Homologues of the <i>Arabidopsis thaliana SHI/STY/LRP1</i> genes control auxin biosynthesis and affect growth and development in the moss <i>Physcomitrella patens</i>

Eklund, D. Magnus; Thelander, Mattias; Landberg, Katarina; Ståldal, Veronika; Nilsson, Anders; Johansson, Monika; Valsecchi, Isabel; Pederson, Eric; Kowalczyk, Mariusz; Ljung, Karin; Ronne, Hans; Sundberg, Eva

doi:10.1242/dev.039594

Cited by 87 publications

(82 citation statements)

References 60 publications

(86 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown previously, SHI/STY family members form two clades supported by high bootstrap values Eklund et al, 2010b). We identified two main types of conserved GCC box-containing elements in SHI/STY members of Arabidopsis.…”

Section: A Gcc Box In Upstream Regulatory Regions Of Shi/sty Members supporting

confidence: 77%

“…The three Selaginella moelendorffii and the two Physcomitrella patens SHI/STY homologs cluster with the LRP1/SRS6 genes (Eklund et al, 2010b). Several SHI/STY family members in Arabidopsis form evolutionarily closely related pairs, most likely originating from the last genome duplication event .…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, promoters of the two P. patens SHI/STY orthologs PpSHI1 and PpSHI2 (Eklund et al, 2010b) were not found to possess GCC box-like sequences. Likewise, GCC boxlike elements could not be found in the lycophyte SHI/ STY genes (data not shown).…”

Section: Gcc Boxes Are Only Found In Shi/sty Family Promoters Of Angimentioning

confidence: 99%

See 2 more Smart Citations

Expression of ArabidopsisSHORT INTERNODES/STYLISHFamily Genes in Auxin Biosynthesis Zones of Aerial Organs Is Dependent on a GCC Box-Like Regulatory Element

et al. 2011

Self Cite

View full text Add to dashboard Cite

Auxin/indole-3-acetic acid (IAA) biosynthesis in Arabidopsis (Arabidopsis thaliana) plays a major role in growth responses to developmental and genetic signals as well as to environmental stimuli. Knowledge of its regulation, however, remains rudimentary, and few proteins acting as transcriptional modulators of auxin biosynthesis have been identified. We have previously shown that alteration in the expression level of the SHORT INTERNODES/STYLISH (SHI/STY) family member STY1 affects IAA biosynthesis rates and IAA levels and that STY1 acts as a transcriptional activator of genes encoding auxin biosynthesis enzymes. Here, we have analyzed the upstream regulation of SHI/STY family members to gain further insight into transcriptional regulation of auxin biosynthesis. We attempted to modulate the normal expression pattern of STY1 by mutating a putative regulatory element, a GCC box, located in the proximal promoter region and conserved in most SHI/STY genes in Arabidopsis. Mutations in the GCC box abolish expression in aerial organs of the adult plant. We also show that induction of the transcriptional activator DORNRÖ SCHEN-LIKE (DRNL) activates the transcription of STY1 and other SHI/STY family members and that this activation is dependent on a functional GCC box. Additionally, STY1 expression in the strong drnl-2 mutant or the drn drnl-1 puchi-1 triple mutant, carrying knockdown mutations in both DRNL and its close paralogue DRN as well as one of their closest homologs, PUCHI, was significantly reduced, suggesting that DRNL regulates STY1 during normal plant development and that several other genes might have redundant functions.

show abstract

Section: A Gcc Box In Upstream Regulatory Regions Of Shi/sty Members supporting

confidence: 77%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Expression of ArabidopsisSHORT INTERNODES/STYLISHFamily Genes in Auxin Biosynthesis Zones of Aerial Organs Is Dependent on a GCC Box-Like Regulatory Element

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…This indicates that in the moss, auxin plays a role in development and that the components of auxin signalling operate, at least to some extent. Recently, Eklund et al (2010) provided further confirmation of auxin action in P. patens when they showed that the A. thaliana SHI/STY gene family (positive regulators of auxin biosynthesis genes) also regulate auxin levels, and affect growth and development in the moss. These observations are consistent with the suggestion that land plants acquired functional or potentially functional components of auxin signalling before the divergence of the bryophyte groups from the lineage leading to vascular plants, but after the algae diverged from that lineage (Lau et al 2009).…”

Section: Auxin Signallingmentioning

confidence: 96%

“…Recently, Eklund et al (2010) suggested that the tryptamine and indole-3-pyruvic acid pathways might operate in Physcomitrella patens, based on the presence of genes usually associated with these pathways (YUCCA genes for the tryptamine pathway and TAA1-like genes for the indole-3-pyruvic acid pathway).…”

Section: Introductionmentioning

confidence: 99%

Evolution of growth-promoting plant hormones

Ross

Reid

2010

Functional Plant Biol.

