Hop-derived Xanthohumol Induces HL-60 Leukemia Cells Death

Abstract

Background: Acute promyelocytic leukemia (APL) affects both kids and adults, however it is more prevalent in younger population. Although APL has a favorable prognostic, patients that relapse often do not respond positively to additional chemotherapy. Therefore, there is a need to further identify ways to overcome these challenges. Hypothesis: In this study, we examined antileukemic effects of xanthohumol (XN), a prenylated flavonoid derived from hops (Humulus lupulus L), on human promyelocytic HL-60 cells. Materials and Methods: HL-60 cells were exposed to different concentrations of XN (μM) for 24 h. Cell viability, cell morphology, chromatin condensation, cPARP-1 level, and caspase-3 activation, and the expression of p21 WAF1/Cip1 were analyzed. Results: XN reduced HL-60 cell viability in a dose-dependent manner. XN induced a dosedependent morphological changes including cell shrinkage and blebbing, and significantly increased IJBCRR, 29(1): 61-72, 2020; Article no.IJBCRR.53095 62 the number of cells with condensed chromatin. XN significantly increased the level of cPARP-1, active caspase-3, and the expression of p21WAF/CIP mRNA. Conclusion: These data indicate that XN induces HL-60 cell death by regulating cell cycle progression and apoptosis. This study suggests that XN may have antileukemic preventive effects. Original Research Article

Molecular Action of Polyphenols in Leukaemia and Their Therapeutic Potential

“… Chemical structures of all polyphenols discussed within this review. Aloe-emodin [ 29 ], Apigenin [ 30 ], Bestatin [ 31 ], Butein [ 32 ], Carnosic acid [ 33 ], Catechin [ 34 ], Chrysin [ 35 ], Curcumin [ 36 ], Delphinidin [ 37 ], Epigallocatechin gallate [ 38 ], Ellagic acid [ 39 ], Emodin [ 40 ], Epicatechin [ 41 ], Epsilon-viniferin [ 42 ], Flavopiridol [ 43 ], Gallic acid [ 44 ], Genistein [ 45 ], Guggulsterone [ 46 ], Icariside II [ 47 ], Luteolin [ 48 ], Piceatannol [ 49 ], Pinosylvin [ 50 ], Punicalagin [ 51 ], Quercetin [ 50 ], Resveratrol [ 52 ], Retinoic acid [ 53 ], Rhein [ 54 ], Silibinin [ 55 ], Cis -Stilbene [ 56 ], Trans -Stilbene [ 50 ], Tannic acid [ 57 ], Vitisin B [ 58 ], Xanthohumol [ 59 ]. …”