Homology between mitochondrial DNA of Agaricus bisporus and an internal portion of a linear mitochondrial plasmid of Agaricus bitorquis

Robison, Mary M.; Royer, John C.; Horgen, Paul A.

doi:10.1007/bf00312742

Cited by 59 publications

(34 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2b), we refrained from cloning other fragments of the plasmid. The high AT content of 73% is consistent with data obtained for many other linear plasmids of fungi, such as 74% for the pEM of Agaricus bitorquis (Robison et al, 1991), 67% for the pMC3-2 of Morchella conica (Rohe et al, 1991) and 70% for the kalilo of Neurospora intermedia (Chan et al, 1991).…”

Section: Isolation Of Nucleic Acidssupporting

confidence: 84%

“…Identical residues in the five polypeptides are indicated with asterisks . Source of plasmid sequences (EMBL Heidelberg, Germany): H. circinans pHC2 [this work], Agaricus bitorquis pEM (Robison et al, 1991), Ascobolus immersus pAl2 (Kempken et al, 1989), Morchella conica pMC3-2 (Rohe et al, 1991), Claviceps purpurea pCLK1 (Oeser and Tudzynski 1989), Claviceps purpurea pCLT5 (Oeser et al, 1993), Neurospora intermedia kalilo (Chan et al, 1991), Neurospora crassa maranhar (Court and Bertrand, 1992), Podospora anserina pAL2-1 (Hermanns and Osiewacz,1992), Gelasinospora sp. Gel-kaIDNA (Yuewang et al, 1996). cleavage sites given in the map (Fig.…”

Section: Isolation Of Nucleic Acidsmentioning

confidence: 99%

“…Comparative sequence analyses have revealed phylogenetic relations among several linear elements, such as adenoviruses, phages with linear genomes, cytoplasmic killer plasmids of yeasts, and the mitochondria) linear plasmids of filamentous fungi (Griffiths, 1995;Kempken, 1995;Meinhardt et SLI., 1990;Schrunder and Meinhardt, 1995). Linear plasmids have been very rarely described in basidiomycetes and, up to now, for only one element, pEM of Agaricus bitorquis (Robison et al, 1991), is sequence data available. The objective of this contribution was to broaden our knowledge about plasmids from basidiomycetes.…”

mentioning

confidence: 99%

See 2 more Smart Citations

The ectomycorrhizal basidiomycete Hebeloma circinans harbors a linear plasmid encoding a DNA- and RNA polymerase.

Debaud

Schründer

et al. 1997

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

Bulk DNA isolated from the ectomycorrhizal basidiomycete Hebe/oma circinans was treated with proteinase K and submitted to agarose gel electrophoresis.In addition to high molecular weight genomic DNA, three minor bands were detected. The band with the highest electrophoretic mobility (2.2 kbp) corresponds to double-stranded RNA. The two other bands, termed pHC1 and pHC2, were shown to be dsDNA molecules of 10.3 and 9.1 kbp, respectively. Treatment of the pHC elements with 3'-and 5'-specific exonucleases revealed a linear structure and proved that the 5' ends are protected from digestion; for pHC2, linearity was confirmed by restriction mapping. A 3.2 kbp Hindlll fragment of pHC2 was cloned and sequenced; it contains two open reading frames encoding putative viral B type DNA and RNA polymerases. Thus, the fungus harbors a typical linear plasmid, up to now, rarely described for basidiomycetes and hitherto unknown for mycorrhizal species.

show abstract

Section: Isolation Of Nucleic Acidssupporting

confidence: 84%

Section: Isolation Of Nucleic Acidsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

The ectomycorrhizal basidiomycete Hebeloma circinans harbors a linear plasmid encoding a DNA- and RNA polymerase.

Debaud

Schründer

et al. 1997

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

show abstract

“…We have searched available sequence databases for other examples of split T7-like RNAPs. A number of examples of fragmented, probably inactive enzymes in mitochondrial genomes of fungi (21,51) and Beta vulgaris (7) have been described. Others were found by us in the Spizellomyces punctatus mitochondrial chromosome sequence.…”

Section: Discussionmentioning

confidence: 99%

N4 RNA Polymerase II, a Heterodimeric RNA Polymerase with Homology to the Single-Subunit Family of RNA Polymerases

