Homogeneous immunoassay for human IgG using oriented hen egg IgY immobilized on gold sol nanoparticles

Yeritsyan, Hermine E.; Gasparyan, Vardan K.

doi:10.1007/s00604-011-0703-3

Cited by 14 publications

(8 citation statements)

References 32 publications

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“…Because an ordered display of proteins is often more favored, both covalent and non-covalent strategies to orient proteins uniformly on surfaces have been developed. Covalent approaches have taken advantage of maleimide reactivity with thiols, native chemical ligation, photochemical thiol–ene chemistry, carbohydrate moieties, Si-tags, and enzymatic tags, , to name a few. Representative non-covalent systems are exemplified by polyhistidine tag incorporation via genetic engineering to bind to Ni-NTA-functionalized surfaces, as well as biotin–streptavidin complexation. − Another non-covalent protein immobilization approach is through the use of DNA, taking advantage of complementary strand hybridization.…”

Section: Introductionmentioning

confidence: 99%

Site-Selective Oxidative Coupling Reactions for the Attachment of Enzymes to Glass Surfaces through DNA-Directed Immobilization

et al. 2017

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Enzymes are able to maintain remarkably high selectivity toward their substrates while still retaining high catalytic rates. By immobilizing enzymes onto surfaces we can heterogenize these biological catalysts, making it practical to study, use, and combine them in an easily controlled system. In this work, we developed a platform that allows for the simple and oriented immobilization of proteins through DNA-directed immobilization. First, we modified a glass surface with single-stranded DNA. We then site-selectively attached the complementary DNA strand to the N-terminus of a protein. Both DNA modifications were carried out using an oxidative coupling strategy, and the DNA strands served as easily tunable and reversible chemical handles to hybridize the protein-DNA conjugates onto the surface. We have used the aldolase enzyme as a model protein to conduct our studies. We characterized each step of the protein immobilization process using fluorescent reporters as well as atomic force microscopy. We also conducted activity assays on the surfaces with DNA-linked aldolase to validate that, despite being modified with DNA and undergoing subsequent immobilization, the enzyme was still able to retain its catalytic activity and the surfaces were reusable in subsequent cycles.

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Section: Introductionmentioning

confidence: 99%

Site-Selective Oxidative Coupling Reactions for the Attachment of Enzymes to Glass Surfaces through DNA-Directed Immobilization

et al. 2017

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“…The increase in uorescence depended on the concentration of hIgG, resulting in the sensitive detection of hIgG concentration. Compared with previous reports, [4][5][6] the current work is advantageous over others in two signicant aspects: rstly, this novel platform has a detection range of 4.0 Â 10 À9 to 2.2 Â 10 À7 g mL À1 , which is an obvious improvement in detection range and sensitivity, and secondly, this platform only requires goat anti-IgG antibody labeled FITC, reducing the labor required for the quencher labeling. Photoluminescence (PL) spectra were obtained on a Shimadzu RF-5301 PC spectra uorophotometer.…”

Section: Introductionmentioning

confidence: 89%

“…H. E. Yeritsyan et.al. 4 applied oriented immobilization of hen egg IgY on gold nanoparticles when developing a UV-Vis absorption spectra method for the analysis of hIgG with a detection range of 30-500 ng mL À1 . Although this method has a wide detection range and a low detection limit, this procedure needs a great deal of time to obtain relatively pure IgY.…”

Section: Introductionmentioning

confidence: 99%

Detection of immunoglobulin G based on nanoparticle surface energy transfers from fluorescein isothiocyanate to gold nanoparticles

Tao

Liao

et al. 2014

Anal. Methods

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In this paper, a simple and sensitive approach for human immunoglobulin G (hIgG) detection is described based on nanoparticle surface energy transfer (NSET) from fluorescein isothiocyanate (FITC) to gold nanoparticles (GNPs). The assay consisted of polyclonal goat anti-IgG antibody labeled luminescent FITC as the donor and GNPs as the acceptor. In the initial stage, with the energy transfer from FITC to GNPs, the FITC fluorescence was effectively quenched. Upon the introduction of IgG, effective competitive binding to the polyclonal goat anti-IgG antibody labeled FITC occurred, which significantly hindered the NSET, and thus recovered the fluorescence of FITC. The change in fluorescent intensity produced a novel strategy for the detection of hIgG. The recovered fluorescence of FITC was linearly proportional to the concentration of hIgG in the range of 4.0 Â 10 À9 to 2.2 Â 10 À7 g mL À1 with a detection limit of 8.3 Â 10 À10 g mL À1 . This method was applied to the determination of hIgG in human serum samples via a standard addition method, and the recovery rate obtained was 98.7-105.0%.

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“…Also, in immunehistochemistry, Xiao & Gao [7] have shown that combining the high sensitivity and specificity of IgY and the high brightness and photo stability of quantum dots can improve detection and quantitation of biomarkers. In another study, homogeneous immunoassay for human IgG using oriented hen egg IgY immobilized on gold sol nanoparticles has been presented with a wide detection range and a limit of detection of 30ng.mL-1 of human IgG [8].…”

Section: Nanomedicine For Therapeutics and Drug Delivery Of Igymentioning

confidence: 99%

Egg Yolk Antibodies and Nanotechnology Applied to Animal Health and Production

Bellingeri

Picco

Alustiza

et al. 2018

APDV

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Homogeneous immunoassay for human IgG using oriented hen egg IgY immobilized on gold sol nanoparticles

Cited by 14 publications

References 32 publications

Site-Selective Oxidative Coupling Reactions for the Attachment of Enzymes to Glass Surfaces through DNA-Directed Immobilization

Site-Selective Oxidative Coupling Reactions for the Attachment of Enzymes to Glass Surfaces through DNA-Directed Immobilization

Detection of immunoglobulin G based on nanoparticle surface energy transfers from fluorescein isothiocyanate to gold nanoparticles

Egg Yolk Antibodies and Nanotechnology Applied to Animal Health and Production

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