Homotypic aggregation of murine B lymphocytes is independent of CD23

Davey, Edward J.; Bartlett, W C; Kikutani, Hitoshi; Fujiwara, Hiroshi; Kishimoto, Tadamitsu; Conrad, Daniel H.; Severinson, Eva

doi:10.1002/eji.1830250514

Cited by 20 publications

(14 citation statements)

References 36 publications

(19 reference statements)

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“…We have previously shown that CD2 is involved in aggregation of activated B cells [9]. On the other hand, there is evidence that CD23 is not an adhesion molecule in this system [14]. CD23 does not function as a compensatory adhesion molecule in LFA-1 -/-B cells because anti-CD23 had no effect on adhesion of these cells.…”

Section: Discussionmentioning

confidence: 92%

Assessment of the Role of Leucocyte Function‐Associated Antigen‐1 in Homotypic Adhesion of Activated B Lymphocytes

1998

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Greicius G, Tamosiunas V, Severinson E. Assessment of the Role of Leucocyte Function-Associated Antigen-1 in Homotypic Adhesion of Activated B Lymphocytes. Scand J Immunol 1998;48:642-650 In this study we investigated how T-cell-dependent stimuli, via interleukin-4 (IL-4) or CD40 ligation, influence homotypic B-cell adhesion when compared with induction by the T-cell-independent stimulus lipopolysaccharide (LPS). Using primary murine B cells, we found that T-cell-dependent stimulation led to increased aggregation as compared to that induced by LPS. The adhesion was to a large extent dependent on the adhesion molecule, lymphocyte function-associated antigen-1 (LFA-1). We found that activation of B cells with the mitogenic stimuli induced an increased avidity of LFA-1 for its ligand, intercellular adhesion molecule-1 (ICAM-1). The increase was stable and different from that induced by phorbol esters. Although adhesion was reduced using B cells from LFA-1 -/-mice, aggregation occurred in response to T-celldependent stimuli. Our data suggest that adhesion of B lymphocytes is regulated in different modes. One is induced by antigen and leads to a transient conformational change of the LFA-1 molecule. Another is induced by mitogenic stimuli and leads to stable avidity increase of LFA-1, possibly via activation of cytoskeletal anchorage. A third is LFA-1 independent, of low avidity and is induced by T-cell-dependent stimuli.

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Section: Discussionmentioning

confidence: 92%

Assessment of the Role of Leucocyte Function‐Associated Antigen‐1 in Homotypic Adhesion of Activated B Lymphocytes

1998

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“…[41][42][43] Recently, CD21 has been shown to mediate homotypic and heterotypic cell binding of normal B cells and B cell lines through adhesion to its ligand, CD23. [44][45][46] Since anti-CD21 but not anti-CD23 impaired the anti-CD19-induced aggregation of B-CLL cells, CD21 appears to employ a ligand different from CD23 for this homotypic adhesion. Treatment with anti-CD19 did not lead to an increased expression of the relevant adhesion molecules.…”

Section: Discussionmentioning

confidence: 99%

Differential adhesion pattern of B cell chronic lymphocytic leukemia cells

Behr

Korinth²,

Schriever³

1998

Leukemia

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“…The first two alternatives are unlikely to be involved. Although human CD23 has been shown to interact with CR2, influencing B cell activation [84], no such interaction has been observed in mice [85]. This is probably due to the low amino acid homology between the part of human CD23 shown to interact with human CD21 and the corresponding part of murine CD23 [86].…”

Section: Enhancement Of Antibody Responses By Ige/antigen Complexesmentioning

confidence: 94%