Homotransplantation Of Canine Trachea After Denervation Of The Spleen: A Preliminary Report

Toivio, I; Siirala, Urpo; Lauerma, Sirkka; Rapo, S.; Tallberg, T; Mahlberg, Kaj; Tapaninen, J.; Meurala, H

doi:10.3109/00016487309139686

Cited by 10 publications

(5 citation statements)

References 3 publications

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“…No immunosuppressive medications were given because of previous graft immunosterilization with formaldehyde and thimerosal. 11,20 Postoperative pain was managed with intramuscular injections of buprenorphine hydrochloride, 0.15 mg/kg.…”

Section: Methodsmentioning

confidence: 99%

“…16,17 Synthetic grafts have been investigated, but these are uniformly unsuccessful be-cause of the problem of infection. 18 Other animal studies [19][20][21] have investigated microvascular grafting, cryopreservation, and homografting after denervation of the spleen. Summarized, the results of these studies show that it is possible to transplant homograft tracheae into pigs, dogs, rabbits, and humans; however, unless the MHC markers are removed, or the recipient is immunosuppressed, rejection will occur.…”

Section: Original Articlementioning

confidence: 99%

“…Cryopreservation, irradiation, and chemical preservation effectively remove the MHC markers and allow airway transplantation without immunosuppression. 16,17,19,20,22,23 We are presently following up a 6-year-old child who received the first human tracheal transplant for longsegment tracheal stenosis as an infant (Figure 1). The graft has been in place for almost 5 years, and the child has minimal respiratory symptoms; however, endoscopy reveals little evidence of tracheal growth, and the transplanted segment is malacic (Figure 2).…”

Section: Original Articlementioning

confidence: 99%

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Growth and Development of Homograft Tracheal Transplants in the Piglet Model

Faulkner

Mair²

2001

Arch Otolaryngol Head Neck Surg

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Section: Methodsmentioning

confidence: 99%

Section: Original Articlementioning

confidence: 99%

Section: Original Articlementioning

confidence: 99%

See 1 more Smart Citation

Growth and Development of Homograft Tracheal Transplants in the Piglet Model

Faulkner

Mair²

2001

Arch Otolaryngol Head Neck Surg

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“…Other immunosuppression methods include denervation of the spleen, 47 high dose radiation therapy, 48 photodynamic therapy, 49 medication (Cialit and Merthiolate, 50 and t-octylphenoxypolyethoxyethanol 51 ) used in the treatment of reduction of graft antigenicity, or interleukin-10 gene transfer therapy to restrain an immune response of T cells. 52 It is necessary to wait for future reports on the long-term results of those methods.…”

Section: Rejection Reaction and Immunosuppressionmentioning

confidence: 99%

Cryopreservation of the tracheal grafts

Nakanishi

2009

Organogenesis

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Transplantation of the trachea may become the preferred method for the reconstruction of extensive tracheal defects, however, several unresolved problems must be addressed, such as immunosuppression, preservation and donor shortage. In this manuscript, the cryopreservation of tracheal grafts is reviewed, which potentially is associated with a lessened immunological response. Cryopreservation may be used clinically for long-term preservation and may solve the donor shortage. It is very important to confirm the immunomodulatory effect of cryopreservation on tracheal allografts in order to expand the potential clinical application of tracheal transplantation in the future. The cartilage as well as the epithelium and lamina propria serve as targets for rejection. However, the effect of cryopreservation on chondrocytes could be associated with reduced allogenicity of the trachea. The long-term cryopreservation of cartilage must be investigated in basic research models of chondrocyte viability. Growth of cryopreserved tracheal allografts is less well understood. Further studies are needed to elucidate the mechanism of synergistic effects of both cryopreservation and adequate immunosuppression for tracheal xenografts.

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“…Toivio et al (1973) performed allotransplantation of the trachea in adult mongrel dogs and observed lymphocyte aggregation at the site of collapse of tracheal cartilage and perivascular lymphocyte cuffing generally distributed in the subepithelial space. Lane et al (1977) transplanted allogeneic tracheas to the abdominal walls of rats and confirmed the resulting rejection reaction ; normal epithelium of the graft was maintained for several days, mononuclear cells consisting principally of small lymphocytes appeared in the epithelium or perivascular sites in the subepithelial layer 10 to 15 days after surgery, and subsequently, activated lymphocytes and lymphoblasts induced tissue injuries.…”

Section: Discussionmentioning

confidence: 99%

Experimental Tracheal Allotransplantation Using Omentopexy: Histological Process of Rejection Reaction without Immunosuppression.

Moriyama

Seimizu

Teramoto

1992

Tohoku J. Exp. Med.

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A large defect in the thoracic trachea was produced by rejecting 10 rings of tracheal cartilage in each of 11 adult mongrel dogs. An allograft of the five tracheal rings was interposed in this site via end-to-end anastomosis and then wrapped with the pedicled omental flap. These animals received no immunosuppressive therapy. The pseudostratified tracheal epithelia were remained and the graft revascularization through the omental pedicle proved to be established within about one week after surgery without immunosuppression. Marked mononuclear cell infiltration was observed beneath the epithelia and around the capillaries in the subepithelial layer over one week after surgery, which was followed by phagocytosis of the tracheal cartilages by macrophages. After the completion of rejection reaction, inflammation finally induced scarring or necrosis of the tracheal allograft, resulting in asphyxia or perforation. Only one animal survived 71 days after surgery without graft trouble. However, even in this animal, weak rejection reaction was observed microscopically and the structure of the graft was not that of the normal trachea. We think these results suggest that allotransplantation of trachea induces rejection, and therefore, that the use of immunosuppressants is necessary.

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Homotransplantation Of Canine Trachea After Denervation Of The Spleen: A Preliminary Report

Cited by 10 publications

References 3 publications

Growth and Development of Homograft Tracheal Transplants in the Piglet Model

Growth and Development of Homograft Tracheal Transplants in the Piglet Model

Cryopreservation of the tracheal grafts

Experimental Tracheal Allotransplantation Using Omentopexy: Histological Process of Rejection Reaction without Immunosuppression.

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