Homovanillic Acid, 3,4‐dihydroxyphenylacetic Acid and 5‐hydroxyindol‐3‐ylacetic Acid in Serial Samples of Cerebrospinal Fluid From the Lateral Ventricle of the Dog

Ashcroft, G. W.; Crawford, T. B. B.; Dow, R. C.; Guldberg, H. C.

doi:10.1111/j.1476-5381.1968.tb00493.x

Cited by 162 publications

(38 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The concentrations of 5-HIAA and homovanillic acid rose above control concentrations and almost reached values which have been found for endogenous concentrations of these acids in perfusion experiments with dogs treated with probenecid (Ashcroft et al, 1968a) where the active transport mechanism has been shown to be inhibited. Despite the absence of detectable phenylacetic acid in brain, these results support the hypothesis that part of the action of pheneizine in blocking the egress of 5-HIAA from brain and c.s.f.…”

Section: Phenylacetic Acidmentioning

confidence: 85%

“…5-HIAA concentrations in c.s.f. were measured by the method of Ashcroft et al (1968a). Phenelzine and phenylacetic acid did not interfere with any of the above analyses.…”

Section: Biochemical Methodologymentioning

confidence: 99%

“…in the dog has been shown to be located in the region of the fourth ventricle (Ashcroft et al, 1968a) and is presumably responsible for the marked gradient of 5-HIAA and homovanillic acid from lateral ventricle to cisterna magna. Thus the action of phenelzine which caused an inhibition of this localized transport mechanism explains why lowered concentrations of 5-HIAA were found in the c.s.f.…”

Section: Brain Analyses Following Administration Of Tryptophan or Salinementioning

confidence: 99%

“…It has been shown previously (Ashcroft et al, 1968a) that inulin clearance represents the bulk removal of fluid from the c.s.f. system and that a high proportion of low concentrations of 5-HIAA and homovanillic acid are cleared from c.s.f.…”

Section: Brain Analyses Following Administration Of Tryptophan or Salinementioning

confidence: 99%

See 3 more Smart Citations

Interaction in the cerebral metabolism of the biogenic amines. Effects of phenelzine on the cerebral metabolism of the 5‐hydroxyindoles in dog brain

Moir

1972

British J Pharmacology

View full text Add to dashboard Cite

Summary Chronic administration of phenelzine to dogs caused 5‐hydroxyindol‐3‐ylacetic acid (5‐HIAA) concentrations in c.s.f. from the lateral ventricle to be maintained at 50% of their normal values, but did not alter the concentrations of 5‐HIAA in c.s.f. from the cisterna magna. Following 10 days treatment with phenelzine, the active transport of 5‐HIAA from c.s.f. which normally occurs in the region of the fourth ventricle, was inhibited. This transport system was also inhibited by the addition of phenylacetic acid, the acid metabolite of phenelzine, to the fluid perfusing the cerebral ventricles. After 10–12 days treatment with phenelzine all regions of brain showed concentration increases to approximately 300% for 5‐HT and 150% for 5‐HIAA, but no alteration in the tryptophan concentration. Intravenous administration of tryptophan to dogs pretreated with phenelzine caused large increases in the concentration of tryptophan in brain and body fluids but did not alter either the concentrations of 5‐hydroxytryptamine and 5‐HIAA in brain or of 5‐HIAA in c.s.f. A model of the cerebral metabolism of 5‐hydroxytryptamine is proposed and the results are interpreted to mean that phenelzine has inhibitory actions, either directly or in some instances indirectly, on intracerebral tryptophan 5‐hydroxylase, monoamine oxidase and the transport of 5‐HIAA from both brain and c.s.f.

show abstract

Section: Phenylacetic Acidmentioning

confidence: 85%

“…5-HIAA concentrations in c.s.f. were measured by the method of Ashcroft et al (1968a). Phenelzine and phenylacetic acid did not interfere with any of the above analyses.…”

Section: Biochemical Methodologymentioning

confidence: 99%

Section: Brain Analyses Following Administration Of Tryptophan or Salinementioning

confidence: 99%

Section: Brain Analyses Following Administration Of Tryptophan or Salinementioning

confidence: 99%

See 2 more Smart Citations

Interaction in the cerebral metabolism of the biogenic amines. Effects of phenelzine on the cerebral metabolism of the 5‐hydroxyindoles in dog brain

Moir

1972

British J Pharmacology

View full text Add to dashboard Cite

show abstract

“…is demonstrated in this paper, using a method (Ashcroft, Crawford, Dow & Guldberg, 1968) of repeated sampling and analyses of lateral ventricular C.S.F. in dogs.…”

mentioning

confidence: 99%

Effects of chlorpromazine on the metabolism of catecholamines in dog brain

Guldberg

Yates

1969

British J Pharmacology

Self Cite

View full text Add to dashboard Cite

1. The effect of chlorpromazine (CPZ) on the metabolism of dopamine and 5-hydroxytryptamine in dog brain was investigated by following the concentrations of the acid metabolites of these amines, homovanillic acid, 3,4-dihydroxyphenylacetic acid and 5-hydroxyindolylacetic acid, in the ventricular cerebrospinal fluid (C.S.F.) of dogs over a period of 5 hr after intravenous administration of CPZ (2'5, 5, 10 and 15 mg/kg), using the technique of serial sampling of lateral ventricular C.S.F. "Low" doses (25-10 mg/kg) produced a rise in the concentration of homovanillic acid and smaller increases in the concentrations of 3,4-dihydroxyphenylacetic acid and 5-hydroxyindolylacetic acid. "High" doses (10-15 mg/kg) had a lesser effect on the concentration of homovanillic acid and had no effect on, or decreased, the concentrations of 3,4-dihydroxyphenylacetic acid and 5-hydroxyindolylacetic acid. The concentration of 3,4-dihydroxyphenylacetic acid was maximal in the ventricular C.S.F. 2 hr after CPZ 5 mg/kg and was unaltered from the control level 2 hr after 15 mg/kg. 2. The effects on the metaibolism of brain amines of CPZ (5 mg/kg), doses which the serial sampling of C.S.F. experiments had indicated as producing maximal and minimal effects on dopamine metabolism in brain tissue, were studied by estimating the concentrations of adrenaline, noradrenaline, dopamine, metanephrine, methoxydopamine, homovanillic acid, 3,4-dihydroxyphenylacetic acid and 5-hydroxyindolylacetic acid in the hypothalamus, midbrain, thalamus, hindbrain, cortex, globus pallidus and caudate nucleus of control dogs and of dogs treated with CPZ intravenously 2 hr before killing. The concentrations of homovanillic acid, 3,4-dihydroxyphenylacetic acid and 5-hydroxyindolylacetic acid were estimated in samples of ventricular C.S.F. withdrawn from these dogs 2 hr after the injection of CPZ (i.e., immediately before death).3. The following changes in concentrations were observed. Dopamine: CPZ 5 mg/kg produced no change in the concentration in the caudate nucleus, globus pallidus and midbrain and increased the concentration in the thalamus; CPZ 15 mg/kg appeared to cause a reduction in the concentration of this amine in the caudate nucleus and globus pallidus. Homovanillic acid and 3,4-dihydroxyphenylacetic acid: CPZ 5 mg/kg increased the concentrations of both acids in the caudate nucleus and had no effect on the concentrations * Present address:

show abstract