Homogeneous, Rapid Luminescent Oxygen Channeling Immunoassay (LOCITM) for Homocysteine

Liu, Yen Ping; Keczer, Steve de; Alexander, Svetlana; Pirio, Marcel R.; Davalian, Dariush; Kurn, Nurith; Ullman, Edwin F.

doi:10.1093/clinchem/46.9.1506

Cited by 8 publications

(3 citation statements)

References 5 publications

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“…Thus, this HCMV IgG LICA could accurate determine HCMV IgG serum levels with the presence of HCMV IgM. Moreover, this LICA analysis system of HCMV IgG is a homogeneous immunoassay, providing a liquid three‐dimensional reaction environment, in which steric hindrance effect does not exist 33,34 . This system exhibited good precision and sensitivity.…”

Section: Discussionmentioning

confidence: 94%

Establishment of a sandwich light‐initiated chemiluminescence assay with double antigen for detecting human cytomegalovirus IgG antibody

Liu

Líu

et al. 2021

Journal of Medical Virology

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Determination of human cytomegalovirus IgG (HCMV IgG) level is of great importance in the diagnosis of HCMV infections. In this study, a novel, double antigen sandwich homogeneous immunoassay‐based light‐initiated chemiluminescent assay (LICA) for measuring HCMV IgG serum levels was developed. This sandwich LICA for HCMV IgG was performed by incubating serum samples with HCMV pp150 protein coated with chemibeads, streptavidin‐coated sensibeads, and biotinylated HCMV pp150 protein. The working conditions of this assay were optimized and the correlation between the results of the LICA and enzyme‐linked immunosorbent assay was evaluated. As a homogeneous immunoassay, this sandwich LICA could accurately and rapidly determine the serum levels of HCMV IgG with a high‐throughput. Thus, this newly developed assay could be a useful analytical tool in the clinical diagnosis of HCMV infections.

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Section: Discussionmentioning

confidence: 94%

Establishment of a sandwich light‐initiated chemiluminescence assay with double antigen for detecting human cytomegalovirus IgG antibody

Liu

Líu

et al. 2021

Journal of Medical Virology

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“…AlphaScreen technology was first described in 1994 and is based on the principle of luminescent oxygen channeling [ 186 , 187 ]. AlphaScreen is a bead-based, nonradioactive amplified luminescent proximity homogeneous assay in which a donor and an acceptor pair of 250-nm-diameter reagent-coated polystyrene microbeads are brought into proximity by a molecular interaction of binding partners immobilized to these beads [ 187 , 188 , 189 ]. The detection system of AlphaScreen can be time-gated, as the signal is long lived, thus, eliminating short-lived background signals.…”

Section: Strategies For the Discovery Of Novel Ligands For Nuclearmentioning

confidence: 99%

Targeting Nuclear Receptors with Marine Natural Products

Yang

2014

Marine Drugs

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Nuclear receptors (NRs) are important pharmaceutical targets because they are key regulators of many metabolic and inflammatory diseases, including diabetes, dyslipidemia, cirrhosis, and fibrosis. As ligands play a pivotal role in modulating nuclear receptor activity, the discovery of novel ligands for nuclear receptors represents an interesting and promising therapeutic approach. The search for novel NR agonists and antagonists with enhanced selectivities prompted the exploration of the extraordinary chemical diversity associated with natural products. Recent studies involving nuclear receptors have disclosed a number of natural products as nuclear receptor ligands, serving to re-emphasize the translational possibilities of natural products in drug discovery. In this review, the natural ligands of nuclear receptors will be described with an emphasis on their mechanisms of action and their therapeutic potentials, as well as on strategies to determine potential marine natural products as nuclear receptor modulators.

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“…Nanomaterials and nanoprobes play irreplaceable roles in the diagnosis of a range of diseases. , Owing to the demand of higher sensitivity, fluorescence, chemiluminescence, and electrochemiluminescence, immunoassays are gradually replacing conventional colloidal gold method and radioimmunoassay, − which have been widely used in the diagnosis and prognosis of diseases such as tumors, diabetes, and acute myocardial infarction. − For example, clinical biochemical analyzers usually employ streptavidin–biotin system to cross-link magnetic particles and antibodies, converting the concentration of antigen into chemiluminescence or electrochemiluminescence signals. Here, nanomaterials act as both protein carriers and signal amplifiers .…”

Section: Introductionmentioning

confidence: 99%

Antibody-Oriented Strategy and Mechanism for the Preparation of Fluorescent Nanoprobes for Fast and Sensitive Immunodetection

Lou

Fan

et al. 2019

Langmuir

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Nanoprobes have been widely used in biomedical engineering. However, antibodies are generally conjugated onto nanoparticles disorderly, which reduces their antigen recognition ability. The existing antibody orientation approaches are usually complex. Here, we developed and demonstrated a simple antibody-oriented strategy for the lateral flow immunoassay of cardiac troponin I by conjugating antibodies onto polystyrene nanospheres at the optimal pH. The binding amount and orientation of antibodies as well as the detection sensitivity were significantly improved. Although pH regulation is commonly used to optimize antibody conjugation, this paper illustrates the mechanism of its antibody orientation enhancement ability for the first time and reveals the important influences of the density, the charge distribution and hydrophilicity of the antibody, the control of the velocities of physical adsorption and chemical coupling, and other factors on antibody orientation. It is of great significance to understand and regulate antibody conjugation on the surface of micro-or nanospheres to construct high-performance probes for in vitro diagnosis applications.

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Homogeneous, Rapid Luminescent Oxygen Channeling Immunoassay (LOCITM) for Homocysteine

Cited by 8 publications

References 5 publications

Establishment of a sandwich light‐initiated chemiluminescence assay with double antigen for detecting human cytomegalovirus IgG antibody

Establishment of a sandwich light‐initiated chemiluminescence assay with double antigen for detecting human cytomegalovirus IgG antibody

Targeting Nuclear Receptors with Marine Natural Products

Antibody-Oriented Strategy and Mechanism for the Preparation of Fluorescent Nanoprobes for Fast and Sensitive Immunodetection

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