Homologous recombination in plants is temperature and day-length dependent

Boyko, Alexander; Filkowski, Jody; Kovalchuk, Igor

doi:10.1016/j.mrfmmm.2004.12.011

Cited by 53 publications

(39 citation statements)

References 44 publications

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“…Similarly, in contrast with Molinier et al (2005) and Yao et al (2011), who found a seven-or threefold increase in recombination frequency, respectively, as a result of UV-C irradiation in Arabidopsis, Van der Auwera et al (2008) did not find a significant effect of UV-C in their SHR lines containing GUS recombination reporter (Van der Auwera et al, 2008). Furthermore, in contrast with earlier results (Kovalchuk et al, 2001b;Boyko et al, 2005), Van der Auwera et al (2008) reported that the presence of heavy metals, such as lead or cadmium ions, a heat shock of 50˚C, or growth at elevated temperatures and increased daylength had no measurable effect on the mutation or recombination frequencies . In contrast with the reported 1.5-fold to sevenfold increased recombination level in two tested Arabidopsis lines as a reaction to spraying plants with the salicylic acid analogs INA and benzothiadiazole (Lucht et al, 2002), or the results of Yao et al (2011) who used methyl salicylate, Van der Auwera et al (2008) did not find a significant difference when they added sodium salicylate to the growth medium (Van der Auwera et al, 2008).…”

Section: Conflicting Results From Shr Reporter Linescontrasting

confidence: 76%

Reevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter Lines

et al. 2012

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A widely used approach for assessing genome instability in plants makes use of somatic homologous recombination (SHR) reporter lines. Here, we review the published characteristics and uses of SHR lines. We found a lack of detailed information on these lines and a lack of sufficient evidence that they report only homologous recombination. We postulate that instead of SHR, these lines might be reporting a number of alternative stress-induced stochastic events known to occur at transcriptional, posttranscriptional, and posttranslational levels. We conclude that the reliability and usefulness of the somatic homologous recombination reporter lines requires revision. Thus, more detailed information about these reporter lines is needed before they can be used with confidence to measure genome instability, including the complete sequences of SHR constructs, the genomic location of reporter genes and, importantly, molecular evidence that reconstituted gene expression in these lines is indeed a result of somatic recombination.

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Section: Conflicting Results From Shr Reporter Linescontrasting

confidence: 76%

Reevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter Lines

et al. 2012

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“…Both lines were exposed to an extended variety of abiotic stress types (Table 1). These include conditions previously shown to stimulate SHR in these and/or other SHR trap lines: salt, heat, cold, radiomimetic (bleocin) or oxidative (paraquat) drugs, UV-B and UV-C [6], [8]–[11], [13], [18], [23]–[26]. To test for a potential role of epigenetic factors in the control of SHR [19], [21], we further applied drugs previously not tested and affecting either DNA methylation [zebularine; 27] or histone modifications [trichostatin A, sodium butyrate; 28].…”

Section: Resultsmentioning

confidence: 99%

Transgenerational Stress Memory Is Not a General Response in Arabidopsis

et al. 2009

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Adverse conditions can trigger DNA damage as well as DNA repair responses in plants. A variety of stress factors are known to stimulate homologous recombination, the most accurate repair pathway, by increasing the concentration of necessary enzymatic components and the frequency of events. This effect has been reported to last into subsequent generations not exposed to the stress. To establish a basis for a genetic analysis of this transgenerational stress memory, a broad range of treatments was tested for quantitative effects on homologous recombination in the progeny. Several Arabidopsis lines, transgenic for well-established recombination traps, were exposed to 10 different physical and chemical stress treatments, and scored for the number of somatic homologous recombination (SHR) events in the treated generation as well as in the two subsequent generations that were not treated. These numbers were related to the expression level of genes involved in homologous recombination and repair. SHR was enhanced after the majority of treatments, confirming previous data and adding new effective stress types, especially interference with chromatin. Compounds that directly modify DNA stimulated SHR to values exceeding previously described induction rates, concomitant with an induction of genes involved in SHR. In spite of the significant stimulation in the stressed generations, the two subsequent non-treated generations only showed a low and stochastic increase in SHR that did not correlate with the degree of stimulation in the parental plants. Transcripts coding for SHR enzymes generally returned to pre-treatment levels in the progeny. Thus, transgenerational effects on SHR frequency are not a general response to abiotic stress in Arabidopsis and may require special conditions.

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“…If correct, this would implicate temperature as another possible mutagenic factor alongside UV-B. However, experiments with one of the same transgenic lines of Arabidopsis used by Ries et al (2000) fails to support this possibility, with only a 1.4-fold increase in HR events in response to a 118C temperature increase from 22 to 338C (Boyko et al 2005). Collectively, these data indicate that simulated increases in UV-B radiation (figure 7) might destabilize plant genomes to a significantly greater extent than any increases in temperature.…”

Section: (A ) Siberian Traps Versus Ch 3 CL Release From Rocksmentioning

confidence: 99%

The stability of the stratospheric ozone layer during the end-Permian eruption of the Siberian Traps

Beerling

Harfoot

Lomax

et al. 2007

Phil. Trans. R. Soc. A.

105

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The discovery of mutated palynomorphs in end-Permian rocks led to the hypothesis that the eruption of the Siberian Traps through older organic-rich sediments synthesized and released massive quantities of organohalogens, which caused widespread O 3 depletion and allowed increased terrestrial incidence of harmful ultraviolet-B radiation (UV-B, 280-315 nm; Visscher et al. 2004 Proc. Natl Acad. Sci. USA 101, 12 952-12 956). Here, we use an extended version of the Cambridge two-dimensional chemistry-transport model to evaluate quantitatively this possibility along with two other potential causes of O 3 loss at this time: (i) direct effects of HCl release by the Siberian Traps and (ii) the indirect release of organohalogens from dispersed organic matter. According to our simulations, CH 3 Cl released from the heating of coals alone caused comparatively minor O 3 depletion (5-20% maximum) because this mechanism fails to deliver sufficiently large amounts of Cl into the stratosphere. The unusual explosive nature of the Siberian Traps, combined with the direct release of large quantities of HCl, depleted the model O 3 layer in the high northern latitudes by 33-55%, given a main eruptive phase of less than or equal to 200 kyr. Nevertheless, O 3 depletion was most extensive when HCl release from the Siberian Traps was combined with massive CH 3 Cl release synthesized from a large reservoir of dispersed organic matter in Siberian rocks. This suite of model experiments produced column O 3 depletion of 70-85% and 55-80% in the high northern and southern latitudes, respectively, given eruption durations of 100-200 kyr. On longer eruption time scales of 400-600 kyr, corresponding O 3 depletion was 30-40% and 20-30%, respectively. Calculated year-round increases in total near-surface biologically effective (BE) UV-B radiation following these reductions in O 3 layer range from 30-60 (kJ m K2 d K1) BE up to 50-100 (kJ m K2 d K1) BE . These ranges of daily UV-B doses appear sufficient to exert mutagenic effects on plants, especially if sustained over tens of thousands of years, unlike either rising temperatures or SO 2 concentrations.

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Homologous recombination in plants is temperature and day-length dependent

Cited by 53 publications

References 44 publications

Reevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter Lines

Reevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter Lines

Transgenerational Stress Memory Is Not a General Response in Arabidopsis

The stability of the stratospheric ozone layer during the end-Permian eruption of the Siberian Traps

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