Honey/Chitosan Nanofiber Wound Dressing Enriched with Allium sativum and Cleome droserifolia: Enhanced Antimicrobial and Wound Healing Activity

Abstract

Two natural extracts were loaded within fabricated honey, poly(vinyl alcohol), chitosan nanofibers (HPCS) to develop biocompatible antimicrobial nanofibrous wound dressing. The dried aqueous extract of Cleome droserifolia (CE) and Allium sativum aqueous extract (AE) and their combination were loaded within the HPCS nanofibers in the HPCS-CE, HPCS-AE, and HPCS-AE/CE nanofiber mats, respectively. It was observed that the addition of AE resulted in the least fiber diameter (145 nm), whereas the addition of the AE and CE combination resulted in the least swelling ability and the highest weight loss. In vitro antibacterial testing against Staphylococcus aureus, Escherichia coli, Methicillin-resistant S. aureus (MRSA), and multidrug-resistant Pseudomonas aeruginosa was performed in comparison with the commercial dressing AquacelAg and revealed that the HPCS-AE and HPCS-AE/CE nanofiber mats allowed complete inhibition of S. aureus and the HPCS-AE/CE exhibited mild antibacterial activity against MRSA. A preliminary in vivo study revealed that the developed nanofiber mats enhanced the wound healing process as compared to the untreated control as proved by the enhanced wound closure rates in mice and by the histological examination of the wounds. Moreover, comparison with the commercial dressing Aquacel Ag, the HPCS, and HPCS-AE/CE demonstrated similar effects on the wound healing process, whereas the HPCS/AE allowed an enhanced wound closure rate. Cell culture studies proved the biocompatibility of the developed nanofiber mats in comparison with the commercial Aquacel Ag, which exhibited noticeable cytotoxicity. The developed natural nanofiber mats hold potential as promising biocompatible antibacterial wound dressing.

Nanosilver coated Chitin derived membrane as an Eco-friendly Dressing and Its Effect on Wound Healing

“…This may be related to some characteristics of chitosan and nanosilver, for example, they are all safe, and non-toxic, so that they can be widely used in the food industry [15,16]. the wound healing rates of the Control, A, B, C and D groups respectively were 18.5 %, 24.6 %, 30.1 %, 37.8 % and 41.3 % after 3 days of injury, and 35.2 %, 45.8 %, 56.7 %, 67.9 % and 73.8% respectively after 7 days of injury.…”

Electrospinning of natural polymers for advanced wound care: towards responsive and adaptive dressings

“…Sarhan et al have produced honey/chitosan/PVA fibres loaded with Cleome droserifolia (CE) and Allium sativum extracts. 99 Together with the ability to stop the formation of S. aureus biofilms, the composite dressings demonstrated high levels of fibroblasts viability and enhanced wound repair in vivo, when compared with the commercial product Aquacel. Hybrid and multilayers systems have been also developed for promoting cells proliferation and healing.…”

“…Recently, composite chitosan fibres with enhanced antibacterial properties have been prepared using natural bioactive compounds, such as essential oils and honey. [97][98][99][100] Cinnamon oil has been incorporated into chitosan/poly(ethylene oxide) (PEO) fibres having a diameter of around 50 nm. 97 The electrospun mats were able to release the essential oil in vitro, and exhibited antibacterial activity against E. coli and P. aeruginosa.…”

Review: Multimodal bioactive material approaches for wound healing

“…86 Other alternative treatments have also been explored, including the impact of honey on infection prevention. 87,88 These strategies that look beyond the typical silver and antibiotic based treatments provide potential alternatives to combat antibiotic resistance.…”