Homogeneous assay based on low quantum yield Sm(III)-donor and anti-Stokes’ shift time-resolved fluorescence resonance energy-transfer measurement

Laitala, Ville; Hemmilä, Ilkka

doi:10.1016/j.aca.2005.07.012

Cited by 9 publications

(17 citation statements)

References 16 publications

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“…In a study of DNA hybridization with labeled oligonucleotides, we have found that samarium shows unexpectedly high energy transfer kinetics and good signals applicable in proximity assay at a short distance, such as oligonucleotide hybridization [33]. Regardless of a very low overlapping integral between samarium and Alexa-Fluor 532, the sensitivity of homogeneous delta-F508 assay was very high, allowing 12 pM of the amplified sequence to be detected, demonstrating a very efficient energy transfer emanating directly from the 4 F 3/2 or 4 G 5/2 levels (Fig.…”

Section: Other Lanthanidesmentioning

confidence: 99%

“…4). In a study using different Alexa dyes in hybridization assay with samarium-labeled probe, the energy transfer from the higher excited states produced longer and stable decay time with nonoverlapping ET pair (samarium and Alexa Fluors À488, À514 and À532) as compared to traditional overlapping pair (Sm and Alexa Fluor 647) (6.6-8.5 ms as compared with 1.3 ms with Alexa-647) [33].…”

Section: Other Lanthanidesmentioning

confidence: 99%

“…Delay optimization is particularly important in applications where the TR-FRET signal has a short decay time or the donor quenching is to be measured. Optimized delay time prevents losing the acceptor signal in case of efficient FRET and, correspondingly, more efficient donor quenching can be measured using adequate delay times [33,48]. Short measurement windows can be utilized to further suppress background coming from the long-decay cross-talk of donor signal through acceptor filter.…”

Section: Gated Detection and Signal Processingmentioning

confidence: 99%

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Sensitized Bioassays

Hemmilä¹,

Laitala²

2010

Lanthanide Luminescence

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Luminescent lanthanides are well-suited and widely applied markers for bioaffinity assays, both separation-based heterogeneous assays, such as immunoassays, as well as for homogeneous assays, such as assays based on energy transfer. Their properties allow efficient combination of spectral and temporal resolution to gain good sensitivity, perfect discrimination of produced sensitized signal, and robust assays. Both europium and terbium, as their stable and highly luminescent chelates, have found wide applications in research aiming for new drug discovery. The complicated but efficient energy transfer process in the chelates, and between chelate and acceptor, provides versatile tools to stipulate good assays for various applications and also to gain limited multiplexing to allow measurement of several biomarkers simultaneously. The chapter reviews the recent development in the area and gives overview of interesting applications.

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Section: Other Lanthanidesmentioning

confidence: 99%

Section: Other Lanthanidesmentioning

confidence: 99%

Section: Gated Detection and Signal Processingmentioning

confidence: 99%

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Sensitized Bioassays

Hemmilä¹,

Laitala²

2010

Lanthanide Luminescence

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“…Some europium(III) chelate-dyed nanoparticles can even be excited above 400 nm wavelength [51,147,148], which, together with the development of UV-LED technology, may change the situation in the near future. Further advantage of UV-LED (and also N 2 laser) excitation is the rapid decay of the excitation pulse which, in the case of Xe flash lamps, can limit the detection of the very rapidly decaying emission of dysprosium(III) and samarium(III) [89] or of the energy-transfer excited emission with short apparent decay time in TR-FRET (time-resolved fluorescence resonance energy-transfer) applications [149].…”

Section: Detection Instrumentationmentioning

confidence: 99%

Lanthanide Nanoparticules as Photoluminescent Reporters

Soukka

Härmä

2010

Lanthanide Luminescence

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Luminescent lanthanide nanoparticles provide a combination of high luminescence intensity, long luminescence lifetime, sharp emission peaks with narrow bandwidth, and a large Stokes' shift, leading to a high-performance reporter technology for bioanalytical assays. The high specific activity of luminescent lanthanide nanoparticles enables improved assay sensitivities compared to the conventional fluorescent reporters, and the different luminescent lanthanide ions with well-separated emission wavelengths facilitate multiparametric assays. The versatility of lanthanide chelate-doped polymeric nanoparticles as reporters is further increased by the use of the most effective organic light-harvesting ligands optimized for enhanced luminescence and the availability of inexpensive highpower solid-state light sources. Moreover, entrapping within polymeric nanoparticles allows the use of the lanthanide complexes with a weak water solubility or a low thermodynamic stability. In this review, we discuss the effects of nanoparticle composition, particle size, synthesis, surface modification, and biomolecule conjugation on the assay performance in addition to applications of these reporters in both heterogeneous and homogeneous assays. In the future, additional value may be provided by hybrid lanthanide nanoparticles, which combine, e.g., luminescent and magnetic properties for detection and controlling the assay kinetics and washing efficiency.

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“…According to the traditional definition of FRET, yet another requirement is a substantial spectral overlap between the donor emission and acceptor excitation spectra. However, according to recent studies, this is not an absolute requirement for non-radiative energy transfer, since a phenomenon termed non-overlapping FRET (nFRET; also called anti-Stokes shift FRET) has been described (Laitala and Hemmilä, 2005a;Laitala and Hemmilä, 2005b). The nFRET phenomenon is based on non-radiative energy transfer between a lanthanide donor and a spectrally non-overlapping acceptor.…”

Section: Resonance Energy Transfer To An Acceptor Fluorophorementioning

confidence: 99%

Luminescent lanthanide reporters: new concepts for use in bioanalytical applications

Vuojola

Soukka

2014

Methods Appl. Fluoresc.

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Lanthanides represent the chemical elements from lanthanum to lutetium. They intrinsically exhibit some very exciting photophysical properties, which can be further enhanced by incorporating the lanthanide ion into organic or inorganic sensitizing structures. A very popular approach is to conjugate the lanthanide ion to an organic chromophore structure forming lanthanide chelates. Another approach, which has quickly gained interest, is to incorporate the lanthanide ions into nanoparticle structures, thus attaining improved specific activity and a large surface area for biomolecule immobilization. Lanthanide-based reporters, when properly shielded from the quenching effects of water, usually express strong luminescence emission, multiple narrow emission lines covering a wide wavelength range, and exceptionally long excited state lifetimes enabling time-gated luminescence detection. Because of these properties, lanthanide-based reporters have found widespread applications in various fields of life. This review focuses on the field of bioanalytical applications. Luminescent lanthanide reporters and assay formats utilizing these reporters pave the way for increasingly sensitive, simple, and easily automated bioanalytical applications.

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Homogeneous assay based on low quantum yield Sm(III)-donor and anti-Stokes’ shift time-resolved fluorescence resonance energy-transfer measurement

Cited by 9 publications

References 16 publications

Sensitized Bioassays

Sensitized Bioassays

Lanthanide Nanoparticules as Photoluminescent Reporters

Luminescent lanthanide reporters: new concepts for use in bioanalytical applications

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