Homogeneous and Sensitive Detection of microRNA with Ligase Chain Reaction and Lambda Exonuclease-Assisted Cationic Conjugated Polymer Biosensing

Zheng, Yawen; Zhou, Yuanyuan; Gao, Shuxin; Cheng, Yongqiang; Li, Zhengping

doi:10.1021/am500883q

Cited by 67 publications

(36 citation statements)

References 29 publications

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“…Increasing evidence has indicated that abnormal expressions of specific miRNAs show close correlation to many important diseases, including human cancers and other diseases, and these specific miRNAs can be considered to be effective biomarkers for the diagnosis of different diseases. Because of the clinical significance of miRNAs, a series of bulk measurements have been developed to detect specific miRNA levels following isolation from cells, including traditional northern blotting, reverse transcription quantitative PCR (RT‐qPCR), and microarrays with modern fluorescent and electrochemical techniques . However, detection of the average miRNA expression in cell populations could lead to the loss of important information connecting miRNA expression with cell function.…”

Section: Methodsmentioning

confidence: 99%

Live‐Cell MicroRNA Imaging through MnO₂ Nanosheet‐Mediated DD‐A Hybridization Chain Reaction

Huang

Yang

et al. 2017

ChemBioChem

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Innovative techniques to visualize native microRNAs (miRNAs) in live cells can dramatically impact current research on the roles of miRNA in biology and medicine. Here, we report a novel approach for live-cell miRNA imaging using a biodegradable MnO nanosheet-mediated DD-A FRET hybridization chain reaction (HCR). The MnO nanosheets can adsorb DNA hairpin probes and deliver them into live cells. After entering cells, the MnO nanosheets are degraded by cellular GSH. Then, the target miR-21 triggers cascaded assembly of the liberated hairpin probes into long dsDNA polymers, which brings each two FAMs (donor) and one TAMRA (acceptor) into close proximity to generate significantly enhanced DD-A FRET signals, which was discovered and proven by our previous report. We think the developed approach can serve as an excellent intracellular miRNAs detection tool, which promises the potential for biological and disease studies.

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Section: Methodsmentioning

confidence: 99%

Live‐Cell MicroRNA Imaging through MnO₂ Nanosheet‐Mediated DD‐A Hybridization Chain Reaction

Huang

Yang

et al. 2017

ChemBioChem

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“…Primers termini also contained donor and acceptor dyes to enable FRET once brought into close proximity through the formation of a molecular beacon [108]. Yuan et al developed an integrated LCR-lambda exonuclease-assisted cationic conjugated polymer (CCP) biosensor for miRNA genotyping [109]. The ability of CCPs to harvest and amplify signal was used as a donor in the FRET transfer to fluorophore.…”

Section: Fluorescence Resonance Energy Transfer (Fret)mentioning

confidence: 99%

Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications

Gibriel

Adel

2017

Mutation Research/Reviews in Mutation Research

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Genetic variants have been reported to cause several genetic diseases. Various genotyping assays have been developed for diagnostic and screening purposes but with certain limitations in sensitivity, specificity, cost effectiveness and/or time savings. Since the discovery of ligase chain reaction (LCR) in the late nineties, it became one of the most favored platforms for detecting these variants and also for genotyping low abundant contaminants. Recent and powerful modifications with the integration of various detection strategies such as electrochemical and magnetic biosensors, nanoparticles (NPs), quantum dots, quartz crystal and leaky surface acoustic surface biosensors, DNAzyme, rolling circle amplification (RCA), strand displacement amplification (SDA), surface enhanced raman scattering (SERS), chemiluminescence and fluorescence resonance energy transfer have been introduced to both LCR and ligation based amplifications to enable high-throughput and inexpensive multiplex genotyping with improved robustness, simplicity, sensitivity and specificity. In this article, classical and up to date modifications in LCR and ligation based amplifications are critically evaluated and compared with emphasis on points of strength and weakness, sensitivity, cost, running time, equipment needed, applications and multiplexing potential. Versatile genotyping applications such as genetic diseases detection, bacterial and viral pathogens detection are also detailed. Ligation based gold NPs biosensor, ligation based RCA and ligation mediated SDA assays enhanced detection limit tremendously with a discrimination power approaching 1.5aM, 2aM and 0.1fM respectively. MLPA (multiplexed ligation dependent probe amplification) and SNPlex assays have been commercialized for multiplex detection of at least 48 SNPs at a time. MOL-PCR (multiplex oligonucleotide ligation) has high-throughput capability with multiplex detection of 50 SNPs/well in a 96 well plate. Ligase detection reaction (LDR) is one of the most widely used LCR versions that have been successfully integrated with several detection strategies with improved sensitivity down to 0.4fM.

