Honeybee gut Lactobacillus modulates host learning and memory behaviors via regulating tryptophan metabolism

Zhang, Zijing; Mu, Xiaohuan; Cao, Qina; Shi, Yiting; Hu, Xiaosong; Zheng, Hao

doi:10.1038/s41467-022-29760-0

Cited by 101 publications

(74 citation statements)

References 72 publications

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“…Besides, the results of this study showed that lactic acid bacteria were significantly enriched in the midgut of V. v. nigrithorax males. Recent studies show that Lactobacillus Firm-5 species enhances bees' memory and honeybee gut Lactobacillus modulates host learning and memory behaviors via regulating tryptophan metabolism (Li et al, 2021;Zhang et al, 2022). Besides, Bifidobacterium spp.…”

Section: Discussionmentioning

confidence: 99%

Midgut Bacterial Communities of Vespa velutina Lepeletier (Hymenoptera: Vespidae)

Zhang

Liu²,

Wang

et al. 2022

Front. Ecol. Evol.

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Vespa velutina nigrithorax and Vespa velutina auraria are two subspecies of Vespa velutina Lepeletier. V. velutina preys managed honey bees, other pollinators, and insects. However, the Vespa midgut microbiota of three forms, namely queens, workers, and males have not been reported, thus the objective of this study was to analyze the midgut bacterial diversity of the three forms of V. v. nigrithorax and V. v. auraria. Our results showed that Proteobacteria, Firmicutes, Bacteroidetes, Tenericutes, and Actinobacteria were the most abundant phyla, and Lactobacillus (17.21%) and Sphingomonas (11.39%) were the most abundant genera in the midgut of V. v. nigrithorax and V. v. auraria. We found that the midgut bacterial compositions of the V. velutina males were special, in terms of richness and diversity of bacterial communities, as well as the content of lactic acid bacteria. By comparing the gut bacterial compositions of Vespa from different regions (Japan, South Korea, Italy, and China), it was discovered that the gut bacterial compositions were very similar at the phylum and class level, and Gammaproteobacteria, Bacilli, and Alphaproteobacteria were the most abundant classes of bacteria and consistent in the genus Vespa. Besides, though Vespa from different regions had quite different gut bacterial communities at the genus level, Lactobacillus and other lactic acid bacteria were abundant and played important roles in protection and metabolism in V. velutina collected from different regions. This is the first report of midgut bacterial diversity of three forms queens, workers, and males of V. velutina. Our findings provide insight that Proteobacteria and Firmicutes (especially Lactobacillus and other lactic acid bacteria) are consistent and may play important roles in the genus Vespa. The understanding of the microbiome in the midgut of Vespa and the discovery of the vital bacteria would provide useful information to design pest biological control agents. Thus, the significance of this study is to provide a basis for the study of the relationship between gut microbiota and physiology and health of Vespa, as well as the control of Vespa.

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Section: Discussionmentioning

confidence: 99%

Midgut Bacterial Communities of Vespa velutina Lepeletier (Hymenoptera: Vespidae)

Zhang

Liu²,

Wang

et al. 2022

Front. Ecol. Evol.

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“…Fisetin could improve hyperuricemia-induced chronic kidney disease via regulating the gut microbiota-mediated Trp metabolism [ 57 ]. Interestingly, honeybee gut lactobacillus modulates host learning and memory behaviors via regulating the Trp metabolism [ 175 ].…”

Section: Potential Application Of Gut Microbiota-derived Trp Metaboli...mentioning

confidence: 99%

Gut Microbiota-Derived Tryptophan Metabolites Maintain Gut and Systemic Homeostasis

