Homogeneous and Universal Detection of Various Targets with a Dual‐Step Transduced Toehold Switch Sensor

Abstract

Figure 3. Detectionoft hrombin. A) Schematic representationo fS Dt ransduction (Step I) for thrombin detection.B inding of the thrombin to the Blocker to release TP1. B) 12 %native PAGE analysis of hybridization between Blocker and TP1 with and withoutt hrombin. Lane Mt olane 3: ladder,TP1, Blocker and TP1,Blocker,TP1, and thrombin. C) Specificityc haracterization with ab ar graph representing endpoint detection at 2hcolor reaction.

End-to-end computational approach to the design of RNA biosensors for detecting miRNA biomarkers of cervical cancer

“…The plot of intensity against the complex concentration over a 2-h interval obtained a sigmoidal fit with a steep rise between 10 nM and 100 nM (Section S4, Supplementary File S7). This showed that the model is sensitive to changes in the concentration of the trigger miRNA biomarker in the range of interest, satisfying a necessary biosensor property [ 25 , [55] , [56] , [57] , [58] , [59] , [60] ]. A similar trend of sigmoidal kinetics was observed for the hsa-miR-20a-5p biomarker over a complex concentration range of 100 nM and 1 μM (Supplementary File S7).…”