Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil

Villegas-Torres, María F.; Bedoya-Reina, Oscar C.; Salazar, Camilo; Vives, Martha J.; Dussán, Jenny

doi:10.1016/j.ibiod.2010.10.007

Cited by 56 publications

(31 citation statements)

References 22 publications

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“…Phylogenetic analysis of arsenate reductases from these species revealed a common ancestral origin of arsenate reductase and their subsequent horizontal transfer (Jackson and Dugas 2003). The events of horizontal gene transfer occur as a mechanism of environmental adaptability (Nemergut et al 2004) to maintain the fitness among bacteria inhabiting polluted habitats (Martinez et al 2006) and can be favoured by the occurrence of high concentration of heavy metals like arsenic (Cai et al 2009;Cavalca et al 2010;Villegas-Torres et al 2011). Hence, it appears that the arsenic-resistant bacteria inhabiting the Mandovi and Zuari estuarine network might have acquired the arsenic resistance genes from other bacteria by means of horizontal gene transfer.…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of arsenic-resistant estuarine bacteria and characterization of their ars genotype

et al. 2011

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In the present study, 44 arsenic-resistant bacteria were isolated through serial dilutions on agar plate with concentrations ≥0.05 mM of sodium arsenite and ≥10 mM of sodium arsenate from Mandovi and Zuari--estuarine water systems. The ars genotype characterization in 36 bacterial isolates (resistant to 100 mM of sodium arsenate) revealed that only 17 isolates harboured the arsA (ATPase), B (arsenite permease) and C (arsenate reductase) genes on the plasmid DNA. The arsA, B and C genes were individually detected using PCR in 16, 9 and 13 bacterial isolates respectively. Molecular identification of the 17 isolates bearing the ars genotype was carried using 16S rDNA sequencing. A 1300 bp full length arsB gene encoding arsenite efflux pump and a 409 bp fragment of arsC gene coding for arsenate reductase were isolated from the genera Halomonas and Acinetobacter. Phylogenetic analysis of arsB and arsC genes indicated their close genetic relationship with plasmid borne ars genes of E. coli and arsenate reductase of plant origin. The putative arsenate reductase gene isolated from Acinetobacter species complemented arsenate resistance in E. coli WC3110 and JM109 validating its function. This study dealing with isolation of native arsenic-resistant bacteria and characterization of their ars genes might be useful to develop efficient arsenic detoxification strategies for arsenic contaminated aquifers.

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Section: Discussionmentioning

confidence: 99%

Molecular identification of arsenic-resistant estuarine bacteria and characterization of their ars genotype

et al. 2011

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“…[30] Minimal salt broth [31] supplemented with different concentrations (600 µg/mL, 800 µg/mL, 1000 µg/mL and 1200 µg/mL) of Cr(VI) was used to determine the MIC for chromium. One percent inoculum of B2-DHA was added to the media in 50 mL falcon tubes.…”

Section: Minimum Inhibitory Concentration (Mic) Of Chromate and Othermentioning

confidence: 99%

Bioremediation of hexavalent chromium (VI) by a soil-borne bacterium,Enterobacter cloacaeB2-DHA

Rahman

Nahar

Nawani³

et al. 2015

Journal of Environmental Science and Health, Part A

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Bioremediation of hexavalent chromium (VI) by a soil borne bacterium, Enterobacter cloacae B2-DHA. Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances andEnvironmental Engineering, 50 (11) AbstractChromium and chromium containing compounds are discharged into the nature as waste from anthropogenic activities, such as industries, agriculture, forest farming, mining and metallurgy. Continued disposal of these compounds to the environment leads to development of various lethal diseases in both humans and animals. In this paper, we report a soil borne bacterium, B2-DHA that can be used as a vehicle to effectively remove chromium from the contaminated sources. B2-DHA is resistant to chromium with a MIC value of 1000 µg/mL has the potential to reduce chromium significantly to safe levels from the contaminated environments and suggest the potential use of this bacterium in reducing human exposure to chromium, hence avoiding poisoning.

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“…Phytoremediation can be showed in many ways. Phytoaccumualtion/Phytoextraction is the elimination of metals from polluted soils whereby the metal is extracted from the soil, and then translocated to, and concentrated in, the harvestable parts of the plants [83][84][85]. Many of these species of plants are proficient of hoarding non-essential heavy metals, including As and Pb, into the plant roots, but fewer can mass the metals into the aerial/harvestable parts for the plant.…”

Section: Phytoremediationmentioning

confidence: 99%

Microbes as Potential Tool for Remediation of Heavy Metals: A Review

Gupta¹,

Joia²,

Sood³

et al. 2016

J Microb Biochem Technol

198

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Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil

Cited by 56 publications

References 22 publications

Molecular identification of arsenic-resistant estuarine bacteria and characterization of their ars genotype

Molecular identification of arsenic-resistant estuarine bacteria and characterization of their ars genotype

Bioremediation of hexavalent chromium (VI) by a soil-borne bacterium,Enterobacter cloacaeB2-DHA

Microbes as Potential Tool for Remediation of Heavy Metals: A Review

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