Abstract:DNA‐DNA hydridization has been used to detect homologies between the TOL plasmid‐encoded gene cluster xylXYZL and the chromosomally‐located benABCD genes in Pseudomonas putida; both sets of genes code for isofunctional enzymes converting benzoate and toluates into catechol and its derivatives. A DNA probe corresponding to a region downstream of xylL, however, failed to hybridize to Pseudomonas chromosomal DNA. These results support the notion that catabolic operons may evolve by successive recruitment of other… Show more
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