Homologous recombination and RecA protein: towards a new generation of tools for genome manipulations

Volodin, Alexander M.; Voloshin, Oleg N.; Camerini‐Otero, R. Daniel

doi:10.1016/j.tibtech.2004.12.005

Cited by 12 publications

(6 citation statements)

References 76 publications

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“…We made this comment based on two facts. (i) The Cre/loxP system has successfully used for genomic engineering in a wide range of organisms, and (ii) RecA is a ubiquitous protein present in all organisms ranging from bacteria to humans and RecA-mediated homologous recombination is essential for maintaining genomic integrity and for generating genetic diversity 34 . Secondly, the ability of this strategy to clone large DNA fragment will not disappoint natural product researchers.…”

Section: Discussionmentioning

confidence: 99%

“Cre/loxP plus BAC”: a strategy for direct cloning of large DNA fragment and its applications in Photorhabdus luminescens and Agrobacterium tumefaciens

Liu

Zhang

et al. 2016

Sci Rep

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Heterologous expression has been proven to be a valid strategy for elucidating the natural products produced by gene clusters uncovered by genome sequencing projects. Efforts have been made to efficiently clone gene clusters directly from genomic DNA and several approaches have been developed. Here, we present an alternative strategy based on the site-specific recombinase system Cre/loxP for direct cloning gene clusters. A type three secretion system (T3SS) gene cluster (~32 kb) from Photorhabdus luminescens TT01 and DNA fragment (~78 kb) containing the siderophore biosynthetic gene cluster from Agrobacterium tumefaciens C58 have been successfully cloned into pBeloBAC11 with “Cre/loxP plus BAC” strategy. Based on the fact that Cre/loxP system has successfully used for genomic engineering in a wide range of organisms, we believe that this strategy could be widely used for direct cloning of large DNA fragment.

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Section: Discussionmentioning

confidence: 99%

“Cre/loxP plus BAC”: a strategy for direct cloning of large DNA fragment and its applications in Photorhabdus luminescens and Agrobacterium tumefaciens

Liu

Zhang

et al. 2016

Sci Rep

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“…The advantages of our approach are including homologous recombination in one step using a single construct and high efficient transgene transferring into the target cells with a minimal RI rate. A Cre/LoxP approach can be further used in clinical studies to remove post HR any residual selection marker genes (Volodin et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Towards β-globin gene-targeting with integrase-defective lentiviral vectors

et al. 2010

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We have developed an integrase-defective lentiviral (LV) vector in combination with a gene-targeting approach for gene therapy of β-thalassemia. The β-globin gene-targeting construct has two homologous stems including sequence upstream and downstream of the β-globin gene, a β-globin gene positioned between hygromycin and neomycin resistant genes and a herpes simplex virus type 1 thymidine kinase (HSVtk) suicide gene. Utilization of integrase-defective LV as a vector for the β-globin gene increased the number of selected clones relative to non-viral methods. This method represents an important step toward the ultimate goal of a clinical gene therapy for β-thalassemia.

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“…Homologous recombination (HR) is an essential process during which gene rearrangement in meiosis and double-strand break repair of stalled replication forks takes place 86 - 88 . The AAA+ domain of yeast RuvB1 and RuvB2 are weakly homologous to the bacterial RuvB helicase.…”

Section: Functional Insight: Ruvb Family Of Proteins Are Involved In mentioning

confidence: 99%

Plasmodium falciparumRuvB proteins

Ahmad

Tuteja

2012

Communicative & Integrative Biology

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The urgent requirement of next generation antimalarials has been of recent interest due to the emergence of drug-resistant parasite. The genome-wide analysis of Plasmodium falciparum helicases revealed three RuvB proteins. Due to the presence of helicase motif I and II in PfRuvBs, there is a high probability that they contain ATPase and possibly helicase activity. The Plasmodium database has homologs of several key proteins that interact with RuvBs and are most likely involved in the cell cycle progression, chromatin remodeling, and other cellular activities. Phylogenetically PfRuvBs are closely related to Saccharomyces cerevisiae RuvB, which is essential for cell cycle progression and survival of yeast. Thus PfRuvBs can serve as potential drug target if they show an essential role in the survival of parasite.

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Homologous recombination and RecA protein: towards a new generation of tools for genome manipulations

Cited by 12 publications

References 76 publications

“Cre/loxP plus BAC”: a strategy for direct cloning of large DNA fragment and its applications in Photorhabdus luminescens and Agrobacterium tumefaciens

“Cre/loxP plus BAC”: a strategy for direct cloning of large DNA fragment and its applications in Photorhabdus luminescens and Agrobacterium tumefaciens

Towards β-globin gene-targeting with integrase-defective lentiviral vectors

Plasmodium falciparumRuvB proteins

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