Horizontal Gene Transfer Is the Main Driver of Antimicrobial Resistance in Broiler Chicks Infected with Salmonella enterica Serovar Heidelberg

Oladeinde, Adelumola; Abdo, Zaid; Press, Maximilian O.; Cook, Kimberly J.; Cox, N. A.; Zwirzitz, Benjamin; Woyda, Reed; Lakin, Steven M.; Thomas, Jesse C.; Looft, Torey; Cosby, D. E.; Hinton, Arthur; Guard, Jean; Line, Eric; Rothrock, Michael J.; Berrang, M. E.; Herrington, Kyler; Zock, Gregory; Lawrence, Jodie Plumblee; Cudnik, Denice; House, Sandra L.; Ingram, K. D.; Lariscy, Leah; Wagner, Martin; Aggrey, Samuel E.; Chai, Lilong; Ritz, Casey W.

doi:10.1128/msystems.00729-21

Cited by 9 publications

(22 citation statements)

References 88 publications

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“…The MDR phenotype is widespread in various Salmonella serovars including those infecting farm animals and humans, thus presenting a challenge in current first-line therapies [13][14][15]. Clonal dissemination of MDR clones is a significant concern [16,17]. However, initial clonal strains might diversify by incorporating various genetic elements that can be incorporated into the bacterial chromosome or remain episomal, such as the pESI plasmid [18].…”

Section: Introductionmentioning

confidence: 99%

Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile

et al. 2021

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Salmonella comprises over 2500 serotypes and foodborne contamination associated with this pathogen remains an important health concern worldwide. During the last decade, a shift in serotype prevalence has occurred as traditionally less prevalent serotypes are increasing in frequency of infections, especially those related to poultry meat contamination. S. Infantis is one of the major emerging serotypes, and these strains commonly display antimicrobial resistance and can persist despite cleaning protocols. Thus, this work aimed to isolate S. Infantis strains from a poultry meat farm in Santiago, Chile and to characterize genetic variations present in them. We determined their genomic and phenotypic profiles at different points along the production line. The results indicate that the strains encompass 853 polymorphic sites (core-SNPs) with isolates differing from one another by 0–347 core SNPs, suggesting variation among them; however, we found discrete correlations with the source of the sample in the production line. Furthermore, the pan-genome was composed of 4854 total gene clusters of which 2618 (53.9%) corresponds to the core-genome and only 181 (3.7%) are unique genes (those present in one particular strain). This preliminary analysis will enrich the surveillance of Salmonella, yet further studies are required to assess their evolution and phylogeny.

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Section: Introductionmentioning

confidence: 99%

Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile

et al. 2021

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“…Heidelberg and raised the chicks on either fresh pine shavings (here after referred to as fresh litter) (n = 75) or reused litter (litter previously used to raise three flocks of broiler chickens) (n =75). Chicks were challenged either through oral gavage (n = 25), cloacal inoculation (n =25), or by the seeder method (i.e., a few chicks [n = 5] were challenged orally and comingled with unchallenged chicks [n = 20]) [7]. Unchallenged chicks on fresh (n =25) and reused litter (n =25) were used as controls.…”

Section: Resultsmentioning

confidence: 99%

“…2). Thirty-one percent of isolates from the ceca (37/118) and 37.5 % from the litter (15/40) of chicks on fresh litter acquired resistance to gentamicin, tetracycline, and streptomycin [7] (Fig. 2a).…”

Section: Resultsmentioning

confidence: 99%

“…The percentage of S . Heidelberg isolates that acquired AMR in fresh litter differed by the route of inoculation [7]. For instance, 40% of isolates from orally challenged chicks acquired AMR compared to 24% for cloacal [7].…”

Section: Resultsmentioning

confidence: 99%

“…Heidelberg strain and raised antibiotic-free on fresh litter composed of pine shavings were colonized at a high rate with S . Heidelberg strains that harbored IncI1 plasmids encoding AMR [7]. We selected S .…”

Section: Introductionmentioning

confidence: 99%

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Litter commensal bacteria can limit the horizontal gene transfer of antimicrobial resistance to Salmonella in chickens

Oladeinde

Abdo

Zwirzitz

et al. 2021

Preprint

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Host microbiome homeostasis ensures that gut conditions are unfavorable to an invading pathogen such as Salmonella enterica. Consequently, fostering a 'balanced' gut microbiome through the administration of microbes that can competitively exclude pathogens has gained a lot of attention and use in human and animal medicine. However, little is known on how competitive exclusion affects the transfer of antibiotic resistance. To shed more light on this question, we challenged neonatal broiler chicks raised on reused broiler chicken litter – a complex environment comprising of decomposing pine shavings, feces, uric acid, feathers, and feed, with Salmonella Heidelberg (S. Heidelberg), a model pathogen. We show that chicks raised on reused litter carried lower abundance of Salmonella and harbored a more uniform and diverse microbiome comprising of bacterial species that are known to provide colonization resistance towards Salmonella compared to chicks raised on fresh bedding composed of pine shavings. Additionally, these bacterial species were associated with a lower horizontal transfer of multidrug resistance genes to S. Heidelberg. Using in vitro competition experiments, we confirmed that conjugation between S. Heidelberg and E. coli strains from chicks raised on fresh litter resulted in the acquisition of multidrug resistant plasmids. Contrastingly, bacteriophage-mediated recombination between S. Heidelberg and E. coli strains made the acquisition of plasmid-mediated β-lactamase gene (blaCMY-2)possible. Collectively, this study demonstrates that competitive exclusion can reduce the transfer of antibiotic resistance and provides information on the bacterial species that can be explored for their benefits to reduce antibiotic resistance transfer.

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Occurrence characteristics and influencing factors of antibiotic resistance genes in rural groundwater in Henan Province

Zhang,

Li,

et al. 2024

Environ Sci Pollut Res

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Horizontal Gene Transfer Is the Main Driver of Antimicrobial Resistance in Broiler Chicks Infected with Salmonella enterica Serovar Heidelberg

Cited by 9 publications

References 88 publications

Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile

Genetic Characterization of Salmonella Infantis with Multiple Drug Resistance Profiles Isolated from a Poultry-Farm in Chile

Litter commensal bacteria can limit the horizontal gene transfer of antimicrobial resistance to Salmonella in chickens

Occurrence characteristics and influencing factors of antibiotic resistance genes in rural groundwater in Henan Province

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