hydrolysis in Vibrio cholerae ecology

Markov

Куликалова

Urbanovich

et al. 2015

Biochemistry Moscow

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The role of chitin and its hydrolysis products generated by Vibrio cholerae chitinases in mechanisms of its adaptation in water environments, metabolism, preservation, acquisition of pathogenic potential, and its epidemiological value are reviewed. Chitin utilization by V. cholerae as a source of energy, carbon, and nitrogen is described. Chitin association promotes biofilm formation on natural chitinous surfaces, increasing V. cholerae resistance to adverse factors in ecological niches: the human body and water environments with its inhabitants. Hydrolytic enzymes regulated by the corresponding genes result in complete chitin biodegradation by a chitinolytic catabolic cascade. Consequences of V. cholerae cell and chitin interaction at different hierarchical levels include metabolic and physiological cell reactions such as chemotaxis, cell division, biofilm formation, induction of genetic competence, and commensalic and symbiotic mutual relations with higher organisms, nutrient cycle, pathogenicity for humans, and water organisms that is an example of successful interrelation of bacteria and substratum in the ecology of the microorganism.

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Section: Role Of Chitin In Natural Competence Of V Choleraementioning

confidence: 99%

Chitin and products of its hydrolysis in Vibrio cholerae ecology

Markov

Куликалова

Urbanovich

et al. 2015

Biochemistry Moscow

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“…In contrast, most clinically isolated non‐O1/non‐O139 strains do not encode TCP or CT, utilizing less well understood mechanisms to colonize host tissues and alter intestinal cell homeostasis. We now know that a subset of non‐O1/non‐O139 serogroup strains isolated from cholera patients carry genes for a Type 3 Secretion System (T3SS), and studies have shown that the T3SS is required for disease in murine and rabbit animal models of infection (Dziejman et al ., ; Tam et al ., ; Rahman et al ., ; Chatterjee et al ., ; Tam et al ., ; Shin et al ., ; Dutta et al ., ; Hasan et al ., ; Luo et al ., ; Morita et al ., ; Octavia et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Characterization ofV.choleraeT3SS-dependent cytotoxicity in cultured intestinal epithelial cells

Miller

Chaand

Gregoire

et al. 2016

Cellular Microbiology

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Summary AM-19226 is a pathogenic, non-O1/non-O139 serogroup strain of Vibrio cholerae that uses a Type 3 Secretion System (T3SS) mediated mechanism to colonize host tissues and disrupt homeostasis, causing cholera. Co-culturing the Caco2-BBE human intestinal epithelial cell line with AM-19226 in the presence of bile results in rapid mammalian cell death that requires a functional T3SS. We examined the role of bile, sought to identify the mechanism, and evaluated the contributions of T3SS translocated effectors in in vitro cell death. Our results suggest that Caco2-BBE cytotoxicity does not proceed by apoptotic or necrotic mechanisms, but rather displays characteristics consistent with osmotic lysis. Cell death was preceded by disassembly of epithelial junctions and reorganization of the cortical membrane skeleton, although neither cell death nor cell-cell disruption required VopM or VopF, two effectors known to alter actin dynamics. Using deletion strains, we identified a subset of AM-19226 Vops that are required for host cell death, which were previously assigned roles in protein translocation and colonization, suggesting that they function other than to promote cytotoxicity. The collective results therefore suggest that cooperative Vop activities are required to achieve cytotoxicity in vitro, or alternatively, that translocon pores destabilize the membrane in a bile dependent manner.

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Horizontal gene transfer of a genetic island encoding a type III secretion system distributed inVibrio cholerae

Cited by 2 publications

References 4 publications

Chitin and products of its hydrolysis in Vibrio cholerae ecology

Chitin and products of its hydrolysis in Vibrio cholerae ecology

Characterization ofV.choleraeT3SS-dependent cytotoxicity in cultured intestinal epithelial cells

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