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“…LCR is employed for two pairs of oligonucleotide probes which are adjacent and perfectly complementary to target miRNA or the ligated DNA strand for performing ligation-based exponential amplification [44]. Upon hybridization to a target miRNA strand, the adjacent probes are ligated by a specific ligase [45] (e.g., T4 RNA ligase 2 [46]) to form a long DNA strand.…”

Section: Lcrmentioning

confidence: 99%

“…With a LCR utilized for exponential amplification of miRNA and lambda exonuclease-assisted cationic conjugated polymer (CCP) for fluorescent signal detection, a homogeneous and sensitive detection of miRNA has been proposed ( Fig. 5.10) [44]. The method is sensitive enough to detect 0.1 fM target miRNA and specific to discriminate a one-base difference of miRNAs.…”

Section: Lcrmentioning

confidence: 99%

miRNA Optical Detection

Zhang

Dong

Tian

2015

SpringerBriefs in Molecular Science

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miRNA is a kind of attractive candidates, as biomarkers, for early cancer diagnosis. Therefore, simple and novel strategies for miRNA detection with high sensitivity and selectivity have great significance not only for the studies of its biological functions but also for clinical cancer diagnosis. Various reliable optical miRNA detection methods have become a class of attractive and paramount for miRNA expression analysis, due to its high sensitivity, wide dynamic range, and multiplexing capabilities. The progress of nanotechnology and nanoscience allows nanomaterial-based signal amplification to develop highly sensitive and selective biosensors for in situ or online detection of miRNA. Molecular biology-based strategies for miRNA detection by amplifying the target or probe with high detection sensitivity are applied in identifying and detecting nucleic acids analysis.Keywords Optical detection · Signal amplification · Noble metal nanoparticles · Transition metal dichalcogenides · Quantum dots · Canbon nanomaterials · Target or probe amplification Nanomaterials-Based miRNA Optical DetectionThe development of nanotechnology and nanoscience renders nanomaterial-based signal amplification great promise in developing highly sensitive and selective biosensors for in situ or online detection of miRNA. Nanomaterials-based biosensors exhibit the significant advantages with high sensitivity, wide dynamic range, and multiplexing capabilities compared to bulk materials-biosensors. Nanoparticles (NPs)-based biosensors show the significant advantages as follows: (i) The NPs allow design of low cost and minimized equipment in point-of-care diagnostics due to the small sizes breaking through the limitation of structure miniaturization. (ii) The direct contact between NPs and the environment lead to accelerated

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Homogeneous and Sensitive Detection of microRNA with Ligase Chain Reaction and Lambda Exonuclease-Assisted Cationic Conjugated Polymer Biosensing

Cited by 67 publications

References 29 publications

Live‐Cell MicroRNA Imaging through MnO₂ Nanosheet‐Mediated DD‐A Hybridization Chain Reaction

Live‐Cell MicroRNA Imaging through MnO₂ Nanosheet‐Mediated DD‐A Hybridization Chain Reaction

Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications

miRNA Optical Detection

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Homogeneous and Sensitive Detection of microRNA with Ligase Chain Reaction and Lambda Exonuclease-Assisted Cationic Conjugated Polymer Biosensing

Cited by 67 publications

References 29 publications

Live‐Cell MicroRNA Imaging through MnO2 Nanosheet‐Mediated DD‐A Hybridization Chain Reaction

Live‐Cell MicroRNA Imaging through MnO2 Nanosheet‐Mediated DD‐A Hybridization Chain Reaction

Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications

miRNA Optical Detection

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Live‐Cell MicroRNA Imaging through MnO₂ Nanosheet‐Mediated DD‐A Hybridization Chain Reaction

Live‐Cell MicroRNA Imaging through MnO₂ Nanosheet‐Mediated DD‐A Hybridization Chain Reaction