Honeybush (<i>Cyclopia</i> spp.) anther-stigma distance and intraspecies cross compatibilities

Koen, J.; Slabbert, M.M.; Booyse, M.; Bester, C.

doi:10.1080/02571862.2020.1815879

Cited by 3 publications

(3 citation statements)

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“…Mass flowering of cultivated populations forms a powerful attractant to pollinators, increasing local pollinator density ( Holzschuh et al, 2011 ; Westphal, Steffan-Dewenter & Tscharntke, 2003 ). This facilitates the spread of genetic material from cultivated populations into the wild via pollen flow, particularly in outcrossing species ( Ellstrand, Prentice & Hancock, 1999 ), such as members of Cyclopia ( Koen et al., 2021 ). By altering local allele frequencies through the introduction of large genetically depauperate commercial plantations, a landscape wide genetic bottleneck may result, promoting erosion of wild genetic diversity.…”

Section: Discussionmentioning

confidence: 99%

The transition to agricultural cultivation of neo-crops may fail to account for wild genetic diversity patterns: insights from the Cape Floristic Region

Galuszynski

2021

PeerJ

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Aim The global increase in the cultivation of native wild plants has raised concerns regarding potential risks associated with translocating genetic lineages beyond their natural range. This study aimed to investigate whether agricultural cultivation of neo-crops (a) accounts for the levels of genetic diversity present in wild populations, and whether (b) cultivated populations are genetically divergent from wild populations and thus pose a potential threat to wild genetic diversity. Location The Cape Floristic Region (CFR), located along the southern Cape of South Africa. Methods High Resolution Melt analysis (HRM) coupled with Sanger sequencing was used to screen three non-coding chloroplast DNA loci in Cyclopia Vent. (Fabaceae), a CFR endemic neo-crop cultivated for the production of a herbal infusion referred to as Honeybush tea. Wild and cultivated populations for three of three widely cultivated Honeybush species (C. intermedia, C. longifolia, and C. subternata) were screened. Genetic diversity and differentiation were measured and compared between wild and cultivated groups. Results Across all asseccions, a total of 17 haplotypes were detected, four of which were shared between wild and cultivated populations, while the remaining 13 were only detected in wild populations. Genetic diversity and differentiation was significantly higher in wild populations than in cultivated populations. Conclusions If no guidelines exist to facilitate the introduction of native wild plant taxa to a cultivated setting, wild genetic diversity patterns are likely to be compromised by cultivated populations. In the case presented here, cultivation represents a genetic bottleneck, failing to account for rare haplotypes, and may have disrupted species boundaries by initiating interspecific hybridization. More empirical work is required to evaluate the extent to which neo-crop cultivation poses a risk to wild genetic resources in the CFR and globally.

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Section: Discussionmentioning

confidence: 99%

The transition to agricultural cultivation of neo-crops may fail to account for wild genetic diversity patterns: insights from the Cape Floristic Region

Galuszynski

2021

PeerJ

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“…Volgens Penfield en MacGregor (2016), kan selfs geringe verskille in moederplant en/of omgewingsfaktore tot saaddimorfisme aanleiding gee, omdat die saadhuid 'n "hoogs plastiese orgaan" is, m.a.w baie reaktief op omgewingsseine (ongeag of dit deur die moedergenotipe of 'n direkte reaksie op die sigoot is). Onlangse studies op heuningbosspesies het sommige van hierdie raaisels begin ontrafel betreffende die blomstruktuur en stuifmeelkiemkragtigheid, wat ook 'n effek op die interaksies tussen bestuiwing en gevolglik suksesvolle bestuiwing kan hê (Koen et al, 2020a;Koen et al, 2020b)…”

Section: Effek Van Saadposisieunclassified

Honeybush ( Cyclopia spp.) mature embryo culture and the influence of pod position on seed colour

