Homologs of the rolC gene of naturally transgenic toadflaxes Linaria vulgaris and Linaria creticola are expressed in vitro

Matveeva, T. V.; Bogomaz, Olesja Denisovna; Golovanova, L. A.; Li, Yu. S.; Dimitrov, Dimitar

doi:10.18699/vj18.359

Cited by 4 publications

(1 citation statement)

References 15 publications

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“…Opine synthesis genes make up the majority of cT-DNAs described to date [16,18], and it is the opine synthesis genes that often remain intact. Their expression was detected in the roots as well as in the shoots [8,14,31,42]. Moreover, detectable levels of DFG, mikimopine, and agrocinopine have been found in N. tabacum [31], Cuscuta suaveolens [17], and transgenic tobacco with the I. batatas acs gene [33], respectively.…”

Section: Discussionmentioning

confidence: 99%

Nicotiana noctiflora Hook. Genome Contains Two Cellular T-DNAs with Functional Genes

Khafizova,

Sierro,

Ivanov

et al. 2023

Plants

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Agrobacterium (Rhizobium)-mediated transformation leads to the formation of crown galls or hairy roots on infected plants. These effects develop due to the activity of T-DNA genes, gathered on a big plasmid, acquired from agrobacteria during horizontal gene transfer. However, a lot of plant species are known to contain such sequences, called cellular T-DNAs (cT-DNAs), and maintain normal phenotypes. Some of the genes remain intact, which leads to the conclusion of their functional role in plants. In this study, we present a comprehensive analysis of the cT-DNAs in the Nicotiana noctiflora Hook. genome, including gene expression and opine identification. Deep sequencing of the Nicotiana noctiflora genome revealed the presence of two different cT-DNAs, NnT-DNA1 and NnT-DNA2, which contain the intact genes iaaM, iaaH, acs, orf13, orf13a, and orf14. According to the expression analysis results, all these genes are most active in roots in comparison with other organs, which is consistent with data on cT-DNA gene expression in other plant species. We also used genetic engineering approaches and HPTLC and HPLC-MS methods to investigate the product of the acs gene (agrocinopine synthase), which turned out to be similar to agrocinopine A. Overall, this study expands our knowledge of cT-DNAs in plants and brings us closer to understanding their possible functions. Further research of cT-DNAs in different species and their functional implications could contribute to advancements in plant genetics and potentially unveil novel traits with practical applications in agriculture and other fields.

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Section: Discussionmentioning

confidence: 99%

Nicotiana noctiflora Hook. Genome Contains Two Cellular T-DNAs with Functional Genes

Khafizova,

Sierro,

Ivanov

et al. 2023

Plants

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Agrobacterium-Mediated Transformation in the Evolution of Plants

Matveeva

2018

Current Topics in Microbiology and Immunology

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In most cases, the genetic engineering of plants uses Agrobacterium-mediated transformation to introduce novel genes. In nature, insertion of T-DNA into the plant genome and its subsequent transfer via sexual reproduction have been shown for several species in the genera Nicotiana, Ipomoea , and Linaria . A sequence homologous to T-DNA of the Ri plasmid of Agrobacterium rhizogenes was found in the genome of wild-type Nicotiana glauca (section Noctiflorae) more than 30 years ago and was named "cellular T-DNA" (cT-DNA). It comprises an imperfect inverted repeat and contains homologs of several T-DNA oncogenes (NgrolB, NgrolC, Ngorf13, Ngorf14) and an opine synthesis gene (Ngmis). Multiple cT-DNAs have also been found in species of the sections Tomentosae and Nicotiana of the genus Nicotiana. These ancient cT-DNA genes are still expressed, indicating that they may play a role in the evolution of these plants. In 2012-2013, cT-DNA was detected and characterized in Linaria vulgaris and L. genistifolia ssp. dalmatica. Their cT-DNA is present in two copies and organized as an imperfect direct tandem repeat, containing LvORF2, LvORF3, LvORF8, LvrolA, LvrolB, LvrolC, LvORF13, LvORF14, and the Lvmis genes. In 2015, cT-DNA was found in Ipomoea. Two types of T-DNA-like sequences were described within this genera, and their distribution varied among cultured hexaploid, tetraploid, and wild diploid forms. Thus, several independent T-DNA integration events occurred in the genomes of these three plant genera. We propose that the events of T-DNA insertion in the plant genome might have affected their evolution, resulting in the creation of new plant species. In this chapter, we focus on the structure and functions of cT-DNA in Linaria, Nicotiana, and Ipomoea and discuss their possible evolutionary role.

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Characterization and identification of naturally transgenic species Linaria vulgaris pathogenic mycromycetes

Сокорнова

Гасич

Bemova

et al. 2018

Ecological genetics

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In nature there are species containing homologs of T-DNA genes of agrobacteria (сT-DNA) in their genomes. Such plants are called naturally transgenic ones. Interaction with the microbiota is one of the possible functions of cT-DNA, discussed in the literature. Linaria plants are the most suitable for the investigation of the probable ecological role of T-DNA, since they widely spread. The first stage in the evaluation of plant-microbial interactions involving these plants is the description of isolates with contrasting virulence for toadflax. The search and DNA-barcoding of such isolates of Phoma-like fungi was the goal of this work. 14 strains isolated from the plants of the families Plantaginaceae and Scrophullariaceae were analyzed. The of multilocus analysis included amplification and sequencing of internal transcribed spacers, a large subunit of RNA, a tubulin gene. Based on molecular data, 9 strains were assigned to the species Boeremia exigua, which has a wide range of habitats and a wide specialization. Strains of this species were virulent against L. vulgaris, but differed in aggressiveness with respect to this plant. Thus, a collection of strains was characterized, which can later be used for a more detailed study of the immune response of the naturally-transgenic L. vulgaris plant in response to inoculation with the B. exigua phytopathogen. As a result of the work, we identified the narrow host range fungi Heterophoma novae-verbascicola, and broad host range pathogens Plectosphaerella cucumerina, Phoma herbarum and Trichothecium roseum. Among them, only P. cucumerina was a weak pathogen of L. vulgaris. These results confirm the early data on the depleted mycobiota of L. vulgaris.

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Homologs of the rolC gene of naturally transgenic toadflaxes Linaria vulgaris and Linaria creticola are expressed in vitro

Cited by 4 publications

References 15 publications

Nicotiana noctiflora Hook. Genome Contains Two Cellular T-DNAs with Functional Genes

Nicotiana noctiflora Hook. Genome Contains Two Cellular T-DNAs with Functional Genes

Agrobacterium-Mediated Transformation in the Evolution of Plants

Characterization and identification of naturally transgenic species Linaria vulgaris pathogenic mycromycetes

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