Willis¹,

Kazmierczak²,

Carter

et al. 2002

J Bacteriol

View full text Add to dashboard Cite

Bacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4 early promoter Pe3. These genes code for proteins of 269 and 404 amino acid residues with sequence similarity to the single-subunit, phage-like RNA polymerases. The genes encoding the N4 RNAPII subunits, as well as a synthetic construct encoding a fusion polypeptide, have been cloned and expressed. Both the individually expressed subunits and the fusion polypeptide reconstitute functional enzymes in vivo and in vitro.Sequence and structural analysis of DNA-dependent RNA polymerases (RNAPs) conclusively supports the existence of two enzyme families. One family consists of the large, evolutionarily related, multisubunit polymerases of eubacteria, archaea, and the nuclear and chloroplast polymerases of eukaryotes (reference 56 and references therein). Single-subunit enzymes with sequence homology to the T3/T7 phage polymerases belong to the second family of DNA-dependent RNAPs (38).Transcription of coliphage N4 middle mRNAs requires the activities of three early proteins: p17, p7, and p4 (53,70,72). Two of these gene products, p7 (30 kDa) and p4 (40 kDa), are soluble, have been purified to homogeneity, and constitute a heterodimeric, rifampin-resistant RNAP, N4 RNAPII (71). However, this heterodimer does not transcribe promoter-containing, double-stranded DNAs (dsDNA) and utilizes singlestranded DNAs (ssDNA) with low efficiency and no specificity. At least one additional phage-coded protein, p17, is essential in vivo for N4 middle RNA synthesis but is not sufficient in vitro for utilization of N4 middle promoters (1, 70; R. H. Carter, unpublished data). Recent in vitro characterization of p17 indicates that p17 is a ssDNA binding protein that recruits N4 RNAPII to ssDNA templates (R. H. Carter and A. Demidenko, unpublished data).In the present study, the genes encoding the p7 and p4 subunits of N4 RNAPII were sequenced and cloned. Both genes are transcribed from the same phage early promoter. The two subunits display sequence similarity to separate, nonoverlapping regions of single-subunit, T7-like RNAPs, suggesting that gene fusion or gene splitting events have occurred during the evolution of this class of polymerases. An N4 fusion polypeptide was generated that is active in vitro and complements phages carrying mutations in either of the two subunits when expressed in vivo. Ϫ m K12 ϩ recA1) was used in the construction of expression vectors. Protein expression was carried out in either BL21(DE3) (62) or W3350(DE3)pLysS (11). Phage were grown as described previously (53). MATERIALS AND METHODS Bacterial strains and phages. Escherichia coliPreparation and manipulation of DNAs. N4 DNA was prepared according to the work of VanderLaan et al. (64). DNA amplification reaction mixtures contained 1 g of tem...

show abstract

“…A reverse transcriptase activity has been proposed to explain the formation and replication of the Mauriceville homology group plasmids (12), while the LaBelle and Fiji plasmids were found to encode a DNA-dependent DNA polymerase (13) like other linear plasmids such as kalilo from Neurospora intermedia (14), maranhar from Neurospora crassa (15), pEM from Agaricus bitorquis (16), pClK1 from Claviceps purpurea (17), pAI2 from Ascobolus immersus (18) and S1 from maize (19).…”

Section: Introductionmentioning

confidence: 99%

The Cryphonectria parasitica plasmid pUG1 contains a large ORF with motifs characteristic of family B DNA polymerases

Gobbi

Carpanelli

Firrao

et al. 1997

Nucleic Acids Research

View full text Add to dashboard Cite

The isolation and characterization of the circular mitochondrial plasmid pUG1 from the ascomycete Cryphonectria parasitica is described. The entire sequence (4182 bp) was obtained and high similarities to DNA-dependent DNA polymerases were revealed. Strikingly common features with the DNA polymerases encoded by the Neurospora intermedia plasmids Fiji and LaBelle, such as matches to the conserved motifs A and B and the presence of TTD instead of DTD in motif C, were found, suggesting the existence of a distinct group of members of the B DNA family polymerases. These strong similarities between the plasmids might suggest a common origin of the C.parasitica and the Neurospora plasmids.

show abstract

Homology between mitochondrial DNA of Agaricus bisporus and an internal portion of a linear mitochondrial plasmid of Agaricus bitorquis

Cited by 59 publications

References 32 publications

The ectomycorrhizal basidiomycete Hebeloma circinans harbors a linear plasmid encoding a DNA- and RNA polymerase.

The ectomycorrhizal basidiomycete Hebeloma circinans harbors a linear plasmid encoding a DNA- and RNA polymerase.

N4 RNA Polymerase II, a Heterodimeric RNA Polymerase with Homology to the Single-Subunit Family of RNA Polymerases

The Cryphonectria parasitica plasmid pUG1 contains a large ORF with motifs characteristic of family B DNA polymerases

Contact Info

Product

Resources

About