Homologous recombination in the archaea: the means justify the ends

White, Malcolm F.

doi:10.1042/bst0390015

Cited by 51 publications

(42 citation statements)

References 48 publications

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“…Strikingly, ⌬saci_1500 did not show increased sensitivity toward these agents compared to that of wild-type cells (Fig. 7), which is different from the findings for RecQ deletion mutants in the Eukarya (49)(50)(51)(52). Together these results suggest that the ATPase and unwinding activity of Saci_1500 is specifically involved in UV-related DNA damage repair.…”

Section: Figmentioning

confidence: 66%

“…In archaea, operon-encoded Mre11, Rad50, HerA, and NurA are responsible for DSB end resection during HR. However, little is known about archaeal proteins involved in HR processes other than end resection (52). In this study, we investigated the role of four genes (saci_1497 to saci_1500) downstream of the ups gene cluster encoding an endonuclease III, a ParB-like protein, a predicted glycosyltransferase, and a helicase, respectively.…”

Section: Discussionmentioning

confidence: 99%

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DNA Processing Proteins Involved in the UV-Induced Stress Response of Sulfolobales

Wolferen

Albers

2015

J Bacteriol

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The ups operon of Sulfolobus species is highly induced upon UV stress. Previous studies showed that the pili encoded by this operon are involved in cellular aggregation, which is essential for subsequent DNA exchange between cells, resulting in homologous recombination. The presence of this pilus system increases the fitness of Sulfolobus cells under UV light-induced stress conditions, as the transfer of DNA takes place in order to repair UV-induced DNA lesions via homologous recombination. Four conserved genes (saci_1497 to saci_1500) which encode proteins with putative DNA processing functions are present downstream of the ups operon. In this study, we show that after UV treatment the cellular aggregation of strains with saci_1497, saci_1498, and saci_1500 deletions is similar to that of wild-type strains; their survival rates, however, were reduced and similar to or lower than those of the pilus deletion strains, which could not aggregate anymore. DNA recombination assays indicated that saci_1498, encoding a ParB-like protein, plays an important role in DNA transfer. Moreover, biochemical analysis showed that the endonuclease III encoded by saci_1497 nicks UV-damaged DNA. In addition, RecQ-like helicase Saci_1500 is able to unwind homologous recombination intermediates, such as Holliday junctions. Interestingly, a saci_1500 deletion mutant was more sensitive to UV light but not to the replication-stalling agents hydroxyurea and methyl methanesulfonate, suggesting that Saci_1500 functions specifically in the UV damage pathway. Together these results suggest a role of Saci_1497 to Saci_1500 in the repair or transfer of DNA that takes place after UV-induced damage to the genomic DNA of Sulfolobus acidocaldarius. IMPORTANCESulfolobales species increase their fitness after UV stress by a UV-inducible pilus system that enables high rates of DNA exchange between cells. Downstream of the pilus operon, three genes that seem to play a role in the repair or transfer of the DNA between Sulfolobus cells were identified, and their possible functions are discussed. Next to the previously described role of UV-inducible pili in the exchange of DNA, we have thereby increased our knowledge of DNA transfer at the level of DNA processing. This paper therefore contributes to the overall understanding of the DNA exchange mechanism among Sulfolobales cells. In all domains of life, DNA repair is crucial for maintenance of genome integrity upon exposure to intra-or extracellular DNAdamaging threats (1). Unlike DNA repair in bacteria, archaeal DNA repair and its regulation are still far from well understood (1, 2). Homology searches revealed that the proteins in archaeal DNA repair pathways show similarities to both bacterial and eukaryotic proteins. In addition, DNA repair proteins with unique archaeal features exist (3).Previously, it was speculated that archaea might have SOS-like responses similar to those in certain bacteria (4); however, microarray studies revealed that neither Haloarchaea (5, 6) nor Sulfolobus species (7...

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Section: Figmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

DNA Processing Proteins Involved in the UV-Induced Stress Response of Sulfolobales

Wolferen

Albers

2015

J Bacteriol

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“…Then two RuvB Eco hexameric rings, recruited through contacts with RuvA Eco to the HJ, drive branch migration (11). Finally, binding of the junctionspecific endonuclease, RuvC Eco , leads to the formation of a RuvABC Eco complex with the potential dislodging of one RuvA Eco tetramer (6,12). The RuvABC Eco complex resolves HJs and completes the recombination process (13).…”

mentioning

confidence: 99%

“…From the results obtained with E. coli enzymes, it is believed that bacterial HJ resolvases work in concert with RuvAB. However, recent data from other bacteria suggest that the in vivo role of ubiquitous HJ translocases (RecG and RuvAB) might not be conserved: (i) the absence of both branch migration translocases, RecG and RuvAB, is synthetically lethal in B. subtilis (Firmicutes phylum) or Neisseria gonorrhoeae (representative of ␤-Proteobacteria class) (24 -26); (ii) ruvAB and recG show a synergistic defect in DNA repair in E. coli cells (␥-Proteobacteria class); (iii) recG suppresses the recombination defect of the ruvB mutations in Helicobacter pylori (representative of ⑀-Proteobacteria Class) (27); (iv) ruvAB is synthetically lethal in the recU context (24); (v) the resolvases from phages or Archaea seem to act independently of the presence of a branch migration helicase (6,12,28).…”

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confidence: 99%

Interaction of Branch Migration Translocases with the Holliday Junction-resolving Enzyme and Their Implications in Holliday Junction Resolution

Cañas

Suzuki

Marchisone

et al. 2014

Journal of Biological Chemistry

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Background: Bacillus subtilis RuvAB and RecG translocases and RecU resolvase are crucial in homologous recombination. Results: RecU interacts with RuvB and re-localizes it at a Holliday junction (HJ). RuvB stimulates RecU. RecG and RuvAB unwind HJs. Conclusion: A RecU⅐HJ⅐RuvAB complex might be formed. Significance: RecU, which interacts with RecA, may help to discriminate between RuvAB and RecG at HJs.

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“…In archaea, the picture is not clear. Homologous recombination (HR) is presumably the preferred process of DSBR as proteins involved in HR have been identified whereas Ku homologs have not (White, 2011); yet a recent genetic study on Haloferax volcanii demonstrate that DSBR by HR is restrained, likely because this species is highly polyploid (Delmas et al, 2009). Nevertheless, the study of such processes in hyperthermophilic archaea is of importance, given that they are exposed to DNA damaging temperatures and are among the most radioresistant organisms, repairing fragmented chromosomes efficiently (DiRuggiero et al, 1997;Gerard et al, 2001;Jolivet et al, 2003a;Jolivet et al, 2003b).…”

Section: The Mre11-rad50 Complex In Archaeamentioning

confidence: 99%