Honeybee Gut: Reservoir of Probiotic Bacteria

Tootiaie, Samira; Moharrami, Mojtabah; Mojgani, Naheed

doi:10.1007/978-981-16-0223-8_9

Cited by 3 publications

(3 citation statements)

References 59 publications

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“…2021; Toutiaee et al . 2021). Although, evidences exist showing the effectiveness of the probiotic bacteria in the same host from which it was originally isolated (Ptaszyńska et al .…”

Section: Resultsmentioning

confidence: 99%

In vitro probiotic and safety attributes of Bacillus spp. isolated from beebread, honey samples and digestive tract of honeybees Apis mellifera

Toutiaee

Mojgani

Harzandi

et al. 2022

Letters in Applied Microbiology

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Bacillus species isolated from honeybee Apis mellifera gut, honey and bee bread samples were characterized for their in vitro probiotic and safety attributes. Alpha and γ haemolytic cultures were tested for their antibiotic resistance, antibacterial spectrum, acid and bile tolerance, adhesion ability (auto‐aggregation, co‐aggregation and hydrophobicity) and phenol tolerance. Safety criteria included evaluation of virulence genes and cytotoxicity percentages. Bacillus isolates inhibited both Gram‐positive and Gram‐negative pathogens including Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus mutans, while none could inhibit Listeria monocytogenes. Among the isolates, Bacillus subtilis ZH05, ZB03 and ZG025 showed resistance to most of the tested antibiotics and were considered unsafe. B. subtilis (4) and B. licheniformis (1) tolerated acidic pH and bile conditions, never the less were more tolerant in simulated intestinal conditions vis‐a‐vis gastric conditions. In 0·5% phenol concentrations, B. licheniformis ZH02 showed highest growth, while, B. subtilis ZG029 demonstrated highest auto‐aggregation (65 ± 4·6) and hydrophobicity (23 ± 3·6) percentages (P < 0·05). The isolates lacked virulence genes (hblA, hblC, hblD, nhe, cytK and ces), and their cytotoxic percentage on Caco‐2 cell lines was ˂15%. Overall, honeybees appear to be a good source of Bacillus species exhibiting typical in vitro probiotic properties, which could be of commercial interest.

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“…2021; Toutiaee et al . 2021). Although, evidences exist showing the effectiveness of the probiotic bacteria in the same host from which it was originally isolated (Ptaszyńska et al .…”

Section: Resultsmentioning

confidence: 99%

In vitro probiotic and safety attributes of Bacillus spp. isolated from beebread, honey samples and digestive tract of honeybees Apis mellifera

Toutiaee

Mojgani

Harzandi

et al. 2022

Letters in Applied Microbiology

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“…Lactic Acid Bacteria, Bifidobacteria and Bacillus species, are the major groups which could mitigate the antibiotic-associated microbiota dysbiosis and immune deficits in adult workers (Daisley et al, 2020). The benefits of Bacillus species lie in their ability to produce a number of enzymes and antibiotic-like substances that could assist in the digestion of carbohydrates and suppress the growth of pathogenic or nocuous organisms in honey bees, respectively (Teo et al, 2005;Tootiaie et al, 2021). By assessing their health benefits, a number of these bacteria are considered probiotics and could exert desirable health effects on the host when consumed in adequate quantities (Khalkhali et al, 2017;Mojgani et al, 2015).…”

Section: Microbial Flora Of the Digestive Tractmentioning

confidence: 99%

Comparative effect of probiotic and antibiotic on honey bees colony functional traits

Besharati,

Bavand,

Paya

et al. 2024

The EuroBiotech Journal

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The purpose of this study was to evaluate the effect of different levels of probiotic on colony functional traits, honey quantitative and qualitative parameters and gut microbial flora in honey bees. A total of 56 hives were randomly allocated to 8 treatments with 7 replicates in the city of Firouzkoh (Iran) for about 60 days. Experimental treatments that were included: control, 2 g antibiotic, 1 g probiotic, 2 g probiotic, 3 g probiotic, 1 g antibiotic + 1 g probiotic, all per liter of syrup for each hive. The results showed that hives fed with treatments 2 g or 3 g probiotic per liter of syrup had higher hive population and honey production than the other treatments (p<0.05). Adding 2 g or 3 g probiotic per liter of syrup increased lactobacillus counts in bees’ intestines compared to the other treatments (p<0.05). Also, supplementation of 1 g or 2 g probiotic and 2 g antibiotic per liter of syrup for each hive decreased Escherichia coli counts in the intestine compared to the other treatments (p<0.05). The adding 1 g or 2 g probiotic per liter of syrup for each hive increased the levels of fructose and glucose in honey compared to the other treatments (p<0.05). The supplementation of 1 g or 2 g probiotic per liter of syrup decreased sucrose in honey compared to the other treatments (p<0.05). The results of the present study suggest that probiotic might be used as a feed additive for increased honey quality and gut microbial flora improvement in honey bees.

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“…In addition to Apb. kunkeei , other microbial species associated with healthy bees were evaluated regarding their probiotic properties for bees, humans, and other animals [ 99 ].…”

Section: Fructobacilli As Beneficial Microbesmentioning

confidence: 99%

Bee-Associated Beneficial Microbes—Importance for Bees and for Humans

Todorov,

Alves,

Bueno

et al. 2024

Insects

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Bees are one of the best-known and, at the same time, perhaps the most enigmatic insects on our planet, known for their organization and social structure, being essential for the pollination of agricultural crops and several other plants, playing an essential role in food production and the balance of ecosystems, being associated with the production of high-value-added inputs, and a unique universe in relation to bees’ microbiota. In this review, we summarize information regarding on different varieties of bees, with emphasis on their specificity related to microbial variations. Noteworthy are fructophilic bacteria, a lesser-known bacterial group, which use fructose fermentation as their main source of energy, with some strains being closely related to bees’ health status. The beneficial properties of fructophilic bacteria may be extendable to humans and other animals as probiotics. In addition, their biotechnological potential may ease the development of new-generation antimicrobials with applications in biopreservation. The concept of “One Health” brings together fundamental and applied research with the aim of clarifying that the connections between the different components of ecosystems must be considered part of a mega-structure, with bees being an iconic example in that the healthy functionality of their microbiota is directly and indirectly related to agricultural production, bee health, quality of bee products, and the functional prosperity for humans and other animals. In fact, good health of bees is clearly related to the stable functionality of ecosystems and indirectly relates to humans’ wellbeing, a concept of the “One Health”.

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Honeybee Gut: Reservoir of Probiotic Bacteria

Cited by 3 publications

References 59 publications

In vitro probiotic and safety attributes of Bacillus spp. isolated from beebread, honey samples and digestive tract of honeybees Apis mellifera

In vitro probiotic and safety attributes of Bacillus spp. isolated from beebread, honey samples and digestive tract of honeybees Apis mellifera

Comparative effect of probiotic and antibiotic on honey bees colony functional traits

Bee-Associated Beneficial Microbes—Importance for Bees and for Humans

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