Horizontal Chromatography Accelerating Apparatus. Description of Apparatus and Applications.

Abstract

An apparatus is described which permits horizontal chromatography, or ion exchange separations, to be carried out on strips of media, or circular disks. The apparatus permits these techniques to be accelerated at choice by the introduction of rapid solvent flow rate, heat, and centrifugal force. This apparatus has found use in accelerating a wide variety of chromatographic separations.

Horizontal Chromatography Accelerating Apparatus. Separation of Dyes and Indicators.

“…The operation of the centrifugally accelerated horizontal chromatography apparatus has been described in detail (2). The apparatus was operated with an automatic solvent delivery systen/in the present studies.…”

Application and Theory of Unidimensional Multiple Chromatography.

“…The superb resolving power of UMC is achieved by effectively increasing the length of the chromatographic support and the number of theoretical plates over which the solutes migrate. Although UMC is capable of excellent resolution of simple mixtures (10,19), the excessive time required for multiple irrigations has probably curtailed its use. In recent years, however, centrifugally accelerated chromatography (5,18,26,27) and thin-layer chromatography, TLC [for recent reviews, see (33,44) ], have been introduced as analytical tools and both of these innovations have the advantage of decreasing solvent development time. For this reason, it is now feasible to employ a larger number of solvent passes when attempting to resolve simple mixtures.…”

“…Taking the logarithm of Equation 17gives: pln,(l -Rn) = gln. (l -Rn + 1) (18) Then substituting for R" and R" + i in terms of Martin's modified equation (10)…”