Homologous overexpression of xylanase in Fusarium oxysporum increases ethanol productivity during consolidated bioprocessing (CBP) of lignocellulosics

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

“…Two transformants showed marginally higher extracellular xylanase activities (5-10%) compared to the wild type, a significant increase in xylanase transcript was observed though. These strains produced about 60% more ethanol compared to the wild type on corn cob, while for wheat bran this increase was observed only for one of the strains (Anasontzis et al, 2011).…”

“…Overexpression of the sugar transporter (Hxt) in F. oxysporum significantly enhanced the glucose and xylose transport capacity and ethanol yield (39% increase), when straw, glucose and xylose were used as carbon source. In another attempt to enhance ethanol productivity, the endo-β-1,4-xylanase 2 gene was incorporated into the F. oxysporum genome under control of the gpdA promoter using Agrobacterium-mediated transformation (Anasontzis et al, 2011). Two transformants showed marginally higher extracellular xylanase activities (5-10%) compared to the wild type, a significant increase in xylanase transcript was observed though.…”

Challenges in ethanol production withFusarium oxysporumthrough consolidated bioprocessing

“…15 For untreated wheat bran, an ethanol yield of 34% of the theoretical was achieved, which was increased to 56% with the constitutive expression of a xylanase in F. oxysporum F3. 9 In another study with brewer's spent grain, F. oxysporum never produced more than 9 g L ¡1 ethanol. In terms of yield based on the total glucose and xylose content of the substrate, it gave 109 g ethanol per kg of dry substrate or 60% of the theoretical.…”

“…7,8 Improvement in its efficiency of ethanol production has even been shown, upon www.landesbioscience.comoverexpression of a native xylanase. 9 F. oxysporum F3 was also the parental strain used in our study.…”

Microorganisms for Biorefining of Green Biomass

“…Zymomonas mobilis (Mohagheghi et al 2002;Deanda et al 1996) Fusarium oxysporum (Anasontzis et al 2011) Escherichia coli (Saha et al 2011) Saccharomyces cerevisiae (Bettiga et al 2009;Verho et al 2003;Johansson and HahnHägerdal 2002) Pichia stipitis (Xavier et al 2010) Klebsiella oxytoca (Golias et al 2002;Karimi et al 2006;Ohta et al 1991) Clostridium acetobutylicum (Li et al 2013 (Rodrussamee et al 2011;Kumar et al 2009) Caloramator boliviensis (Crespo et al 2012) Thermoanaerobacter pentosaceus (Iwasaki et al 1990) Caldicellulosiruptor saccharolyticus (Isern et al 2013) …”