Honey prevents neurobehavioural deficit and oxidative stress induced by lead acetate exposure in male wistar rats- a preliminary study

Abstract

This research sought to investigate the possible neuroprotective effects of honey against lead (Pb)-induced neurotoxicity. Twenty four male Wistar rats were divided into four groups: Control group that received 1 ml/kg distilled orally for 28 days; while groups II-IV received 0.2% lead in drinking water and 1 ml/kg of distilled water, 1 ml/kg of honey, 1.5 ml/kg of honey respectively for 28 days. Anxiety and exploratory activities were determined in the open field test. Memory function was determined using Morris water maze after which the animals were sacrificed. The brains were then excised, homogenized and Lipid peroxidation (MDA), Superoxide dismutase (SOD), Catalase, Glutathione (GSH) and Glutathione -S- Transferase (GST) activities were determined in the brains. Results showed that lead exposure causes decrease in locomotor and exploratory activities; increase anxiety, memory impairment, lipid peroxidation and decrease antioxidant activities. However, co-administration of honey with lead inhibited neurotoxicity as indicated by the improvement in memory function as evidenced by decreased latency period and increased in time spent in target quadrant in honey-fed rats compared to the lead-exposed animals. Furthermore, honey increased locomotion, exploration and decreased anxiety in lead-exposed rats as indicated by the frequency of rearing, freezing duration and the number of line crossed by animals. Also administration of honey improves antioxidant activities as shown by increased brain SOD, GST and GSH activities compared to the lead-treated groups but no significant effect on MDA level. It can be concluded that honey has neuroprotective effects against lead-induced cognitive deficit probably by enhancing antioxidant activities.

In vivo immunoprotective role of Indigofera tinctoria and Scoparia dulcis aqueous extracts against chronic noise stress induced immune abnormalities in Wistar albino rats

“…It means that both extracts have ability to increase the antioxidant capacity to alleviate the effect of noise stress. Our results comparable with Abdul majeed et al [58] reported that honey (1.5 mL/kg b.w) decrease the lipid peroxidation level and enhanced the other antioxidant enzyme level on lead induced oxidative stress in wistar rat.…”

Honey on Brain Health: Benefit or Forfeit?

“…While both the tests for open field and Morris water showed results of positive neuroprotection effects of the honey, its MDA results showed no significant change between the groups. The results of antioxidant activities (catalase, SOD and GSH) however coincides with the results from the study by Azman et al, 2018 showing a significant increase in honey treated animals with the rats given 1.5 mL/kg honey better results compared to the lower dosed honey (Azman et al 2018;Abdulmajeed et al 2016). In another study, stinglees bee honey significantly increases lymphoblastic cell viability by more than 150%.…”

“…Results have mostly led to an increase of the free radicals causing this imbalanced condition. Honey have shown to attenuate with damage resulting from oxidative stress (Sairazi et al 2017;Ranneh et al 2019;Azman et al 2018;Abdulmajeed et al 2016).…”

“…Another recent study tested the neuroprotective effects of honey against lead (Pb)-induced neurotoxicity (Abdulmajeed et al 2016). All rats were given 0.2% Pb in drinking water and amongst them were groups given honey (1 mL/kg and 1.5 mL/kg separately).…”

Phytochemistry, metabolism, and ethnomedical scenario of honey: A concurrent review

“…[127,128] Organic compounds having antioxidant and free radical scavenging activity in honey composition shows that it keeps the ability to work in human nutrition as a vital source of antioxidants. [129] Besides, as far as antiinflammatory effect of honey is concerned, it causes a drop in necrosis tissues.…”