View full text Add to dashboard Cite

Abstract. The plant growth hormones auxin, gibberellins (GAs) and brassinosteroids (BRs) are major determinants of plant growth and development. Recently, key signalling components for these hormones have been identified in vascular plants and, at least for the GAs and BRs, biosynthetic pathways have been clarified. The genome sequencing of a range of species, including a few non-flowering plants, has allowed insight into the evolution of the hormone systems. It appears that the moss Physcomitrella patens can respond to auxin and contains key elements of the auxin signalling pathway, although there is some doubt as to whether it shows a fully developed rapid auxin response. On the other hand, P. patens does not show a GA response, even though it contains genes for components of GA signalling. The GA response system appears to be more advanced in the lycophyte Selaginella moellendorffii than in P. patens. Signalling systems for BRs probably arose after the evolutionary divergence of the mosses and vascular plants, although detailed information is limited. Certainly, the processes affected by the growth hormones (e.g. GAs) can differ in the different plant groups, and there is evidence that with the evolution of the angiosperms, the hormone systems have become more complex at the gene level. The intermediate nature of mosses in terms of overall hormone biology allows us to speculate about the possible relationship between the evolution of plant growth hormones and the evolution of terrestrial vascular plants in general.

show abstract

Chiral events in developing gametophores of Physcomitrella patens and other moss species are driven by an unknown, universal direction‐sensing mechanism

Zagórska‐Marek

Sokołowska

Turzańska

2018

American J of Botany

View full text Add to dashboard Cite

Premise of the Study We used the model species Physcomitrella patens to examine chirality in moss gametophores. Chirality is manifested in the direction of consecutive apical cell divisions, cell plate configurations, and deviations of leaf connecting lines from the vertical course. However, the frequencies of chiral configurations of all these processes as well as their mutual dependence—especially in the case of gametophore branching—are not known. Other moss species were checked to determine the universality of our findings. Methods The gametophore structure of Physcomitrella patens grown in the laboratory under controlled conditions was investigated using light microscopy and compared with that of other moss species collected from their natural stands. Key Results In all investigated moss species, the tetrahedral apical cell exhibits either clockwise or counterclockwise consecutive divisions, and selection of this directionality in the primary axis is random. It is, however, related to cell plate configuration. If the plate is skewed, leaf‐producing segments arising from the apical cell cleavage exhibit circumferential rotation. Three parallel lines connecting the leaves deviate from a vertical course, but always in the same direction as that of leaf initiation; thus, the angular distance between consecutive leaves increases to >120°. Lateral branches are exclusively antidromous. Conclusions Gametophore chiral configuration appears to be useful in resolving problems of moss modular growth and branching. Morphological and anatomical evidence strongly suggests that an unknown direction‐sensing mechanism controls the development of moss axial organs. We propose that leaves are responsible for a horizontal gradient of sugar signals that develops along the gametophore circumference, thus influencing branching‐unit chirality.

show abstract

Homologues of the Arabidopsis thaliana SHI/STY/LRP1 genes control auxin biosynthesis and affect growth and development in the moss Physcomitrella patens

Cited by 87 publications

References 60 publications

Expression of ArabidopsisSHORT INTERNODES/STYLISHFamily Genes in Auxin Biosynthesis Zones of Aerial Organs Is Dependent on a GCC Box-Like Regulatory Element

Expression of ArabidopsisSHORT INTERNODES/STYLISHFamily Genes in Auxin Biosynthesis Zones of Aerial Organs Is Dependent on a GCC Box-Like Regulatory Element

Evolution of growth-promoting plant hormones

Chiral events in developing gametophores of Physcomitrella patens and other moss species are driven by an unknown, universal direction‐sensing mechanism

Contact Info

Product

Resources

About