Koen¹,

Slabbert

Booyse³

et al. 2021

Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie

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Plante (verskillende genotipes) van Cyclopia spesies word gebruik om Suid-Afrikaanse heuningbostee (’n kruietee produk) te produseer en word tans deur teling vir grootskaalse verbouing verbeter. Saaddimorfisme en dormansie beïnvloed egter die ontkiemingspersentasie negatief. Deur gebruik te maak van volwasse embrioweefselkulture is studies om die ontkiemingspersentasie van saad te verhoog op C. longifolia, C. maculata en C. subternata gedoen. Die effek van stratifikasie op saadontkieming deur geen of ’n kouebehandeling toe te pas, is ook ondersoek. Volwasse embrios is hierna uit sade verwyder en vir ’n 24-uur fotoperiode by 25°C ± 1°C op weefselkultuur gekweek. Die kweek van volwasse embrios deur middel van weefselkulture was suksesvol en het deurlopend meer plante vergeleke met konvensionele saaimetode gelewer. Studies om die effek wat saadpeulposisie van die moederplant op saadkleur het, is ook gedoen. Volwasse heuningbospeule is vanaf die boonste, middelste en onderste-derdes van die blaarbedekte deel van die heuningbosplante versamel om die aantal groen/intermediêre/bruin sade wat by elke gedeelte geproduseer is, te bepaal. Die verhouding van saaddimorfisme wat by elke gedeelte geproduseer word, kon egter nie deur peulposisie voorspel word nie.

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“…Cyclopia species are among the few wild plants that have been turned into commercial commodities in South Africa. Although 23 species grow in the wild, only a few are currently used to make tea, including C. subternata, C. intermedia, C. genistoides, C. maculata, C. longifolia, and C. sessiliflora [3,4]. The popularity of honeybush can also be attributed to its low tannin Plants 2023, 12, 2181 2 of 18 content, the absence of caffeine, and the presence of antioxidants.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Differentially Expressed Proteins and Metabolic Pathways of Honeybush (Cyclopia subternata) in Response to Water Deficit Stress

Mahlare,

Husselmann,

Lewu

et al. 2023

Plants

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Honeybush (Cyclopia spp.) is a rich source of antioxidant properties and phenolic compounds. Water availability plays a crucial role in plant metabolic processes, and it contributes to overall quality. Thus, this study aimed to investigate changes in molecular functions, cellular components, and biological processes of Cyclopia subternata exposed to different water stress conditions, which include well-watered (as Control, T1), semi-water stressed (T2), and water-deprived (T3) potted plants. Samples were also collected from a well-watered commercial farm first cultivated in 2013 (T13) and then cultivated in 2017 (T17) and 2019 (T19). Differentially expressed proteins extracted from C. subternata leaves were identified using LC-MS/MS spectrometry. A total of 11 differentially expressed proteins (DEPs) were identified using Fisher’s exact test (p < 0.00100). Only α-glucan phosphorylase was found to be statistically common between T17 and T19 (p < 0.00100). Notably, α-glucan phosphorylase was upregulated in the older vegetation (T17) and downregulated in T19 by 1.41-fold. This result suggests that α-glucan phosphorylase was needed in T17 to support the metabolic pathway. In T19, five DEPs were upregulated, while the other six were downregulated. Based on gene ontology, the DEPs in the stressed plant were associated with cellular and metabolic processes, response to stimulus, binding, catalytic activity, and cellular anatomical entity. Differentially expressed proteins were clustered based on the Kyoto Encyclopedia of Genes and Genomes (KEGG), and sequences were linked to metabolic pathways via enzyme code and KEGG ortholog. Most proteins were involved in photosynthesis, phenylpropanoid biosynthesis, thiamine, and purine metabolism. This study revealed the presence of trans-cinnamate 4-monooxygenase, an intermediate for the biosynthesis of a large number of substances, such as phenylpropanoids and flavonoids.

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Honeybush (Cyclopia spp.) anther-stigma distance and intraspecies cross compatibilities

Cited by 3 publications

References 24 publications

The transition to agricultural cultivation of neo-crops may fail to account for wild genetic diversity patterns: insights from the Cape Floristic Region

The transition to agricultural cultivation of neo-crops may fail to account for wild genetic diversity patterns: insights from the Cape Floristic Region

Honeybush ( Cyclopia spp.) mature embryo culture and the influence of pod position on seed colour

Analysis of the Differentially Expressed Proteins and Metabolic Pathways of Honeybush (Cyclopia subternata) in Response to Water Deficit Stress

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