Homotransplant of pelvic ganglion into bladder wall in adult rats

Gabella, Giorgio; Uvelius, Bengt

doi:10.1016/s0306-4522(97)00401-6

Cited by 16 publications

(12 citation statements)

References 18 publications

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“…It also indicated that the efferent motor branches of somatic nerves can grow into the parasympathetic fibers of autonomic nerves through axonal regeneration, similar to the results of de Castro et al [14] and Eugene et al [15] . In the new reflex, somatic motor excitation evoked by the cutaneous stimuli is probably transmitted to the bladder through those anastomosed efferent motor nerves, resulting in the autonomic bladder contraction.…”

Section: Discussionsupporting

confidence: 84%

Morphological changes of cholinergic nerve fibers in the urinary bladder after establishment of artificial somatic-autonomic reflex arc in rats

Wang

Wen

et al. 2007

Neurosci. Bull.

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ObjectiveTo establish an artificial somatic-autonomic reflex arc in rats and observe the following distributive changes of neural fibers in the bladder. Methods Adult Sprague-Dawley rats were randomly divided into three groups: control group, spinal cord injury (SCI) group, and reinnervation group. DiI retrograde tracing was used to verify establishment of the model and to investigate the transport function of the regenerated efferent axons in the new reflex arc. Choline acetyltransferase (ChAT) in the DiI-labeled neurons was detected by immunohistochemistry. Distribution of neural fibers in the bladder was observed by acetylcholine esterase staining. Results DiI-labeled neurons distributed mainly in the left ventral horn from L3 to L5, and some of them were also ChAT-positive. The neural fibers in the bladder detrusor reduced remarkably in the SCI group compared with the control (P < 0.05). After establishment of the somatic-autonomic reflex arc in the reinnervation group, the number of ipsilateral fibers in the bladder increased markedly compared with the SCI group (P < 0.05), though still much less than that in the control (P < 0.05). Conclusion The efferent branches of the somatic nerves may grow and replace the parasympathetic preganglionic axons through axonal regeneration. Acetylcholine is still the major neurotransmitter of the new reflex arc. The controllability of detrusor may be promoted when it is reinnervated by the pelvic ganglia efferent somatic motor fibers from the postganglionic axons.

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Section: Discussionsupporting

confidence: 84%

Morphological changes of cholinergic nerve fibers in the urinary bladder after establishment of artificial somatic-autonomic reflex arc in rats

Wang

Wen

et al. 2007

Neurosci. Bull.

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“…The nerve cell bodies of the contralateral pelvic ganglion undergo hypertrophy, in connection with the increased target organ mass [16]. Normally, the rat bladder is devoid of intramural ganglion cells [17,18]. After unilateral denervation, small new ganglion cells appear along the axon-free nerves [17].…”

Section: Discussionmentioning

confidence: 99%

“…When a portion of the pelvic ganglion in the rat is transplanted into the denervated side of the bladder [18], nerves grow out of the transplant and connect with the original intramural nerves of the bladder. Also, many thin nerve bundles spread out of the grafted ganglion into the musculature.…”

Section: Discussionmentioning

confidence: 99%

Nerve distribution in rat urinary bladder after incorporation of acellular matrix graft or subtotal cystectomy

Frederiksen

Davidsson

Gabella

et al. 2008

Scandinavian Journal of Urology and Nephrology

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“…An increased production of nerve growth factor (NGF) by the hypertrophic bladder muscle is a major factor in the growth in size of the nerve cell body (Steers et al 1991). Most of the neurons return to the control size when the hypertrophy is reversed (Gabella and Uvelius 1998).…”

Section: Nerve Cell Sizementioning

confidence: 99%

Estimate of size and total number of neurons in superior cervical ganglion of rat, capybara and horse

2004

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The superior (cranial) cervical ganglion was investigated by light microscopy in adult rats, capybaras (Hydrochaeris hydrochaeris) and horses. The ganglia were vascularly perfused, embedded in resin and cut into semi-thin sections. An unbiased stereological procedure (disector method) was used to estimate ganglion neuron size, total number of ganglion neurons, neuronal density. The volume of the ganglion was 0.5 mm3 in rats, 226 mm3 in capybaras and 412 mm3 in horses. The total number of neurons per ganglion was 18,800, 1,520,000 and 3,390,000 and the number of neurons per cubic millimetre was 36,700, 7,000 and 8,250 in rats, capybaras and horses, respectively. The average neuronal size (area of the largest sectional profile of a neuron) was 358, 982 and 800 microm2, and the percentage of volume occupied by neurons was 33, 21 and 17% in rats, capybaras and horses, respectively. When comparing the three species (average body weight: 200 g, 40 kg and 200 kg), most of the neuronal quantitative parameters change in line with the variation of body weight. However, the average neuronal size in the capybara deviates from this pattern in being larger than that of in the horse. The rat presented great interindividual variability in all the neuronal parameters. From the data in the literature and our new findings in the capybara and horse, we conclude that some correlations exist between average size of neurons and body size and between total number of neurons and body size. However, these correlations are only approximate and are based on averaged parameters for large populations of neurons: they are less likely to be valid if one considers a single quantitative parameter. Several quantitative features of the nervous tissue have to be taken into account together, rather than individually, when evolutionary trends related to size are considered.

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Homotransplant of pelvic ganglion into bladder wall in adult rats

Cited by 16 publications

References 18 publications

Morphological changes of cholinergic nerve fibers in the urinary bladder after establishment of artificial somatic-autonomic reflex arc in rats

Morphological changes of cholinergic nerve fibers in the urinary bladder after establishment of artificial somatic-autonomic reflex arc in rats

Nerve distribution in rat urinary bladder after incorporation of acellular matrix graft or subtotal cystectomy

Estimate of size and total number of neurons in superior cervical ganglion of rat, capybara and horse

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