Honey bee gut microbial communities are robust to the fungicide Pristine® consumed in pollen

DeGrandi-Hoffman, Gloria; Corby‐Harris, Vanessa; deJong, Emily Watkins; Chambers, Mona; Hidalgo, Geoffrey

doi:10.1007/s13592-016-0478-y

Cited by 24 publications

(15 citation statements)

References 57 publications

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“…This result somewhat agrees with past work that showed that pollen provision-associated bacteria do not affect fungi, while fungi may increase the diversity of the bacterial community through a currently unknown mechanism [62]. Conversely, DeGrandi-Hoffman et al [70] found that honey bee exposure to fungicides did not affect their microbiomes. While our results are insightful, they do not indicate if the bees are picking up live microbes, or if there is any effect on bee health or metabolism.…”

Section: Discussionsupporting

confidence: 92%

Floral and Foliar Source Affect the Bee Nest Microbial Community

et al. 2018

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Managed pollinators such as the alfalfa leafcutting bee, Megachile rotundata, are essential to the production of a wide variety of agricultural crops. These pollinators encounter a diverse array of microbes when foraging for food and nest-building materials on various plants. To test the hypothesis that food and nest-building source affects the composition of the bee-nest microbiome, we exposed M. rotundata adults to treatments that varied both floral and foliar source in a 2 × 2 factorial design. We used 16S rRNA gene and internal transcribed spacer (ITS) sequencing to capture the bacterial and fungal diversity of the bee nests. We found that nest microbial communities were significantly different between treatments, indicating that bee nests become inoculated with environmentally derived microbes. We did not find evidence of interactions between the fungi and bacteria within our samples. Furthermore, both the bacterial and fungal communities were quite diverse and contained numerous exact sequence variants (ESVs) of known plant and bee pathogens that differed based on treatment. Our research indicates that bees deposit plantassociated microbes into their nests, including multiple plant pathogens such as smut fungi and bacteria that cause blight and wilt. The presence of plant pathogens in larval pollen provisions highlights the potential for bee nests to act as disease reservoirs across seasons. We therefore suggest that future research should investigate the ability of bees to transmit pathogens from nest to host plant.

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Section: Discussionsupporting

confidence: 92%

Floral and Foliar Source Affect the Bee Nest Microbial Community

et al. 2018

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“…A wealth of information on dysbiosis comes from a one month confinement of colonies to small flight enclosures [21]. Indoor colonies quickly decline and often develop disease.…”

Section: Gut Dysbiosis (Complete Displacement)mentioning

confidence: 99%

“…A separate confinement experiment produced a completely different form of dysbiosis, characterized by a total lack of native gut bacteria in adults [21]. The core bacteria were displaced by specialized larval pathogen Melissococcus plutonius, the causative agent of European foulbrood, and associated secondary opportunists Enterococcus faecalis and Paenibacillus alvei [50].…”

Section: Gut Dysbiosis (Complete Displacement)mentioning

confidence: 99%

Honey bee gut dysbiosis: a novel context of disease ecology

Anderson

Ricigliano

2017

Current Opinion in Insect Science

111

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The honey bee microbiota has become a hot-spot of recent research. Highly co-evolved with its host, the hindgut microbiota of a worker honey bee consists of six bacterial species shown to occur reliably in particular proportions. Altered microbiota structure is associated with host deficiencies, and a variety of bacteria found throughout the hive environment can dominate the worker gut suppressing or displacing microbiota function. The synthesis presented here suggests environmental insults alter gut bacterial balance, leading to decreased host function and disease progression. Specific functional groups of native bacteria represent a model system to investigate dysbiosis and the evolution of host tolerance/resistance traits in honey bee-microbe interactions.

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“…Hypopharyngeal glands were dissected from 5 bees per cage on day 7 and from five nurse bees from colonies after one full brood cycle of consuming the diet treatments. Bees were flash frozen in liquid nitrogen, and maintained at − 80°C until their HPG were measured using previously described techniques (DeGrandi-Hoffman et al 2010, 2017. Briefly, HPG were removed from head capsules and placed into a PBS buffer (37 mM NaCl, 2.7 mM KCl, and 10 mM PO4, pH 7.4).…”

Section: Hypopharyngeal Glandsmentioning

confidence: 99%

Effects of diets containing different concentrations of pollen and pollen substitutes on physiology, Nosema burden, and virus titers in the honey bee (Apis mellifera L.)

Jong

DeGrandi-Hoffman

Chen³

et al. 2019

Apidologie

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Colonies of Apis mellifera provided with natural forage show decreased pathogen loads and increased overwintering success when compared with colonies provisioned with supplemental protein diets. Despite the potential benefits of a pollen-based diet, protein supplements are commonly used in colonies throughout the spring and increasingly through the fall and winter as the cost of pollen is greater than that of supplements and concerns exist over the potential for pollen to vector viruses to bee colonies. In this study, we compare virus and Nosema burden, consumption and digestion, hemolymph protein and hypopharyngeal gland size in bees fed on pure pollen diets, purely supplemental protein diets, and diets containing 90% supplement and 10% pollen to examine whether the inclusion of small amounts of pollen mitigates the negative impacts of consuming protein supplements seen in preceding studies. We found that the diets had similar concentrations of total soluble protein; however, bees consumed and digested significantly more of the pollen diet than either PS alone or mixed with pollen. In colonies, honeybees consuming pollen had lower deformed wing virus (DWV) and Nosema titers than bees fed protein supplements. The addition of 10% pollen to the supplement significantly increased digestion and hypopharyngeal gland size and decreased levels of Nosema infection over colonies fed supplement alone. These results indicate that the addition of small amounts of pollen into protein supplements may help to mitigate the differences observed between protein supplement and pollen fed bees. honey bee nutrition / Nosema / deformed wing virus / hypopharyngeal gland / supplementary feeding

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Honey bee gut microbial communities are robust to the fungicide Pristine® consumed in pollen

Cited by 24 publications

References 57 publications

Floral and Foliar Source Affect the Bee Nest Microbial Community

Floral and Foliar Source Affect the Bee Nest Microbial Community

Honey bee gut dysbiosis: a novel context of disease ecology

Effects of diets containing different concentrations of pollen and pollen substitutes on physiology, Nosema burden, and virus titers in the honey bee (Apis mellifera L.)

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