Homogeneous hybrid droplet interface bilayers assembled from binary mixtures of DPhPC phospholipids and PB-b-PEO diblock copolymers

Koner, Subhadeep; Tawfik, J; Mashali, Farzin; Kennison, Kristen B.; McClintic, William T.; Heberle, Frederick A.; Tu, Yu-Ming; Kumar, Manish; Sarles, Stephen A.

doi:10.1016/j.bbamem.2022.183997

Cited by 2 publications

(2 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…52 Thus, this combined capability makes for a more comprehensive experimental approach to study the physicochemical features of lipid bilayers and gain mechanistic insights into NP−bilayer interactions. 48,53 Nonetheless, the DIB approach also presents a challenge. While AmNPs are soluble in water, 54 we learned that adding them to the aqueous droplet solution prior to lipid monolayer assembly or bilayer formation in oil consistently leads to membrane rupture and droplet coalescence.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Imaging the connected droplet pair also allows for assessing the contact angle between droplets, a parameter that depends on lateral bilayer tension . Thus, this combined capability makes for a more comprehensive experimental approach to study the physicochemical features of lipid bilayers and gain mechanistic insights into NP–bilayer interactions. , …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous Electrophysiology and Imaging Reveal Changes in Lipid Membrane Thickness and Tension upon Uptake of Amphiphilic Gold Nanoparticles

Mashali,

Basham,

et al. 2023

Langmuir

Self Cite

View full text Add to dashboard Cite

Amphiphilic gold core nanoparticles (AmNPs) striped with hydrophilic 11-mercapto-1-undecanesulfonate (MUS) and hydrophobic 1-octanethiol (OT) ligands are promising candidates for drug carriers that passively and nondisruptively enter cells. Yet, how they interact with cellular membranes is still only partially understood. Herein, we use electrophysiology and imaging to carefully assess changes in droplet interface bilayer lipid membranes (DIBs) incurred by striped AmNPs added via microinjection. We find that AmNPs spontaneously reduce the steady-state specific capacitance and contact angle of phosphatidylcholine DIBs by amounts dependent on the final NP concentration. These reductions, which are greater for NPs with a higher % OT ligands and membranes containing unsaturated lipids but negligible for MUS-only-coated NPs, reveal that AmNPs passively embed in the interior of the bilayer where they increase membrane thickness and lateral tension through disruption of lipid packing. These results demonstrate the enhanced evaluation of nano–bio interactions possible via electrophysiology and imaging of DIBs.

show abstract