Honey-sensitive Pseudomonas aeruginosa mutants are impaired in catalase A

Bolognese, Fabrizio; Bistoletti, Michela; Barbieri, Paola; Orlandi, Viviana Teresa

doi:10.1099/mic.0.000351

Cited by 6 publications

(4 citation statements)

References 35 publications

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“…Our data suggesting that manuka honey affects quorum sensing via the downregulation of the pqsABCDE operon supports previous studies in P. aeruginosa PAO1 (35). Complementary techniques, such as microarray analysis, genetic screens, and proteomic approaches (27,(43)(44)(45), have shown honey can affect the expression of genes involved in the oxidative stress responses in S. aureus and E. coli, and our findings indicate that this also occurs in P. aeruginosa.…”

Section: Resultssupporting

confidence: 91%

Characterizing the Mechanism of Action of an Ancient Antimicrobial, Manuka Honey, against Pseudomonas aeruginosa Using Modern Transcriptomics

Bouzo

Cokcetin

et al. 2020

mSystems

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Manuka honey has broad-spectrum antimicrobial activity, and unlike traditional antibiotics, resistance to its killing effects has not been reported. However, its mechanism of action remains unclear. Here, we investigated the mechanism of action of manuka honey and its key antibacterial components using a transcriptomic approach in a model organism, Pseudomonas aeruginosa. We show that no single component of honey can account for its total antimicrobial action, and that honey affects the expression of genes in the SOS response, oxidative damage, and quorum sensing. Manuka honey uniquely affects genes involved in the explosive cell lysis process and in maintaining the electron transport chain, causing protons to leak across membranes and collapsing the proton motive force, and it induces membrane depolarization and permeabilization in P. aeruginosa. These data indicate that the activity of manuka honey comes from multiple mechanisms of action that do not engender bacterial resistance. IMPORTANCE The threat of antimicrobial resistance to human health has prompted interest in complex, natural products with antimicrobial activity. Honey has been an effective topical wound treatment throughout history, predominantly due to its broad-spectrum antimicrobial activity. Unlike traditional antibiotics, honey-resistant bacteria have not been reported; however, honey remains underutilized in the clinic in part due to a lack of understanding of its mechanism of action. Here, we demonstrate that honey affects multiple processes in bacteria, and this is not explained by its major antibacterial components. Honey also uniquely affects bacterial membranes, and this can be exploited for combination therapy with antibiotics that are otherwise ineffective on their own. We argue that honey should be included as part of the current array of wound treatments due to its effective antibacterial activity that does not promote resistance in bacteria.

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Section: Resultssupporting

confidence: 91%

Characterizing the Mechanism of Action of an Ancient Antimicrobial, Manuka Honey, against Pseudomonas aeruginosa Using Modern Transcriptomics

Bouzo

Cokcetin

et al. 2020

mSystems

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“…Regarding the feasibility of safeguarding the inter-lot reproducibility of a honey-based product, the use of honey with a single floral source, as happens with manuka honey (at least 70% of its pollen content should come from Leptospermum scoparium ) seems to be more convenient. Besides, the chestnut honey has already been reported to have high antimicrobial effect against E. coli (Coniglio et al, 2013 ) and, together with Manuka honey, against P. aeruginosa including PAO1 (Hao et al, 2012 ; Voncina et al, 2015 ; Bolognese et al, 2016 ). The tissue of chestnut plants contains compounds such as tannins and antioxidants (Hao et al, 2012 ), which have inhibitory effects on microorganisms, and 3-aminoacetophenone is the main volatile compound occurring specially in this floral source, known as having antibacterial properties (Bonaga and Giumanini, 1986 ).…”

