Homogeneity of Antibody Responses in Tuberculosis Patients

Samanich, Karen M.; Belisle, John T.; Laal, Suman

doi:10.1128/iai.69.7.4600-4609.2001

Cited by 70 publications

(101 citation statements)

References 29 publications

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“…Previous studies have reported that M. tuberculosis adapts to its environment by altering the profile of the genes that it expresses. This profile is modulated when the in vivo environment changes [21,22]. Thus selection of antigens of M. tuberculosis based on the antibody response of PTB patients alone might not provide the appropriate materials that can be used for the sero-diagnosis of EPTB.…”

Section: Discussionmentioning

confidence: 99%

Serodiagnosis of tuberculous lymphadenitis using a combination of antigens

Beyene

Franken

Yamuah

et al. 2010

J Infect Dev Ctries

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Background: The diagnosis of extra-pulmonary tuberculosis (EPTB) by conventional methods such as culture and microscopy has low sensitivity and requires an invasive procedure. A simple rapid serological test would be of great value. Methodology: Six antigens (ESAT-6, Ag85A, TB10.4, Rv3881c, lipoarabinomannan (LAM) and Ara 6 -BSA) were tested in an ELISA to detect antigen-specific IgG and IgM antibodies in sera from 54 culture-and histology-confirmed tuberculous lymphadenitis (TBLN) patients as follows: four were HIV seropositive; sera from 25 was smear positive for pulmonary tuberculosis (PTB); 15 were culture-and histologynegative lymphadenitis (non-TBLN) patients; and 22 werehealthy controls (HCs). Results: The sensitivities of the antigens for the detection of IgG in sera of TBLN patients ranged from 4% to 30%. Specificities ranged from 73% to 100% with sera from non-TBLN patients and 91% to 100% with sera from HCs. Sensitivities of the antigens for detection of IgM ranged from 0% to 15% and specificities ranged from 80% to 100% with sera from non-TBLN patients and 91% to 100% with sera from HCs. LAM was the most potent antigen for detection of IgG. When LAM and ESAT-6 were combined, sensitivity increased up to 43% and specificity with non-TBLN was 80% with HC 96%. Conclusions: The study suggests that the combined use of LAM and ESAT-6 for IgG antibody detection in sera from TBLN patients could be a supplement to microscopy of fine-needle aspirate (FNA) to diagnose TBLN among patients suspected of TBLN.

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Section: Discussionmentioning

confidence: 99%

Serodiagnosis of tuberculous lymphadenitis using a combination of antigens

Beyene

Franken

Yamuah

et al. 2010

J Infect Dev Ctries

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“…Some of the values from the literature are shown in table 3 for convenient comparisons between main past investigations. Previous studies have reported lower sensitivities with the recombinant version of the 38-kDa antigen, with sensitivities of the ELISA technique ranging 16-36% for smear-negative patients and 36-67% for smear-positive patients [5,7,19]. CHIANG et al [8] reported that the 38-kDa antigen had a sensitivity in diagnosis of 64%.…”

Section: Discussionmentioning

confidence: 99%

“…In receiver operating curve analysis, when the specificity was set to 100%, the 38-kDa antigen had the highest sensitivity of the antigens studied. SAMANICH et al [19] detected antibodies to the 38-kDa antigen in 57.8% of pulmonary cavitary TB patients. In a recent study in Turkey, ERER et al [18] reported 46.5% sensitivity with 100% specificity in pulmonary TB.…”

Section: Discussionmentioning

confidence: 99%

Humoral immune response against 38-kDa and 16-kDa mycobacterial antigens in tuberculosis

Şenol

Erer²,

Yalcin³

et al. 2006

European Respiratory Journal

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Several ELISA tests based on mycobacterial antigens have been used for the rapid diagnosis of tuberculosis (TB), although demonstration of Mycobacterium tuberculosis in a smear or culture is the most reliable method. In the present study, the diagnostic value of 16-kDa and 38-kDa mycobacterial antigens was investigated in patients who were diagnosed with tuberculosis by clinical and/or bacteriological findings in Turkey.The PATHOZYME-TB Complex Plus commercial ELISA kit was used for measuring immunoglobulin G against 38-kDa and 16-kDa recombinant antigens. Humoral immune response was analysed in a group of 179 TB patients (143 smear-positive, 19 smear-negative, eight lymphadenitis and nine pleuritis), 15 inactive TB cases and in control groups consisting of 40 healthy volunteers and 20 subjects with pulmonary diseases other than TB.The sensitivity, specifity, positive predictive value and negative predictive value of the test were determined at 52.5%, 93.3%, 95.9% and 39.7%, respectively in TB cases. Antibodies were detected at above cut-off level in three (20%) out of 15 subjects with inactive TB.In conclusion, the ELISA test has a very good specifity and an acceptable sensitivity and positive predictive value. It is thought that it could be used in combination with other methods to increase diagnostic accuracy, especially for culture-negative tuberculosis cases, which are difficult to diagnose.

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“…When cavity lesions appear, M. tuberculosis expresses certain antigens that are not expressed in earlier stages of the infection. Depending on the time of diagnosis, assays based on these antigens present different sensitivities, as has been mainly reported for HIV-positive patients who normally do not show cavity lesions [29].…”

Section: Discussionmentioning

confidence: 99%

Combined use of Western blot/ELISA to improve the serological diagnosis of human tuberculosis

Beck¹,

Leite²,

Arruda³

et al. 2005

Braz J Infect Dis

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Two recombinant antigens and a crude bacterial antigen of a wild M. tuberculosis strain were used to detect specific IgG antibodies in sera from 52 patients with pulmonary tuberculosis, confirmed by an acid-fast smear and serum culture of these patients and that of 25 contacts. The patients were not infected with HIV. We evaluated the sensitivity and specificity of ELISA, based on the recombinant TbF6® ® ® ® ® and TbF6/DPEP antigen and a search for reactivity patterns in the Western blot technique, using whole mycobacterium antigen. Serum samples from 22 healthy individuals and from 30 patients with lung diseases other than tuberculosis were used as controls. The best ELISA results were obtained with the TbF6/DPEP antigen combination, which gave 85% sensitivity and 91% specificity. ELISA sensitivity improved from 85% to 92% when the Western blot results were used. Western blot specificity was 100% when antibody reactivity with different antigenic bands was analyzed and associated. The association of TbF6/DPEP antigens used in ELISA with specific patterns of reactivity determined by Western blot can help make an identification when classic methods for the diagnosis of pulmonary tuberculosis are not sufficient.

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Homogeneity of Antibody Responses in Tuberculosis Patients

Cited by 70 publications

References 29 publications

Serodiagnosis of tuberculous lymphadenitis using a combination of antigens

Serodiagnosis of tuberculous lymphadenitis using a combination of antigens

Humoral immune response against 38-kDa and 16-kDa mycobacterial antigens in tuberculosis

Combined use of Western blot/ELISA to improve the serological diagnosis of human tuberculosis

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