Homologous and Heterologous Antibody Responses of Mice Immunized with Purified Feline Herpesvirus Type 1 and Canine Herpesvirus Glycoproteins.

Limcumpao, Joselito A.; Horimoto, Taisuke; Xuan, Xuenan; Tohya, Yukinobu; Azetaka, Masayuki; Takahashi, Eiji; Mikami, Takeshi

doi:10.1292/jvms.53.423

Cited by 12 publications

(12 citation statements)

References 32 publications

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“…In addition, pME-cgD (YP2)-and pME-fgD-transfected COS-7 cells were recognized by an MAb 25C9 against FHV-1 gD (data not shown). This observation consists with that of previous study using CHVinfected MDCK cells [15]. CHV gD must have at least an epitope similar to that of FHV-1 gD.…”

Section: Expression Of Chv Gd In Cos-7 Cellssupporting

confidence: 85%

“…By using polyvalent and monoclonal antibodies, CHV gB and gD were cross-reactive with FHV-1 gB and gD, respectively [15]. By Southern-blot analysis, it was revealed that genomic DNA of CHV is hybridized to that of FHV-1 [27].…”

Section: Discussionmentioning

confidence: 99%

“…(IFA) and immunoblot analysis, and inhibited HA activity of CHV [15]. CHV agglutinates only canine RBC [23] while FHV-1 agglutinates only feline RBC [6,22].…”

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confidence: 99%

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Characterization of Canine Herpesvirus Glycoprotein D (Hemagglutinin).

Maeda

Xuan

Kawaguchi

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. Glycoprotein D (gD) of canine herpesvirus (CHV) YP2 strain was expressed in COS-7 and insect (Spodoptera frugiperda; Sf9) cells. The gDs expressed in COS-7 and Sf9 cells reacted with a panel of monoclonal antibodies (MAbs) against CHV gD (hemagglutinin) and an MAb 25C9 against feline herpesvirus type 1 (FHV-1) gD by indirect immunofluorescence assay, and possessed a molecular weight (MW) of approximately 51-55 and 41-46 kilodalton (kDa), respectively, when examined by immunoblot analysis. After treatment with tunicamycin, the MW of the gD expressed in Sf9 cells became approximately 37 kDa. By hemadsorption (HAD) tests using canine or feline red blood cells (RBC), COS-7 cells expressing CHV gD adsorbed only canine RBC, but not feline RBC, whereas control COS-7 cells expressing FHV-1 gD adsorbed feline RBC, but not canine RBC. By hemagglutination (HA) tests, lysates of Sf9 cells expressing CHV gD agglutinated canine RBC, but not feline RBC. These HA and HAD activities were inhibited by HA-inhibition MAbs against CHV gD. Control lysates of Sf9 cells expressing FHV-1 gD agglutinated only feline RBC. Serum from mice inoculated with lysates of Sf9 cells expressing CHV gD possessed a high titer of virus-neutralizing activities against CHV infection. These results indicated that CHV gD is structurally similar to FHV-1 gD, but is functionally different from FHV-1 gD. -KEY WORDS : canine herpesvirus, feline herpesvirus type 1, glycoprotein D, hemagglutinin.

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Section: Expression Of Chv Gd In Cos-7 Cellssupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

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Characterization of Canine Herpesvirus Glycoprotein D (Hemagglutinin).

Maeda

Xuan

Kawaguchi

et al. 1997

The Journal of Veterinary Medical Science

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“…Similar methods of antigen preparation have been used for FHV-1 ELISA development. 25 The results of the ELISA using HSV nuclear and purified viral antigens correlated closely with SNT results and appeared useful for serologic diagnosis of HSV infection. However, a more convenient serodiagnostic ELISA was needed, using nuclear antigen for the detection of HSV infection because ultracentrifugation is not an efficient means for preparing nuclear antigen.…”

Section: Discussionmentioning

confidence: 53%

An Enzyme-Linked Immunosorbent Assay Using Nuclear Antigen for Detection of Feline Herpesvirus 1 Antibody

