Homogeneity of Borrelia japonica and heterogeneity of Borrelia afzelii and ‘Borrelia tanukii' isolated in Japan, determined from ospC gene sequences

Nine Borrelia afzelii strains, which had been isolated from two vectors, Ixodes nipponensis and Apodemus agrarius in Chungju, Korea, were characterized by PCR‐RFLP analyses of ospC genes and rrf (5S)‐rrl (23S) intergenic spacer. DraI restriction patterns of Chungju strains were identical to those of B. afzelii VS461. But MseI restriction patterns of rrf(5S)‐rrl (23S) intergenic spacer genes of KK2, KM4, KK5 differed from those of previously reported B. burgdorferi sensu lato strains. Nine Chungju strains were classified with four distinct ospC RFLP patterns, which differed from the eight ospC RFLP patterns (A‐1 to A‐8) of previously reported B. afzelii. Moreover, five additional restriction patterns were deduced from published ospC sequences of reference strains. These results suggest that Chungju strains are very heterogeneous.

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Characterization of Borrelia afzelii Isolated from Ixodes nipponensis and Apodemus agrarius in Chungju, Korea, by PCR‐RFLP Analyses of ospC Gene and rrf (5S)‐rrl(23S) Intergenic Spacer

Lee

Jung

Lee

et al. 2002

Microbiology and Immunology

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Molecular Typing ofBorrelia burgdorferiSensu Lato: Taxonomic, Epidemiological, and Clinical Implications

et al. 1999

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SUMMARY Borrelia burgdorferi sensu lato, the spirochete that causes human Lyme borreliosis (LB), is a genetically and phenotypically divergent species. In the past several years, various molecular approaches have been developed and used to determine the phenotypic and genetic heterogeneity within the LB-related spirochetes and their potential association with distinct clinical syndromes. These methods include serotyping, multilocus enzyme electrophoresis, DNA-DNA reassociation analysis, rRNA gene restriction analysis (ribotyping), pulsed-field gel electrophoresis, plasmid fingerprinting, randomly amplified polymorphic DNA fingerprinting analysis, species-specific PCR and PCR-based restriction fragment length polymorphism (RFLP) analysis, and sequence analysis of 16S rRNA and other conserved genes. On the basis of DNA-DNA reassociation analysis, 10 different Borrelia species have been described within the B. burgdorferi sensu lato complex: B. burgdorferi sensu stricto, Borrelia garinii, Borrelia afzelii, Borrelia japonica, Borrelia andersonii, Borrelia valaisiana, Borrelia lusitaniae, Borrelia tanukii, Borrelia turdi, and Borrelia bissettii sp. nov. To date, only B. burgdorferi sensu stricto, B. garinii, and B. afzelii are well known to be responsible for causing human disease. Different Borrelia species have been associated with distinct clinical manifestations of LB. In addition, Borrelia species are differentially distributed worldwide and may be maintained through different transmission cycles in nature. In this paper, the molecular methods used for typing of B. burgdorferi sensu lato are reviewed. The current taxonomic status of B. burgdorferi sensu lato and its epidemiological and clinical implications, especiallly correlation between the variable clinical presentations and the infecting Borrelia species, are discussed in detail.

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Homogeneity of Borrelia japonica and heterogeneity of Borrelia afzelii and ‘Borrelia tanukii' isolated in Japan, determined from ospC gene sequences

Cited by 2 publications

References 23 publications

Characterization of Borrelia afzelii Isolated from Ixodes nipponensis and Apodemus agrarius in Chungju, Korea, by PCR‐RFLP Analyses of ospC Gene and rrf (5S)‐rrl(23S) Intergenic Spacer

Characterization of Borrelia afzelii Isolated from Ixodes nipponensis and Apodemus agrarius in Chungju, Korea, by PCR‐RFLP Analyses of ospC Gene and rrf (5S)‐rrl(23S) Intergenic Spacer

Molecular Typing ofBorrelia burgdorferiSensu Lato: Taxonomic, Epidemiological, and Clinical Implications

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