Section: Discussionmentioning

confidence: 99%

Chestnut Honey and Bacteriophage Application to Control Pseudomonas aeruginosa and Escherichia coli Biofilms: Evaluation in an ex vivo Wound Model

et al. 2018

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Chronic skin wounds represent a major burn both economically and socially. Pseudomonas aeruginosa and Escherichia coli are among the most common colonizers of infected wounds and are prolific biofilm formers. Biofilms are a major problem in infections due to their increasingly difficult control and eradication, and tolerance to multiple prescribed drugs. As so, alternative methods are necessary. Bacteriophages (phages) and honey are both seen as a promising approach for biofilm related infections. Phages have specificity toward a bacterial genus, species or even strain, self-replicating nature, and avoid dysbiosis. Honey has gained acknowledgment due to its antibacterial, antioxidant and anti-inflammatory and wound healing properties. In this work, the effect of E. coli and P. aeruginosa phages vB_EcoS_CEB_EC3a and vB_PaeP_PAO1-D and chestnut honey, alone and combined, were tested using in vitro (polystyrene) and ex vivo (porcine skin) models and against mono and dual-species biofilms of these bacteria. In general, colonization was higher in the porcine skins and the presence of a second microorganism in a consortium of species did not affect the effectiveness of the treatments. The antibacterial effect of combined therapy against dual-species biofilms led to bacterial reductions that were greater for biofilms formed on polystyrene than on skin. Monospecies biofilms of E. coli were better destroyed with phages and honey than P. aeruginosa monospecies biofilms. Overall, the combined phage-honey formulations resulted in higher efficacies possibly due to honey's capacity to damage the bacterial cell membrane and also to its ability to penetrate the biofilm matrix, promoting and enhancing the subsequent phage infection.

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“…The roles of catalase and hydrogen peroxide in honey are demonstrated utilizing catalase deficient mutants (katA) against the Pseudomonas aeruginosa. 7 Many researchers have reported that natural unheated honey possesses potent antibacterial, anti-inflammatory, anti-ulcer and antioxidant activities which show beneficial effects against several diseases. [8][9][10][11] Usage of Honey in Dental and Oral Diseases Honey is implicated in the treatment of dental and oral diseases.…”

Section: Introductionmentioning

confidence: 99%

“…6 The hydrogen peroxide is further converted to water and oxygen by another enzyme catalase. 7 The trace minerals are the building blocks of tooth enamel. 23 Honey as a complementary and alternative medicine (termed as "Apitherapy") 31 has been used for centuries to treat following dental and oral diseases:…”

Section: Introductionmentioning

confidence: 99%

Honey: A Natural Remedy in Dental and Oral Diseases

I¹,

Sharma²,

Ahmad³

2018

SynsDoc

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Honey is a natural medicine that has anti-bacterial, anti-inflammatory, anti-ulcer and antioxidant properties. The medicinal value of honey is attributed to several components including hygroscopic sugars, and enzymes like diastase, invertase, catalase and glucose oxidase. Glucose oxidase catalyzes the oxidation of glucose to hydrogen peroxide and gluconolactone. The hydrogen peroxide is further converted to water and oxygen by enzyme catalase. The low moisture content and high viscosity of honey provide protective barrier against bacterial infection. Honey not only has an excellent nutritional value but it is also used as an ointment or a drug as a complementary and alternative medicine called "Apitherapy". This mini review presents the medicinal value of honey in the treatment of dental and oral diseases/conditions such as oral ulcer, oral candidiasis, stomatitis, dental carries, plaque, gingivitis, periodontal disease and orthodontic treatment, and tooth extraction.

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Honey-sensitive Pseudomonas aeruginosa mutants are impaired in catalase A

Cited by 6 publications

References 35 publications

Characterizing the Mechanism of Action of an Ancient Antimicrobial, Manuka Honey, against Pseudomonas aeruginosa Using Modern Transcriptomics

Characterizing the Mechanism of Action of an Ancient Antimicrobial, Manuka Honey, against Pseudomonas aeruginosa Using Modern Transcriptomics

Chestnut Honey and Bacteriophage Application to Control Pseudomonas aeruginosa and Escherichia coli Biofilms: Evaluation in an ex vivo Wound Model

Honey: A Natural Remedy in Dental and Oral Diseases

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