Gao

Yang

2022

Cells

130

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Tryptophan is an essential amino acid from dietary proteins. It can be metabolized into different metabolites in both the gut microbiota and tissue cells. Tryptophan metabolites such as indole-3-lactate (ILA), indole-3-acrylate (IAC), indole-3-propionate (IPA), indole-3-aldehyde (IAID), indoleacetic acid (IAA), indole-3-acetaldehyde and Kyn can be produced by intestinal microorganisms through direct Trp transformation and also, partly, the kynurenine (Kyn) pathway. These metabolites play a critical role in maintaining the homeostasis of the gut and systematic immunity and also potentially affect the occurrence and development of diseases such as inflammatory bowel diseases, tumors, obesity and metabolic syndrome, diseases in the nervous system, infectious diseases, vascular inflammation and cardiovascular diseases and hepatic fibrosis. They can not only promote the differentiation and function of anti-inflammatory macrophages, Treg cells, CD4+CD8αα+ regulatory cells, IL-10+ and/or IL-35+B regulatory cells but also IL-22-producing innate lymphoid cells 3 (ILC3), which are involved in maintaining the gut mucosal homeostasis. These findings have important consequences in the immunotherapy against tumor and other immune-associated diseases. We will summarize here the recent advances in understanding the generation and regulation of tryptophan metabolites in the gut microbiota, the role of gut microbiota-derived tryptophan metabolites in different immune cells, the occurrence and development of diseases and immunotherapy against immune-associated diseases.

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“…In the KEGG pathway enrichment analysis (p < 0.05 Figure 2C) between the WT and cKO groups, the biological processes mainly included protein metabolism (protein digestion and absorption), glucose metabolism (glycolysis/gluconeogenesis, carbohydrate digestion, and absorption), and other types of metabolism (methane, tryptophan, and carbon) and hormone regulation (thyroid hormone synthesis). These biological processes directly or indirectly regulate learning and memory (Knezovic et al, 2018;Chaalal et al, 2019;Ajoy et al, 2021;Zhang et al, 2022). Moreover, three downregulated DEGs (Atf4 , t = .…”

Section: Altered Signaling Pathways and Expression Profiles Of Downst...mentioning

confidence: 99%

Conditional deletion of MAD2B in forebrain neurons enhances hippocampus-dependent learning and memory in mice

Su²,

Zhi³

et al. 2022

Front. Cell. Neurosci.

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Mitotic arrest deficient 2-like protein 2 (MAD2B) is not only a DNA damage repair agent but also a cell cycle regulator that is widely expressed in the hippocampus and the cerebral cortex. However, the functions of MAD2B in hippocampal and cerebral cortical neurons are poorly understood. In this study, we crossed MAD2Bflox/flox and calcium/calmodulin-dependent protein kinase II alpha (Camk2a)-Cre mice to conditionally knock out MAD2B in the forebrain pyramidal neurons by the Cre/loxP recombinase system. First, RNA sequencing suggested that the differentially expressed genes in the hippocampus and the cerebral cortex between the WT and the MAD2B cKO mice were related to learning and memory. Then, the results of behavioral tests, including the Morris water maze test, the novel object recognition test, and the contextual fear conditioning experiment, suggested that the learning and memory abilities of the MAD2B cKO mice had improved. Moreover, conditional knockout of MAD2B increased the number of neurons without affecting the number of glial cells in the hippocampal CA1 and the cerebral cortex. At the same time, the number of doublecortin-positive (DCX+) cells was increased in the dentate gyrus (DG) of the MAD2B cKO mice. In addition, as shown by Golgi staining, the MAD2B cKO mice had more mushroom-like and long-like spines than the WT mice. Transmission electron microscopy (TEM) revealed that spine synapses increased and shaft synapses decreased in the CA1 of the MAD2B cKO mice. Taken together, our findings indicated that MAD2B plays an essential role in regulating learning and memory.

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Honeybee gut Lactobacillus modulates host learning and memory behaviors via regulating tryptophan metabolism

Cited by 101 publications

References 72 publications

Midgut Bacterial Communities of Vespa velutina Lepeletier (Hymenoptera: Vespidae)

Midgut Bacterial Communities of Vespa velutina Lepeletier (Hymenoptera: Vespidae)

Gut Microbiota-Derived Tryptophan Metabolites Maintain Gut and Systemic Homeostasis

Conditional deletion of MAD2B in forebrain neurons enhances hippocampus-dependent learning and memory in mice

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