et al. 1999

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Abstract. To detect antibody against feline herpesvirus 1 (FHV-1) in the sera of cats, the sensitivity and specificity of an enzyme-linked immunosorbent assay (ELISA) using nuclear antigen was investigated. The standardized optical density readings (ODs) of the ELISA obtained by the 1-step serum dilution (1:80) method were compared with the serum neutralization test (SNT) results, with a correlation of 0.993, and with the hemagglutination inhibition (HI) test results, with a correlation of 0.851. The ODs for the ELISA titers were obtained using the serial serum dilution method and were compared with the SNT results, with a correlation of 0.933, and with the HI test results, with a correlation of 0.987. In the experimental infection of 4 specificpathogen-free cats, the results of different serologic tests (SNT and HI) and the ELISA using the serial serum dilution method revealed rapid production of antibodies after inoculation, whereas the ELISA using the onestep serum dilution method indicated that titers increased more slowly. These results indicate that with the present ELISA using nuclear antigen, there are fewer demands on time and labor, making the method convenient for monitoring FHV-1 infection.Feline herpesvirus 1 (FHV-1) was first isolated in 1958. 8 The virus causes upper respiratory disease or, in some cases, keratitis. 33 After clinical manifestations, cats recover from the disease, become subclinical virus carriers, and have recurrent infections. In some cases, kittens are infected by the queen. 17 In catteries, pet shops, pet hotels, etc., where cats are kept in large numbers, the detection of cats with clinical manifestations or inapparent FHV-1 infection and the postvaccination antibody level are considered important for the control and prevention of the disease.The serum neutralization test (SNT) is the most common serologic test used for the detection of FHV-1-infected cats, 3,7,9,31,32 but the serum neutralizing antibody to FHV-1 is difficult to detect by conventional SNT because of the poor immunogenicity of FHV-1. SNTs have been somewhat improved by the addition of complement. These tests either have detected only high titers 21 or have not been effective. 32 The hemagglutination inhibition (HI) test gives a higher titer than SNT 13 and is useful for detection of antibody. 31 The fluorescent antibody technique, 5 when compared with the HI test, provides similar results (G. Suzuki, personal communication).Enzyme-linked immunosorbent assay (ELISA) has been used for diagnosis of many viral diseases, 12,34 in-

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“…A competitive enzyme-linked immunosorbent assay (ELISA) with MAbs reveals at least four different epitopes on the gD [35]. Affinity-purified gD induces VN antibodies in mice [37], and high titers of VN antibodies are also generated in rabbits inoculated with the recombinant vaccinia virus expressing gD [76]. Additionally, mice immunized with recombinant baculovirus-infected cells expressing gD, possess high titers of VN antibodies [44].…”

Section: Viral Glycoproteinsmentioning

confidence: 99%

Properties and Functions of Feline Herpesvirus Type 1 Glycoproteins.

1998

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ABSTRACT. Feline herpesvirus type 1 (FHV-1) is a causative agent of feline viral rhinotracheitis and belongs to the subfamily Alphaherpesvirinae of the family Herpesviridae. Since first isolated in 1958 by Crandell and Maurer, FHV-1 is distributed worldwide and is the most clinically significant agent for respiratory infections in cats. In this review, we describe the recent findings with properties and functions of FHV-1 glycoproteins, especially hemagglutinins. -KEY WORDS: feline herpesvirus type 1, glycoprotein, hemagglutinin.J. Vet. Med. Sci. 60(8): 881-888, 1998 negative staining of the virions [16,52,54,88] or ultrathin sectioning of FHV-1-infected cells [11,54], mature virus particles are 120-180 nm in diameter, with various morphologies in shapes and sizes of the envelope surrounding the hexagonal outline of the capsid. The genome of FHV-1 consists of approximately 126-134 kbp. It has a G+C content of approximately 50%. The genome is divided into two unique portions, the unique long (U L ) and unique short (U S ) regions, by inverted repeat segments (IR) (Fig. 1) [24,70]. The U L is 99-104 kbp in size and is composed of unique DNA. The adjacent short segment is approximately 27-30 kbp in size and contains a central portion of Us which is approximately 8-9 kbp in size. The Us region is bounded by inverted repeat sequences of 7-11 kbp.We have already introduced review articles on recombinant viral vector vaccines for the veterinary use [97] and molecular interactions between retroviruses and herpesviruses [30]. Therefore, this review mainly focuses on some of the recent developments regarding properties and functions of FHV-1 glycoproteins including hemagglutinin.

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Homologous and Heterologous Antibody Responses of Mice Immunized with Purified Feline Herpesvirus Type 1 and Canine Herpesvirus Glycoproteins.

Cited by 12 publications

References 32 publications

Characterization of Canine Herpesvirus Glycoprotein D (Hemagglutinin).

Characterization of Canine Herpesvirus Glycoprotein D (Hemagglutinin).

An Enzyme-Linked Immunosorbent Assay Using Nuclear Antigen for Detection of Feline Herpesvirus 1 Antibody

Properties and Functions of Feline Herpesvirus Type 1 Glycoproteins.

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